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The role of Yap1p and Skn7p-mediated oxidative stress response in the defence of Saccharomyces cerevisiae against singlet oxygen.
Yeast. 2006 Jul 30; 23(10):741-50.Y

Abstract

The production of the reactive oxygen species superoxide and hydrogen peroxide in Saccharomyces cerevisiae induces the expression of various defence genes involved in an oxidative stress response. Expression of many of these genes has been shown to be coordinated by two transcriptional regulators, Yap1p and Skn7p, either alone or in concert. Here, we investigated the role of the Yap1p and Skn7p-mediated stress response in the defence against singlet oxygen, a non-radical reactive oxygen species produced mainly by photosensitized reactions in illuminated cells. Both, a yap1 and skn7 mutant were highly sensitive to Rose Bengal, an exogenous photosensitizer producing singlet oxygen in the light. The expression of a Yap1p-dependent reporter gene was induced by increased singlet oxygen production, showing that singlet oxygen activates general oxidative stress response mechanisms required for the resistance against Rose Bengal treatment. This response was also slightly stimulated by light in the absence of the photosensitizer, possibly due to singlet oxygen production by endogenous photosensitizers. The expression pattern of four oxidative stress genes in a yap1, skn7 and wild-type strain and the sensitivity of the corresponding mutants exposed to different oxidative stress conditions proved a role of Yap1p and Skn7p in the defence against singlet oxygen. Similarities in the genetic responses against singlet oxygen and hydroperoxides suggest an overlap in the oxidative stress response against these reactive oxygen species.

Authors+Show Affiliations

Brombacher K
Eawag, Swiss Federal Institute of Aquatic Science and Technology, Department of Environmental Toxicology, Ueberlandstrasse 133, 8600 Dübendorf, Switzerland.
Fischer BB
No affiliation info available
Rüfenacht K
No affiliation info available
Eggen RI
No affiliation info available

MeSH

DNA-Binding ProteinsGene Expression Regulation, EnzymologicGene Expression Regulation, FungalGlutamate-Cysteine LigaseHydrogen PeroxideMembrane ProteinsOxidative StressPeroxidasesPhotosensitizing AgentsRNA, FungalReverse Transcriptase Polymerase Chain ReactionRose BengalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSinglet OxygenThioredoxin-Disulfide ReductaseThioredoxinsTranscription FactorsVitamin K 3

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16862604

Citation

Brombacher, Katrin, et al. "The Role of Yap1p and Skn7p-mediated Oxidative Stress Response in the Defence of Saccharomyces Cerevisiae Against Singlet Oxygen." Yeast (Chichester, England), vol. 23, no. 10, 2006, pp. 741-50.
Brombacher K, Fischer BB, Rüfenacht K, et al. The role of Yap1p and Skn7p-mediated oxidative stress response in the defence of Saccharomyces cerevisiae against singlet oxygen. Yeast. 2006;23(10):741-50.
Brombacher, K., Fischer, B. B., Rüfenacht, K., & Eggen, R. I. (2006). The role of Yap1p and Skn7p-mediated oxidative stress response in the defence of Saccharomyces cerevisiae against singlet oxygen. Yeast (Chichester, England), 23(10), 741-50.
Brombacher K, et al. The Role of Yap1p and Skn7p-mediated Oxidative Stress Response in the Defence of Saccharomyces Cerevisiae Against Singlet Oxygen. Yeast. 2006 Jul 30;23(10):741-50. PubMed PMID: 16862604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of Yap1p and Skn7p-mediated oxidative stress response in the defence of Saccharomyces cerevisiae against singlet oxygen. AU - Brombacher,Katrin, AU - Fischer,Beat B, AU - Rüfenacht,Karin, AU - Eggen,Rik I L, PY - 2006/7/25/pubmed PY - 2006/10/4/medline PY - 2006/7/25/entrez SP - 741 EP - 50 JF - Yeast (Chichester, England) JO - Yeast VL - 23 IS - 10 N2 - The production of the reactive oxygen species superoxide and hydrogen peroxide in Saccharomyces cerevisiae induces the expression of various defence genes involved in an oxidative stress response. Expression of many of these genes has been shown to be coordinated by two transcriptional regulators, Yap1p and Skn7p, either alone or in concert. Here, we investigated the role of the Yap1p and Skn7p-mediated stress response in the defence against singlet oxygen, a non-radical reactive oxygen species produced mainly by photosensitized reactions in illuminated cells. Both, a yap1 and skn7 mutant were highly sensitive to Rose Bengal, an exogenous photosensitizer producing singlet oxygen in the light. The expression of a Yap1p-dependent reporter gene was induced by increased singlet oxygen production, showing that singlet oxygen activates general oxidative stress response mechanisms required for the resistance against Rose Bengal treatment. This response was also slightly stimulated by light in the absence of the photosensitizer, possibly due to singlet oxygen production by endogenous photosensitizers. The expression pattern of four oxidative stress genes in a yap1, skn7 and wild-type strain and the sensitivity of the corresponding mutants exposed to different oxidative stress conditions proved a role of Yap1p and Skn7p in the defence against singlet oxygen. Similarities in the genetic responses against singlet oxygen and hydroperoxides suggest an overlap in the oxidative stress response against these reactive oxygen species. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/16862604/The_role_of_Yap1p_and_Skn7p_mediated_oxidative_stress_response_in_the_defence_of_Saccharomyces_cerevisiae_against_singlet_oxygen_ L2 - https://doi.org/10.1002/yea.1392 DB - PRIME DP - Unbound Medicine ER -