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Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease.
Clin Gastroenterol Hepatol. 2006 Sep; 4(9):1112-7.CG

Abstract

BACKGROUND & AIMS

Our aim was to explore the diagnostic value of a newly developed synthetic peptide antibody assay addressing specific synthetic gliadin-derived deamidated peptides (AGA II) for the diagnosis of celiac disease (CD).

METHODS

We assayed serum samples obtained prospectively at diagnosis from a population of 92 consecutive adult patients with CD and 113 non-CD controls. Patients were reevaluated after 6 months (n = 56) and 1 year (n = 20) of treatment. All patients and controls underwent intestinal biopsy and a set of CD-related serology tests. A newly developed enzyme-linked immunosorbent assay (ELISA) for detecting IgA and IgG antibodies against synthetic deamidated gliadin epitopes was used.

RESULTS

At diagnosis, sensitivity and specificity were 94.6% and 99.1% for AGA II IgA and 92.4% and 100% for AGA II IgG. Absolute values and the proportion of positive samples for both antibodies were significantly reduced at 6 months (P < .0000) and 1 year (P < .001) after initiation of a gluten-free diet. Compared with conventional AGA, the peptide antibodies had greater sensitivity, specificity, positive and negative predictive values, accuracy, and likelihood ratios. Compared with antitissue transglutaminase antibodies, AGA II had similar sensitivity but greater specificity and predictive values, better likelihood ratios, and an excellent agreement (kappa statistic = .92).

CONCLUSIONS

This study assessed the value of an ELISA assay in detecting antibodies to gliadin-related peptides. This assay appears to be a reliable tool for diagnosing CD and suggests promising accuracy that may be very useful in clinical practice.

Authors+Show Affiliations

Small Bowel Diseases Clinic, Division of Pathology, Department of Medicine, Dr. Carlos Bonorino Udaondo Hospital of Gastroenterology, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article

Language

eng

PubMed ID

16860613

Citation

Sugai, Emilia, et al. "Accuracy of Testing for Antibodies to Synthetic Gliadin-related Peptides in Celiac Disease." Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association, vol. 4, no. 9, 2006, pp. 1112-7.
Sugai E, Vázquez H, Nachman F, et al. Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. Clin Gastroenterol Hepatol. 2006;4(9):1112-7.
Sugai, E., Vázquez, H., Nachman, F., Moreno, M. L., Mazure, R., Smecuol, E., Niveloni, S., Cabanne, A., Kogan, Z., Gómez, J. C., Mauriño, E., & Bai, J. C. (2006). Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association, 4(9), 1112-7.
Sugai E, et al. Accuracy of Testing for Antibodies to Synthetic Gliadin-related Peptides in Celiac Disease. Clin Gastroenterol Hepatol. 2006;4(9):1112-7. PubMed PMID: 16860613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accuracy of testing for antibodies to synthetic gliadin-related peptides in celiac disease. AU - Sugai,Emilia, AU - Vázquez,Horacio, AU - Nachman,Fabio, AU - Moreno,María Laura, AU - Mazure,Roberto, AU - Smecuol,Edgardo, AU - Niveloni,Sonia, AU - Cabanne,Ana, AU - Kogan,Zulema, AU - Gómez,Juan C, AU - Mauriño,Eduardo, AU - Bai,Julio C, Y1 - 2006/07/24/ PY - 2006/7/25/pubmed PY - 2006/12/9/medline PY - 2006/7/25/entrez SP - 1112 EP - 7 JF - Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association JO - Clin Gastroenterol Hepatol VL - 4 IS - 9 N2 - BACKGROUND & AIMS: Our aim was to explore the diagnostic value of a newly developed synthetic peptide antibody assay addressing specific synthetic gliadin-derived deamidated peptides (AGA II) for the diagnosis of celiac disease (CD). METHODS: We assayed serum samples obtained prospectively at diagnosis from a population of 92 consecutive adult patients with CD and 113 non-CD controls. Patients were reevaluated after 6 months (n = 56) and 1 year (n = 20) of treatment. All patients and controls underwent intestinal biopsy and a set of CD-related serology tests. A newly developed enzyme-linked immunosorbent assay (ELISA) for detecting IgA and IgG antibodies against synthetic deamidated gliadin epitopes was used. RESULTS: At diagnosis, sensitivity and specificity were 94.6% and 99.1% for AGA II IgA and 92.4% and 100% for AGA II IgG. Absolute values and the proportion of positive samples for both antibodies were significantly reduced at 6 months (P < .0000) and 1 year (P < .001) after initiation of a gluten-free diet. Compared with conventional AGA, the peptide antibodies had greater sensitivity, specificity, positive and negative predictive values, accuracy, and likelihood ratios. Compared with antitissue transglutaminase antibodies, AGA II had similar sensitivity but greater specificity and predictive values, better likelihood ratios, and an excellent agreement (kappa statistic = .92). CONCLUSIONS: This study assessed the value of an ELISA assay in detecting antibodies to gliadin-related peptides. This assay appears to be a reliable tool for diagnosing CD and suggests promising accuracy that may be very useful in clinical practice. SN - 1542-3565 UR - https://www.unboundmedicine.com/medline/citation/16860613/Accuracy_of_testing_for_antibodies_to_synthetic_gliadin_related_peptides_in_celiac_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1542-3565(06)00469-1 DB - PRIME DP - Unbound Medicine ER -