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[Effects of lecithin-like oxidized low-density lipoprotein receptor-1 on p38 mitogen-activated protein kinase induced by oxidized low-density lipoprotein in endothelial cells].
Zhonghua Yi Xue Za Zhi. 2006 Jun 27; 86(24):1697-700.ZY

Abstract

OBJECTIVE

To investigate the effects of lecithin-like oxidized low-density lipoprotein receptor-1 (LOX-1) on the p38 mitogen-activated protein kinase (p38MAPK) induced by oxidized low-density lipoprotein (ox-LDL) in endothelial cells.

METHODS

Human umbilical vein endothelial cells of the line ECV304 were cultured and treated with LDL 25 microg/ml and ox-LDL of 3 different concentrations (25 microg/ml, 50 microg/ml, and 100 microg/ml) for 24 hours. ECV304 cells without treatment of ox-LDL were used as control group. MTT method was used to detect the optical density (OD) of different groups. RT-PCR was used to detect the mRNA expression of LOX-1. Western blotting was used to detect the protein expression of LOX-1. Another ECV304 cells were randomly divided into 7 groups to be treated with LDL (25 microg/ml), ox-LDL of the concentrations of 25 microg/ml, 50 microg/ml, and 100 microg/ml, ox-LDL 100 microg/ml + JTX92 (LOX-1 blocking antibody) 10 microg/ml, or JTX92 10 microg/ml, or not to be treated with LOX-1 and/or JTX92 (as control group). The p38MAPK protein expression and phosphorylated p38MAPK (p-p38MAPK) protein expression were detected by Western blotting.

RESULTS

The A values of the ECV304 cells treated with ox-LDL were significantly lower than that of the control group dose-dependently (all P < 0.05), however, the A value of the LDL group was not significantly different from that of the control group (P > 0.05). The LOX-1 mRNA expression and protein expression were significantly increased in the ox-LDL groups dose-dependently (both P < 0.01). However, the LOX-1 mRNA expression and protein expression of the LDL group were not significantly different from those of the control group (both P > 0.05). The p38MAPK protein expression level was not significantly different among different groups (all P > 0.05). The p-p38MAPK protein expression was up-regulated by ox-LDL dose-dependently (P < 0.05). The p-p38MAPK protein level of the ox-LDL + JTX92 group was significantly lower than that of the ox-LDL group (P < 0.05), However, the p-p38MAPK protein expression of the JTX92 group was not significantly different from that of the control group.

CONCLUSION

ox-LDL activates the p38MAPK signal pathway through its receptor LOX-1. Upregulation of LOX-1 is an important ring causing atherosclerosis.

Authors+Show Affiliations

Department of Cardiology, First Affiliated Hospital of China Medical University, Shenyang 110001, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16854325

Citation

Hao, Wen-jun, et al. "[Effects of Lecithin-like Oxidized Low-density Lipoprotein Receptor-1 On P38 Mitogen-activated Protein Kinase Induced By Oxidized Low-density Lipoprotein in Endothelial Cells]." Zhonghua Yi Xue Za Zhi, vol. 86, no. 24, 2006, pp. 1697-700.
Hao WJ, Bai XJ, Yang XH. [Effects of lecithin-like oxidized low-density lipoprotein receptor-1 on p38 mitogen-activated protein kinase induced by oxidized low-density lipoprotein in endothelial cells]. Zhonghua Yi Xue Za Zhi. 2006;86(24):1697-700.
Hao, W. J., Bai, X. J., & Yang, X. H. (2006). [Effects of lecithin-like oxidized low-density lipoprotein receptor-1 on p38 mitogen-activated protein kinase induced by oxidized low-density lipoprotein in endothelial cells]. Zhonghua Yi Xue Za Zhi, 86(24), 1697-700.
Hao WJ, Bai XJ, Yang XH. [Effects of Lecithin-like Oxidized Low-density Lipoprotein Receptor-1 On P38 Mitogen-activated Protein Kinase Induced By Oxidized Low-density Lipoprotein in Endothelial Cells]. Zhonghua Yi Xue Za Zhi. 2006 Jun 27;86(24):1697-700. PubMed PMID: 16854325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of lecithin-like oxidized low-density lipoprotein receptor-1 on p38 mitogen-activated protein kinase induced by oxidized low-density lipoprotein in endothelial cells]. AU - Hao,Wen-jun, AU - Bai,Xiao-juan, AU - Yang,Xiang-hong, PY - 2006/7/21/pubmed PY - 2008/3/5/medline PY - 2006/7/21/entrez SP - 1697 EP - 700 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 86 IS - 24 N2 - OBJECTIVE: To investigate the effects of lecithin-like oxidized low-density lipoprotein receptor-1 (LOX-1) on the p38 mitogen-activated protein kinase (p38MAPK) induced by oxidized low-density lipoprotein (ox-LDL) in endothelial cells. METHODS: Human umbilical vein endothelial cells of the line ECV304 were cultured and treated with LDL 25 microg/ml and ox-LDL of 3 different concentrations (25 microg/ml, 50 microg/ml, and 100 microg/ml) for 24 hours. ECV304 cells without treatment of ox-LDL were used as control group. MTT method was used to detect the optical density (OD) of different groups. RT-PCR was used to detect the mRNA expression of LOX-1. Western blotting was used to detect the protein expression of LOX-1. Another ECV304 cells were randomly divided into 7 groups to be treated with LDL (25 microg/ml), ox-LDL of the concentrations of 25 microg/ml, 50 microg/ml, and 100 microg/ml, ox-LDL 100 microg/ml + JTX92 (LOX-1 blocking antibody) 10 microg/ml, or JTX92 10 microg/ml, or not to be treated with LOX-1 and/or JTX92 (as control group). The p38MAPK protein expression and phosphorylated p38MAPK (p-p38MAPK) protein expression were detected by Western blotting. RESULTS: The A values of the ECV304 cells treated with ox-LDL were significantly lower than that of the control group dose-dependently (all P < 0.05), however, the A value of the LDL group was not significantly different from that of the control group (P > 0.05). The LOX-1 mRNA expression and protein expression were significantly increased in the ox-LDL groups dose-dependently (both P < 0.01). However, the LOX-1 mRNA expression and protein expression of the LDL group were not significantly different from those of the control group (both P > 0.05). The p38MAPK protein expression level was not significantly different among different groups (all P > 0.05). The p-p38MAPK protein expression was up-regulated by ox-LDL dose-dependently (P < 0.05). The p-p38MAPK protein level of the ox-LDL + JTX92 group was significantly lower than that of the ox-LDL group (P < 0.05), However, the p-p38MAPK protein expression of the JTX92 group was not significantly different from that of the control group. CONCLUSION: ox-LDL activates the p38MAPK signal pathway through its receptor LOX-1. Upregulation of LOX-1 is an important ring causing atherosclerosis. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/16854325/[Effects_of_lecithin_like_oxidized_low_density_lipoprotein_receptor_1_on_p38_mitogen_activated_protein_kinase_induced_by_oxidized_low_density_lipoprotein_in_endothelial_cells]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2006&amp;vol=86&amp;issue=24&amp;fpage=1697 DB - PRIME DP - Unbound Medicine ER -