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Receptor sub-types involved in responses of Purkinje cell to exogenous excitatory amino acids and local electrical stimulation in cerebellar slices in the rat.
Neuropharmacology. 1991 Oct; 30(10):1029-37.N

Abstract

The effects of the NMDA receptor antagonist, 2-amino-5-phosphonovalerate (APV) and non-NMDA receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) on responses of Purkinje cells to exogenous excitatory amino acids and to electrical stimulation of the parallel fibres, were investigated in slices of the cerebellum of the rat. Glutamate, aspartate, kainate and quisqualate all induced excitation of Purkinje cells. Responses to kainate and quisqualate were blocked by CNQX (10 microM) but not by APV (10 microM). N-Methyl-D-aspartate induced biphasic excitatory-inhibitory responses, both components of which were blocked by APV but not by CNQX. The inhibitory component was less sensitive to blockade by APV but was totally blocked by bicuculline, the GABAA receptor antagonist. Parallel fibre stimulation most commonly induced inhibition of Purkinje cells, with or without preceding excitation. This inhibition was blocked by APV and excitatory responses were often revealed. A less commonly-observed predominantly excitatory response was blocked by CNQX but not by APV and inhibition tended to be revealed. These data suggest that parallel fibre-Purkinje cell synapses possess non-NMDA postsynaptic receptors, while the parallel fibre-inhibitory interneuron synapses possess functional NMDA receptors.

Authors+Show Affiliations

Department of Physiology and Pharmacology, University of Southampton, U.K.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1684644

Citation

Hussain, S, et al. "Receptor Sub-types Involved in Responses of Purkinje Cell to Exogenous Excitatory Amino Acids and Local Electrical Stimulation in Cerebellar Slices in the Rat." Neuropharmacology, vol. 30, no. 10, 1991, pp. 1029-37.
Hussain S, Gardner CR, Bagust J, et al. Receptor sub-types involved in responses of Purkinje cell to exogenous excitatory amino acids and local electrical stimulation in cerebellar slices in the rat. Neuropharmacology. 1991;30(10):1029-37.
Hussain, S., Gardner, C. R., Bagust, J., & Walker, R. J. (1991). Receptor sub-types involved in responses of Purkinje cell to exogenous excitatory amino acids and local electrical stimulation in cerebellar slices in the rat. Neuropharmacology, 30(10), 1029-37.
Hussain S, et al. Receptor Sub-types Involved in Responses of Purkinje Cell to Exogenous Excitatory Amino Acids and Local Electrical Stimulation in Cerebellar Slices in the Rat. Neuropharmacology. 1991;30(10):1029-37. PubMed PMID: 1684644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Receptor sub-types involved in responses of Purkinje cell to exogenous excitatory amino acids and local electrical stimulation in cerebellar slices in the rat. AU - Hussain,S, AU - Gardner,C R, AU - Bagust,J, AU - Walker,R J, PY - 1991/10/1/pubmed PY - 1991/10/1/medline PY - 1991/10/1/entrez SP - 1029 EP - 37 JF - Neuropharmacology JO - Neuropharmacology VL - 30 IS - 10 N2 - The effects of the NMDA receptor antagonist, 2-amino-5-phosphonovalerate (APV) and non-NMDA receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) on responses of Purkinje cells to exogenous excitatory amino acids and to electrical stimulation of the parallel fibres, were investigated in slices of the cerebellum of the rat. Glutamate, aspartate, kainate and quisqualate all induced excitation of Purkinje cells. Responses to kainate and quisqualate were blocked by CNQX (10 microM) but not by APV (10 microM). N-Methyl-D-aspartate induced biphasic excitatory-inhibitory responses, both components of which were blocked by APV but not by CNQX. The inhibitory component was less sensitive to blockade by APV but was totally blocked by bicuculline, the GABAA receptor antagonist. Parallel fibre stimulation most commonly induced inhibition of Purkinje cells, with or without preceding excitation. This inhibition was blocked by APV and excitatory responses were often revealed. A less commonly-observed predominantly excitatory response was blocked by CNQX but not by APV and inhibition tended to be revealed. These data suggest that parallel fibre-Purkinje cell synapses possess non-NMDA postsynaptic receptors, while the parallel fibre-inhibitory interneuron synapses possess functional NMDA receptors. SN - 0028-3908 UR - https://www.unboundmedicine.com/medline/citation/1684644/Receptor_sub_types_involved_in_responses_of_Purkinje_cell_to_exogenous_excitatory_amino_acids_and_local_electrical_stimulation_in_cerebellar_slices_in_the_rat_ DB - PRIME DP - Unbound Medicine ER -