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Excitation wavelength dependence of the proton-transfer reaction of the green fluorescent protein.
J Phys Chem B. 2005 Mar 10; 109(9):4241-51.JP

Abstract

Picosecond time-correlated single-photon counting was used to measure the proton-transfer rate of green fluorescent protein (GFP) excited by several wavelengths between 266 and 405 nm. When samples of GFP in water and D2O are excited at short wavelengths, lambda(ex) < 295 nm, the fluorescence properties are largely modified with respect to excitation at a wavelength around 400 nm, the peak of the absorption band of the S0 --> S1 transition of the ROH form of the chromophore. The shorter the excitation wavelength, the longer the decay time of the ROH emission band at 450 nm and the longer the rise time of the RO- emission band at 512 nm. The proton transfer is slower by an order of magnitude and about a factor of 3 when GFP in water and D2O are excited by 266 nm, respectively.

Authors+Show Affiliations

Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv 69978, Israel. huppert@tulip.tau.ac.ilNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16851487

Citation

Huppert, Dan, et al. "Excitation Wavelength Dependence of the Proton-transfer Reaction of the Green Fluorescent Protein." The Journal of Physical Chemistry. B, vol. 109, no. 9, 2005, pp. 4241-51.
Huppert D, Leiderman P, Ben-Ziv M, et al. Excitation wavelength dependence of the proton-transfer reaction of the green fluorescent protein. J Phys Chem B. 2005;109(9):4241-51.
Huppert, D., Leiderman, P., Ben-Ziv, M., Genosar, L., & Cohen, L. (2005). Excitation wavelength dependence of the proton-transfer reaction of the green fluorescent protein. The Journal of Physical Chemistry. B, 109(9), 4241-51.
Huppert D, et al. Excitation Wavelength Dependence of the Proton-transfer Reaction of the Green Fluorescent Protein. J Phys Chem B. 2005 Mar 10;109(9):4241-51. PubMed PMID: 16851487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Excitation wavelength dependence of the proton-transfer reaction of the green fluorescent protein. AU - Huppert,Dan, AU - Leiderman,Pavel, AU - Ben-Ziv,Moran, AU - Genosar,Liat, AU - Cohen,Lior, PY - 2006/7/21/pubmed PY - 2007/6/1/medline PY - 2006/7/21/entrez SP - 4241 EP - 51 JF - The journal of physical chemistry. B JO - J Phys Chem B VL - 109 IS - 9 N2 - Picosecond time-correlated single-photon counting was used to measure the proton-transfer rate of green fluorescent protein (GFP) excited by several wavelengths between 266 and 405 nm. When samples of GFP in water and D2O are excited at short wavelengths, lambda(ex) < 295 nm, the fluorescence properties are largely modified with respect to excitation at a wavelength around 400 nm, the peak of the absorption band of the S0 --> S1 transition of the ROH form of the chromophore. The shorter the excitation wavelength, the longer the decay time of the ROH emission band at 450 nm and the longer the rise time of the RO- emission band at 512 nm. The proton transfer is slower by an order of magnitude and about a factor of 3 when GFP in water and D2O are excited by 266 nm, respectively. SN - 1520-6106 UR - https://www.unboundmedicine.com/medline/citation/16851487/Excitation_wavelength_dependence_of_the_proton_transfer_reaction_of_the_green_fluorescent_protein_ DB - PRIME DP - Unbound Medicine ER -