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[Mechanism of chondrocyte apoptosis in Kashin-Beck disease and primary osteoarthritis: a comparative study].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jul; 26(7):927-30.NF

Abstract

OBJECTIVE

To investigate chondrocyte apoptosis and the expressions of Bcl-2, Bax, Fas and iNOS in the articular cartilage between Kashin-Beck disease (KBD) and primary osteoarthritis (OA) and explore the difference in pathogenesis between the two diseases.

METHODS

The articular cartilage specimens were collected from 15 normal human subjects, 15 adult patients with KBD and 15 with OA. Chondrocyte apoptosis was detected by TUNEL method, and the expressions of Bcl-2, Bax, Fas and iNOS in articular cartilage were examined with B-SA immunohistochemistry.

RESULTS

The percentages of apoptotic chondrocytes positive for TUNEL staining in the articular cartilage were significantly higher in patients with KBD and OA than in normal control subjects (F=20.90-53.16, df=42, P<0.01), and the erosive areas of the articular cartilage contained greater percentage of apoptotic chondrocytes than the non-erosive areas in the same patient with KBD (t=4.154, df=28, P<0.01) or OA (t=6.004, df=28, P<0.01). No significant difference was noted in the positive apoptotic chondrocytes between KBD and OA (t=1.329-1.362, df=28, P>0.05). The percentage of chondrocytes positive for Bcl-2, Bax, Fas and iNOS were significantly higher in KBD and OA patients than in the control subjects (F=25.46-215.31, df=42, P<0.01), and significant differences were observed in Bcl-2, Bax, Fas and iNOS expressions between the erosed areas and non-erosed areas in articular cartilage in patients with KBD (t=2.608-7.77, df=28, P<0.05) and OA (t=2.278-5.413, df=28, P<0.05), but their expressions showed no significant difference between the two diseases (t=0.284-1.590, df=28, P>0.05).

CONCLUSION

There was no significant difference in apoptotic chondrocytes and Bcl-2, Bax, Fas and iNOS expressions in the cartilage between adult patients with KBD and OA.

Authors+Show Affiliations

Department of Pathophysiology, Medical College of Xi'an Jiaotong University, Xi'an 710061, China. sxjaner@21cn.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16864079

Citation

Wang, Shi-jie, et al. "[Mechanism of Chondrocyte Apoptosis in Kashin-Beck Disease and Primary Osteoarthritis: a Comparative Study]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 7, 2006, pp. 927-30.
Wang SJ, Guo X, Chen JH, et al. [Mechanism of chondrocyte apoptosis in Kashin-Beck disease and primary osteoarthritis: a comparative study]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(7):927-30.
Wang, S. J., Guo, X., Chen, J. H., Ren, F. L., Guo, R., Liu, J. J., & Zhang, Z. T. (2006). [Mechanism of chondrocyte apoptosis in Kashin-Beck disease and primary osteoarthritis: a comparative study]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(7), 927-30.
Wang SJ, et al. [Mechanism of Chondrocyte Apoptosis in Kashin-Beck Disease and Primary Osteoarthritis: a Comparative Study]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(7):927-30. PubMed PMID: 16864079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mechanism of chondrocyte apoptosis in Kashin-Beck disease and primary osteoarthritis: a comparative study]. AU - Wang,Shi-jie, AU - Guo,Xiong, AU - Chen,Jing-hong, AU - Ren,Feng-ling, AU - Guo,Rong, AU - Liu,Jin-jun, AU - Zhang,Zeng-tie, PY - 2006/7/26/pubmed PY - 2008/4/25/medline PY - 2006/7/26/entrez SP - 927 EP - 30 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 7 N2 - OBJECTIVE: To investigate chondrocyte apoptosis and the expressions of Bcl-2, Bax, Fas and iNOS in the articular cartilage between Kashin-Beck disease (KBD) and primary osteoarthritis (OA) and explore the difference in pathogenesis between the two diseases. METHODS: The articular cartilage specimens were collected from 15 normal human subjects, 15 adult patients with KBD and 15 with OA. Chondrocyte apoptosis was detected by TUNEL method, and the expressions of Bcl-2, Bax, Fas and iNOS in articular cartilage were examined with B-SA immunohistochemistry. RESULTS: The percentages of apoptotic chondrocytes positive for TUNEL staining in the articular cartilage were significantly higher in patients with KBD and OA than in normal control subjects (F=20.90-53.16, df=42, P<0.01), and the erosive areas of the articular cartilage contained greater percentage of apoptotic chondrocytes than the non-erosive areas in the same patient with KBD (t=4.154, df=28, P<0.01) or OA (t=6.004, df=28, P<0.01). No significant difference was noted in the positive apoptotic chondrocytes between KBD and OA (t=1.329-1.362, df=28, P>0.05). The percentage of chondrocytes positive for Bcl-2, Bax, Fas and iNOS were significantly higher in KBD and OA patients than in the control subjects (F=25.46-215.31, df=42, P<0.01), and significant differences were observed in Bcl-2, Bax, Fas and iNOS expressions between the erosed areas and non-erosed areas in articular cartilage in patients with KBD (t=2.608-7.77, df=28, P<0.05) and OA (t=2.278-5.413, df=28, P<0.05), but their expressions showed no significant difference between the two diseases (t=0.284-1.590, df=28, P>0.05). CONCLUSION: There was no significant difference in apoptotic chondrocytes and Bcl-2, Bax, Fas and iNOS expressions in the cartilage between adult patients with KBD and OA. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/16864079/[Mechanism_of_chondrocyte_apoptosis_in_Kashin_Beck_disease_and_primary_osteoarthritis:_a_comparative_study]_ L2 - https://medlineplus.gov/osteoarthritis.html DB - PRIME DP - Unbound Medicine ER -