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Evaluation of antioxidant activity of Cassia siamea flowers.
J Ethnopharmacol. 2006 Dec 06; 108(3):340-8.JE

Abstract

The study was aimed at evaluating the antioxidant activity of alcoholic extract of Cassia siamea Lam. (Fabaceae) flowers. The extract was found to contain a large amount of polyphenols and also exhibited an immense reducing ability. At a concentration of 250 microg/ml, 96% of DPPH radicals and at 500 microg/ml, 42.7, 32.7 and 64.5% of O2-, H2O2 and NO respectively could be scavenged by C. siamea flower extract. The extract also inhibited OH radical induced oxidation of protein (BSA) and LPO in murine hepatic microsomes. The determination of metal chelating capacity of the extract indicated chelating of metal ions (Fe2+) to be a putative mechanism implicated in the inhibition of OH radical-induced BSA oxidation and LPO. C. siamea flower extract also exhibited a significant antioxidant activity in acute oxidative tissue injury animal model constituted by CCl4 induced hepatotoxicity. Oral administration of the extract at a dose of 50-150 mg/kg of body weight significantly protected from CCl4 induced elevation in AST and ALT in the serum, elevation in hepatic LPO, depletion of hepatic GSH and decrease in the activities of hepatic antioxidant enzymes: SOD, CAT and GPX. The extract also protected against histopathological changes produced by CCl4 such as necrosis, fatty changes, ballooning degeneration, etc. The data obtained in the present study suggests that the alcoholic extract of C. siamea flowers have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage in the liver.

Authors+Show Affiliations

Department of Medical Elementology & Toxicology, Jamia Hamdard, Hamdard Nagar, New Delhi 110062, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16846707

Citation

Kaur, Gurpreet, et al. "Evaluation of Antioxidant Activity of Cassia Siamea Flowers." Journal of Ethnopharmacology, vol. 108, no. 3, 2006, pp. 340-8.
Kaur G, Alam MS, Jabbar Z, et al. Evaluation of antioxidant activity of Cassia siamea flowers. J Ethnopharmacol. 2006;108(3):340-8.
Kaur, G., Alam, M. S., Jabbar, Z., Javed, K., & Athar, M. (2006). Evaluation of antioxidant activity of Cassia siamea flowers. Journal of Ethnopharmacology, 108(3), 340-8.
Kaur G, et al. Evaluation of Antioxidant Activity of Cassia Siamea Flowers. J Ethnopharmacol. 2006 Dec 6;108(3):340-8. PubMed PMID: 16846707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antioxidant activity of Cassia siamea flowers. AU - Kaur,Gurpreet, AU - Alam,M Sarwar, AU - Jabbar,Zoobi, AU - Javed,Kaleem, AU - Athar,Mohammad, Y1 - 2006/06/12/ PY - 2006/04/24/received PY - 2006/05/12/revised PY - 2006/05/12/accepted PY - 2006/7/19/pubmed PY - 2007/4/17/medline PY - 2006/7/19/entrez SP - 340 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 108 IS - 3 N2 - The study was aimed at evaluating the antioxidant activity of alcoholic extract of Cassia siamea Lam. (Fabaceae) flowers. The extract was found to contain a large amount of polyphenols and also exhibited an immense reducing ability. At a concentration of 250 microg/ml, 96% of DPPH radicals and at 500 microg/ml, 42.7, 32.7 and 64.5% of O2-, H2O2 and NO respectively could be scavenged by C. siamea flower extract. The extract also inhibited OH radical induced oxidation of protein (BSA) and LPO in murine hepatic microsomes. The determination of metal chelating capacity of the extract indicated chelating of metal ions (Fe2+) to be a putative mechanism implicated in the inhibition of OH radical-induced BSA oxidation and LPO. C. siamea flower extract also exhibited a significant antioxidant activity in acute oxidative tissue injury animal model constituted by CCl4 induced hepatotoxicity. Oral administration of the extract at a dose of 50-150 mg/kg of body weight significantly protected from CCl4 induced elevation in AST and ALT in the serum, elevation in hepatic LPO, depletion of hepatic GSH and decrease in the activities of hepatic antioxidant enzymes: SOD, CAT and GPX. The extract also protected against histopathological changes produced by CCl4 such as necrosis, fatty changes, ballooning degeneration, etc. The data obtained in the present study suggests that the alcoholic extract of C. siamea flowers have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage in the liver. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/16846707/Evaluation_of_antioxidant_activity_of_Cassia_siamea_flowers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(06)00279-0 DB - PRIME DP - Unbound Medicine ER -