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Antibiotic resistance of Salmonella enterica serovar Paratyphi A in India: emerging and reemerging problem.
J Postgrad Med. 2006 Jul-Sep; 52(3):163-6.JP

Abstract

BACKGROUND

Antibiotic resistance pattern and R-plasmid of Salmonella enterica serovar Paratyphi A isolates from Kolkata, India are not well documented.

AIMS

To determine the trend of antibiotic resistance of S. paratyphi A isolates.

SETTINGS AND DESIGN

A retrospective study was carried out using blood culture isolates of S. paratyphi A (1991 to 2005) obtained from patients of enteric fever from Asansol and Kolkata and its suburbs (India).

MATERIALS AND METHODS

Antibiotic susceptibility pattern, using seven antibiotics, for the isolates was determined following agar dilution and disk diffusion methods. Transferability of multidrug resistance to ampicillin (Am), chloramphenicol (Chl), cotrimoxazole (Cot) and tetracycline (Tet) among the isolates was determined by in vitro conjugation. The multi-drug resistant (MDR) and antibiotic susceptible S. paratyphi A strains and the trans-conjugants were screened for the presence of plasmid.

STATISTICAL ANALYSIS USED

The t test was used to compare the difference between mean minimum inhibitory concentration values of ciprofloxacin (Cp) for nalidixic acid (Nx)-resistant and Nalidixic acid (Nx)-susceptible isolates.

RESULTS

Among 13 outbreak causing isolates in 1991, 9 (69.23%) showed AmChlCotTet-resistance, while 4 (30.77%) Cot-resistance only. During 1992-1994, all 13 isolates were susceptible to Am, Chl, Cot and Tet. During 1995-2005, isolates demonstrated different resistance patterns and emergence of nalidixic acid (Nx)-resistance. A transferable plasmid conferring AmChlCotTet-resistance was detected among MDR isolates. All the isolates were susceptible to ceftriaxone (Ctx) and ciprofloxacin (Cp). Association between Nalidixic acid (Nx)-resistance and reduced susceptibility to ciprofloxacin (Cp) among 59 S. paratyphi A isolates was noticed (P < 0.001).

CONCLUSION

Vigilance for R-plasmid and surveillance of antibiotic susceptibility among S. paratyphi A isolates in and around Kolkata, India, are mandatory in order to combat antibiotic resistance of the isolates in this part of the world.

Authors+Show Affiliations

Department of Bacteriology and Serology, Calcutta School of Tropical Medicine, C. R. Avenue, Kolkata 700073, India. samtropmed@rediffmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16855314

Citation

Mandal, Shyamapada, et al. "Antibiotic Resistance of Salmonella Enterica Serovar Paratyphi a in India: Emerging and Reemerging Problem." Journal of Postgraduate Medicine, vol. 52, no. 3, 2006, pp. 163-6.
Mandal S, Mandal MD, Pal NK. Antibiotic resistance of Salmonella enterica serovar Paratyphi A in India: emerging and reemerging problem. J Postgrad Med. 2006;52(3):163-6.
Mandal, S., Mandal, M. D., & Pal, N. K. (2006). Antibiotic resistance of Salmonella enterica serovar Paratyphi A in India: emerging and reemerging problem. Journal of Postgraduate Medicine, 52(3), 163-6.
Mandal S, Mandal MD, Pal NK. Antibiotic Resistance of Salmonella Enterica Serovar Paratyphi a in India: Emerging and Reemerging Problem. J Postgrad Med. 2006 Jul-Sep;52(3):163-6. PubMed PMID: 16855314.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibiotic resistance of Salmonella enterica serovar Paratyphi A in India: emerging and reemerging problem. AU - Mandal,Shyamapada, AU - Mandal,M D, AU - Pal,N K, PY - 2006/7/21/pubmed PY - 2007/2/21/medline PY - 2006/7/21/entrez SP - 163 EP - 6 JF - Journal of postgraduate medicine JO - J Postgrad Med VL - 52 IS - 3 N2 - BACKGROUND: Antibiotic resistance pattern and R-plasmid of Salmonella enterica serovar Paratyphi A isolates from Kolkata, India are not well documented. AIMS: To determine the trend of antibiotic resistance of S. paratyphi A isolates. SETTINGS AND DESIGN: A retrospective study was carried out using blood culture isolates of S. paratyphi A (1991 to 2005) obtained from patients of enteric fever from Asansol and Kolkata and its suburbs (India). MATERIALS AND METHODS: Antibiotic susceptibility pattern, using seven antibiotics, for the isolates was determined following agar dilution and disk diffusion methods. Transferability of multidrug resistance to ampicillin (Am), chloramphenicol (Chl), cotrimoxazole (Cot) and tetracycline (Tet) among the isolates was determined by in vitro conjugation. The multi-drug resistant (MDR) and antibiotic susceptible S. paratyphi A strains and the trans-conjugants were screened for the presence of plasmid. STATISTICAL ANALYSIS USED: The t test was used to compare the difference between mean minimum inhibitory concentration values of ciprofloxacin (Cp) for nalidixic acid (Nx)-resistant and Nalidixic acid (Nx)-susceptible isolates. RESULTS: Among 13 outbreak causing isolates in 1991, 9 (69.23%) showed AmChlCotTet-resistance, while 4 (30.77%) Cot-resistance only. During 1992-1994, all 13 isolates were susceptible to Am, Chl, Cot and Tet. During 1995-2005, isolates demonstrated different resistance patterns and emergence of nalidixic acid (Nx)-resistance. A transferable plasmid conferring AmChlCotTet-resistance was detected among MDR isolates. All the isolates were susceptible to ceftriaxone (Ctx) and ciprofloxacin (Cp). Association between Nalidixic acid (Nx)-resistance and reduced susceptibility to ciprofloxacin (Cp) among 59 S. paratyphi A isolates was noticed (P < 0.001). CONCLUSION: Vigilance for R-plasmid and surveillance of antibiotic susceptibility among S. paratyphi A isolates in and around Kolkata, India, are mandatory in order to combat antibiotic resistance of the isolates in this part of the world. SN - 0022-3859 UR - https://www.unboundmedicine.com/medline/citation/16855314/Antibiotic_resistance_of_Salmonella_enterica_serovar_Paratyphi_A_in_India:_emerging_and_reemerging_problem_ DB - PRIME DP - Unbound Medicine ER -