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Research on the protection effect of pioglitazone for non-alcoholic fatty liver disease (NAFLD) in rats.
J Zhejiang Univ Sci B. 2006 Aug; 7(8):627-33.JZ

Abstract

OBJECTIVE

The prevalence of non-alcoholic fatty liver disease (NAFLD) has markedly increased. Insulin resistance has been implicated in the pathogenesis of NAFLD. This study was aimed at observing the relationship between insulin resistance and NAFLD, and evaluating the role of pioglitazone (PGZ) acting as insulin-sensitizing agents in the prevention and treatment of rat fatty liver induced by high fat feeding.

METHODS

The rats were separated randomly into 6 groups: model group I were fed high fat diet for 8 weeks, PGZ prevention group were given PGZ 4 mg/(kg.d) simultaneously, while control group I were fed normal food for 8 weeks; model group II were fed high fat diet for 16 weeks, PGZ treatment group were given PGZ 4 mg/(kg.d) orally simultaneous with high fat diet for 8 weeks after high fat feeding for 8 weeks, control group II were fed normal food for 16 weeks. The rats were sacrificed after 8 weeks and 16 weeks respectively. Liver weight, body weight, serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), tumor necrosis factor alpha (TNF-alpha), fasting blood glucose (FBG), fasting plasma insulin (FINS), HOMA (homeostasis model assessment) insulin resistance index (HOMA-IR), and the liver histology of rats of all groups were assayed.

RESULTS

After 8 weeks, the liver in model group I showed typical steatosis, accompanied with mild to moderate lobular inflammatory cell infiltration, liver indexes and serum levels of ALT, AST, ALP, TNF-alpha were significantly increased (P<0.05) compared with control group I. Whereas, the degree of hepatic injury was attenuated in PGZ prevention group, liver indexes and serum levels of ALT, ALP were significantly decreased (P<0.05) compared with model group I. After 16 weeks, notable steatosis, and lobular inflammation were observed in model group II rat liver, while the degree of hepatic injury was attenuated in the PGZ treatment group. Liver index, serum levels of ALT, AST, ALP, FINS and HOMA-IR were significantly increased (P<0.05) in model group II compared with control group II. Whereas, in PGZ treatment group, serum levels of AST and FINS showed decreasing tendency, liver indexes, serum levels of ALT, ALP, TNF-alpha and HOMA-IR were significantly decreased compared with model group II.

CONCLUSION

Insulin resistance plays a role in the pathogenesis of NAFLD in rats. Pioglitazone can attenuate insulin resistance and biochemical and histological injury in high fat-induced fatty liver in rats.

Authors+Show Affiliations

Department of Gastroenterology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16845716

Citation

Xu, Ping, et al. "Research On the Protection Effect of Pioglitazone for Non-alcoholic Fatty Liver Disease (NAFLD) in Rats." Journal of Zhejiang University. Science. B, vol. 7, no. 8, 2006, pp. 627-33.
Xu P, Zhang XG, Li YM, et al. Research on the protection effect of pioglitazone for non-alcoholic fatty liver disease (NAFLD) in rats. J Zhejiang Univ Sci B. 2006;7(8):627-33.
Xu, P., Zhang, X. G., Li, Y. M., Yu, C. H., Xu, L., & Xu, G. Y. (2006). Research on the protection effect of pioglitazone for non-alcoholic fatty liver disease (NAFLD) in rats. Journal of Zhejiang University. Science. B, 7(8), 627-33.
Xu P, et al. Research On the Protection Effect of Pioglitazone for Non-alcoholic Fatty Liver Disease (NAFLD) in Rats. J Zhejiang Univ Sci B. 2006;7(8):627-33. PubMed PMID: 16845716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Research on the protection effect of pioglitazone for non-alcoholic fatty liver disease (NAFLD) in rats. AU - Xu,Ping, AU - Zhang,Xing-guo, AU - Li,You-ming, AU - Yu,Chao-hui, AU - Xu,Lei, AU - Xu,Gen-yun, PY - 2006/7/18/pubmed PY - 2006/9/22/medline PY - 2006/7/18/entrez SP - 627 EP - 33 JF - Journal of Zhejiang University. Science. B JO - J Zhejiang Univ Sci B VL - 7 IS - 8 N2 - OBJECTIVE: The prevalence of non-alcoholic fatty liver disease (NAFLD) has markedly increased. Insulin resistance has been implicated in the pathogenesis of NAFLD. This study was aimed at observing the relationship between insulin resistance and NAFLD, and evaluating the role of pioglitazone (PGZ) acting as insulin-sensitizing agents in the prevention and treatment of rat fatty liver induced by high fat feeding. METHODS: The rats were separated randomly into 6 groups: model group I were fed high fat diet for 8 weeks, PGZ prevention group were given PGZ 4 mg/(kg.d) simultaneously, while control group I were fed normal food for 8 weeks; model group II were fed high fat diet for 16 weeks, PGZ treatment group were given PGZ 4 mg/(kg.d) orally simultaneous with high fat diet for 8 weeks after high fat feeding for 8 weeks, control group II were fed normal food for 16 weeks. The rats were sacrificed after 8 weeks and 16 weeks respectively. Liver weight, body weight, serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), tumor necrosis factor alpha (TNF-alpha), fasting blood glucose (FBG), fasting plasma insulin (FINS), HOMA (homeostasis model assessment) insulin resistance index (HOMA-IR), and the liver histology of rats of all groups were assayed. RESULTS: After 8 weeks, the liver in model group I showed typical steatosis, accompanied with mild to moderate lobular inflammatory cell infiltration, liver indexes and serum levels of ALT, AST, ALP, TNF-alpha were significantly increased (P<0.05) compared with control group I. Whereas, the degree of hepatic injury was attenuated in PGZ prevention group, liver indexes and serum levels of ALT, ALP were significantly decreased (P<0.05) compared with model group I. After 16 weeks, notable steatosis, and lobular inflammation were observed in model group II rat liver, while the degree of hepatic injury was attenuated in the PGZ treatment group. Liver index, serum levels of ALT, AST, ALP, FINS and HOMA-IR were significantly increased (P<0.05) in model group II compared with control group II. Whereas, in PGZ treatment group, serum levels of AST and FINS showed decreasing tendency, liver indexes, serum levels of ALT, ALP, TNF-alpha and HOMA-IR were significantly decreased compared with model group II. CONCLUSION: Insulin resistance plays a role in the pathogenesis of NAFLD in rats. Pioglitazone can attenuate insulin resistance and biochemical and histological injury in high fat-induced fatty liver in rats. SN - 1673-1581 UR - https://www.unboundmedicine.com/medline/citation/16845716/Research_on_the_protection_effect_of_pioglitazone_for_non_alcoholic_fatty_liver_disease__NAFLD__in_rats_ L2 - http://www.jzus.zju.edu.cn/article.php?doi=10.1631/jzus.2006.B0627 DB - PRIME DP - Unbound Medicine ER -