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[Cloning and identification of the cDNA and genomic DNA sequences of the defensin gene of anopheles sinensis].

Abstract

OBJECTIVE

To clone and identify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene.

METHODS

Referring to the published defensin gene sequences of Aedes aegypti and Anopheles gambiae, pairs of primers were designed to amplify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene with template of Anopheles sinensis total RNA by RT-PCR and genomic DNA by nested PCR, respectively. These amplified fractions were cloned and sequenced, and were further identified and analyzed by relevant bioinformatics softwares.

RESULTS

Complete genomic DNA sequence was cloned, including 5' and 3' UTR fraction and two exons separated by a 85 bp intron. Besides, the whole cDNA sequence of Anopheles sinensis defensin gene with 324 bp was also cloned, and its ORF encoded 107 amino acids. The mature peptide had 40 amino acids residues.

CONCLUSION

The whole cDNA sequence and complete genomic DNA sequence of the defensin gene of Anopheles sinensis have been cloned and identified for the first time.

Authors+Show Affiliations

Department of Pathogenic Biology, Southern Medical University, Guangzhou 510515, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16866140

Citation

Zhang, Ya-jing, et al. "[Cloning and Identification of the cDNA and Genomic DNA Sequences of the Defensin Gene of Anopheles Sinensis]." Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, vol. 24, no. 1, 2006, pp. 35-40.
Zhang YJ, Chen XG, Zheng XL, et al. [Cloning and identification of the cDNA and genomic DNA sequences of the defensin gene of anopheles sinensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006;24(1):35-40.
Zhang, Y. J., Chen, X. G., Zheng, X. L., & Wang, C. M. (2006). [Cloning and identification of the cDNA and genomic DNA sequences of the defensin gene of anopheles sinensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases, 24(1), pp. 35-40.
Zhang YJ, et al. [Cloning and Identification of the cDNA and Genomic DNA Sequences of the Defensin Gene of Anopheles Sinensis]. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2006 Feb 28;24(1):35-40. PubMed PMID: 16866140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Cloning and identification of the cDNA and genomic DNA sequences of the defensin gene of anopheles sinensis]. AU - Zhang,Ya-jing, AU - Chen,Xiao-guang, AU - Zheng,Xue-li, AU - Wang,Chun-mei, PY - 2006/7/27/pubmed PY - 2009/1/6/medline PY - 2006/7/27/entrez SP - 35 EP - 40 JF - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases JO - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi VL - 24 IS - 1 N2 - OBJECTIVE: To clone and identify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene. METHODS: Referring to the published defensin gene sequences of Aedes aegypti and Anopheles gambiae, pairs of primers were designed to amplify the cDNA sequence and genomic DNA sequence of Anopheles sinensis defensin gene with template of Anopheles sinensis total RNA by RT-PCR and genomic DNA by nested PCR, respectively. These amplified fractions were cloned and sequenced, and were further identified and analyzed by relevant bioinformatics softwares. RESULTS: Complete genomic DNA sequence was cloned, including 5' and 3' UTR fraction and two exons separated by a 85 bp intron. Besides, the whole cDNA sequence of Anopheles sinensis defensin gene with 324 bp was also cloned, and its ORF encoded 107 amino acids. The mature peptide had 40 amino acids residues. CONCLUSION: The whole cDNA sequence and complete genomic DNA sequence of the defensin gene of Anopheles sinensis have been cloned and identified for the first time. SN - 1000-7423 UR - https://www.unboundmedicine.com/medline/citation/16866140/[Cloning_and_identification_of_the_cDNA_and_genomic_DNA_sequences_of_the_defensin_gene_of_anopheles_sinensis]_ DB - PRIME DP - Unbound Medicine ER -