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Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel).
Syst Appl Microbiol. 2007 Apr; 30(3):239-54.SA

Abstract

The microbial community structure of an anoxic profundal lake sediment, i.e., subtropical Lake Kinneret, was analysed with respect to its composition by culture-independent molecular methods including terminal restriction fragment length polymorphism (T-RFLP) analysis, comparative sequence analysis, and quantitative real-time PCR. In particular we were interested in the structure, species composition, and relative abundance of the overall microbial community in the methanogenic sediment layer (0-10 cm depth). Pairwise comparison of archaeal and bacterial 16S rRNA gene T-RFLP profiles obtained from three independent samplings indicated stability of the microbial community. The numbers of Archaea and Bacteria, quantified by real-time PCR, amounted to about 10(8) and 10(10) 16S rRNA gene copies cm(-3) sediment, respectively, suggesting that Archaea may account for only a minor fraction (approximately 1%) of the total prokaryotic community. Hydrogenotrophic Methanomicrobiales and acetoclastic Methanosaeta spp. dominated T-RFLP profiles of the archaeal community. T-RFLP profiles of the bacterial community were dominated by Deltaproteobacteria, sulphate reducers and syntrophs in particular. The second most abundant group was assigned to the Bacteroidetes-Chlorobi-group. Only one bacterial group, which was affiliated with halorespiring bacteria of subphylum II of the Chloroflexi, showed variation in abundance within the sediment samples investigated. Our study gives a comprehensive insight into the structure of the bacterial and archaeal community of a profundal lake sediment, indicating that sulphate reducers, syntrophs, bacteroidetes, halorespirers and methanogens are of particular importance in Lake Kinneret sediment.

Authors+Show Affiliations

Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch-Str., 35043 Marburg, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16857336

Citation

Schwarz, Julia I K., et al. "Community Structure of Archaea and Bacteria in a Profundal Lake Sediment Lake Kinneret (Israel)." Systematic and Applied Microbiology, vol. 30, no. 3, 2007, pp. 239-54.
Schwarz JI, Eckert W, Conrad R. Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel). Syst Appl Microbiol. 2007;30(3):239-54.
Schwarz, J. I., Eckert, W., & Conrad, R. (2007). Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel). Systematic and Applied Microbiology, 30(3), 239-54.
Schwarz JI, Eckert W, Conrad R. Community Structure of Archaea and Bacteria in a Profundal Lake Sediment Lake Kinneret (Israel). Syst Appl Microbiol. 2007;30(3):239-54. PubMed PMID: 16857336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Community structure of Archaea and Bacteria in a profundal lake sediment Lake Kinneret (Israel). AU - Schwarz,Julia I K, AU - Eckert,Werner, AU - Conrad,Ralf, Y1 - 2006/07/18/ PY - 2006/04/18/received PY - 2006/05/26/revised PY - 2006/05/30/accepted PY - 2006/7/22/pubmed PY - 2007/8/8/medline PY - 2006/7/22/entrez SP - 239 EP - 54 JF - Systematic and applied microbiology JO - Syst Appl Microbiol VL - 30 IS - 3 N2 - The microbial community structure of an anoxic profundal lake sediment, i.e., subtropical Lake Kinneret, was analysed with respect to its composition by culture-independent molecular methods including terminal restriction fragment length polymorphism (T-RFLP) analysis, comparative sequence analysis, and quantitative real-time PCR. In particular we were interested in the structure, species composition, and relative abundance of the overall microbial community in the methanogenic sediment layer (0-10 cm depth). Pairwise comparison of archaeal and bacterial 16S rRNA gene T-RFLP profiles obtained from three independent samplings indicated stability of the microbial community. The numbers of Archaea and Bacteria, quantified by real-time PCR, amounted to about 10(8) and 10(10) 16S rRNA gene copies cm(-3) sediment, respectively, suggesting that Archaea may account for only a minor fraction (approximately 1%) of the total prokaryotic community. Hydrogenotrophic Methanomicrobiales and acetoclastic Methanosaeta spp. dominated T-RFLP profiles of the archaeal community. T-RFLP profiles of the bacterial community were dominated by Deltaproteobacteria, sulphate reducers and syntrophs in particular. The second most abundant group was assigned to the Bacteroidetes-Chlorobi-group. Only one bacterial group, which was affiliated with halorespiring bacteria of subphylum II of the Chloroflexi, showed variation in abundance within the sediment samples investigated. Our study gives a comprehensive insight into the structure of the bacterial and archaeal community of a profundal lake sediment, indicating that sulphate reducers, syntrophs, bacteroidetes, halorespirers and methanogens are of particular importance in Lake Kinneret sediment. SN - 0723-2020 UR - https://www.unboundmedicine.com/medline/citation/16857336/Community_structure_of_Archaea_and_Bacteria_in_a_profundal_lake_sediment_Lake_Kinneret__Israel__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0723-2020(06)00073-7 DB - PRIME DP - Unbound Medicine ER -