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Characterization of the surface enhanced raman scattering (SERS) of bacteria.
J Phys Chem B. 2005 Jan 13; 109(1):312-20.JP

Abstract

The surface enhanced Raman scattering (SERS) of a number of species and strains of bacteria obtained on novel gold nanoparticle (approximately 80 nm) covered SiO(2) substrates excited at 785 nm is reported. Raman cross-section enhancements of >10(4) per bacterium are found for both Gram-positive and Gram-negative bacteria on these SERS active substrates. The SERS spectra of bacteria are spectrally less congested and exhibit greater species differentiation than their corresponding non-SERS (bulk) Raman spectra at this excitation wavelength. Fluorescence observed in the bulk Raman emission of Bacillus species is not apparent in the corresponding SERS spectra. Despite the field enhancement effects arising from the nanostructured metal surface, this fluorescence component appears "quenched" due to an energy transfer process which does not diminish the Raman emission. The surface enhancement effect allows the observation of Raman spectra of single bacterial cells excited at low incident powers and short data acquisition times. SERS spectra of B. anthracis Sterne illustrate this single cell level capability. Comparison with previous SERS studies reveals how the SERS vibrational signatures are strongly dependent on the morphology and nature of the SERS active substrates. The potential of SERS for detection and identification of bacterial pathogens with species and strain specificity on these gold particle covered glassy substrates is demonstrated by these results.

Authors+Show Affiliations

The Photonics Center, Boston University, Boston, Massachusetts 02215, USA. ranjith@bu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16851017

Citation

Premasiri, W R., et al. "Characterization of the Surface Enhanced Raman Scattering (SERS) of Bacteria." The Journal of Physical Chemistry. B, vol. 109, no. 1, 2005, pp. 312-20.
Premasiri WR, Moir DT, Klempner MS, et al. Characterization of the surface enhanced raman scattering (SERS) of bacteria. J Phys Chem B. 2005;109(1):312-20.
Premasiri, W. R., Moir, D. T., Klempner, M. S., Krieger, N., Jones, G., & Ziegler, L. D. (2005). Characterization of the surface enhanced raman scattering (SERS) of bacteria. The Journal of Physical Chemistry. B, 109(1), 312-20.
Premasiri WR, et al. Characterization of the Surface Enhanced Raman Scattering (SERS) of Bacteria. J Phys Chem B. 2005 Jan 13;109(1):312-20. PubMed PMID: 16851017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the surface enhanced raman scattering (SERS) of bacteria. AU - Premasiri,W R, AU - Moir,D T, AU - Klempner,M S, AU - Krieger,N, AU - Jones,G,2nd AU - Ziegler,L D, PY - 2006/7/21/pubmed PY - 2007/6/2/medline PY - 2006/7/21/entrez SP - 312 EP - 20 JF - The journal of physical chemistry. B JO - J Phys Chem B VL - 109 IS - 1 N2 - The surface enhanced Raman scattering (SERS) of a number of species and strains of bacteria obtained on novel gold nanoparticle (approximately 80 nm) covered SiO(2) substrates excited at 785 nm is reported. Raman cross-section enhancements of >10(4) per bacterium are found for both Gram-positive and Gram-negative bacteria on these SERS active substrates. The SERS spectra of bacteria are spectrally less congested and exhibit greater species differentiation than their corresponding non-SERS (bulk) Raman spectra at this excitation wavelength. Fluorescence observed in the bulk Raman emission of Bacillus species is not apparent in the corresponding SERS spectra. Despite the field enhancement effects arising from the nanostructured metal surface, this fluorescence component appears "quenched" due to an energy transfer process which does not diminish the Raman emission. The surface enhancement effect allows the observation of Raman spectra of single bacterial cells excited at low incident powers and short data acquisition times. SERS spectra of B. anthracis Sterne illustrate this single cell level capability. Comparison with previous SERS studies reveals how the SERS vibrational signatures are strongly dependent on the morphology and nature of the SERS active substrates. The potential of SERS for detection and identification of bacterial pathogens with species and strain specificity on these gold particle covered glassy substrates is demonstrated by these results. SN - 1520-6106 UR - https://www.unboundmedicine.com/medline/citation/16851017/Characterization_of_the_surface_enhanced_raman_scattering__SERS__of_bacteria_ L2 - https://doi.org/10.1021/jp040442n DB - PRIME DP - Unbound Medicine ER -