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The antinociceptive activity of Muntingia calabura aqueous extract and the involvement of L-arginine/nitric oxide/cyclic guanosine monophosphate pathway in its observed activity in mice.
Fundam Clin Pharmacol. 2006 Aug; 20(4):365-72.FC

Abstract

The present study was carried out to investigate on the possible involvement of L-arginine/nitric oxide/cyclic guanosine monophosphate (L-arginine/NO/cGMP) pathway in the aqueous extract of Muntingia calabura (AEMC) leaves antinociception in mice assessed by abdominal constriction test. The AEMC, obtained by soaking the dried leaves in distilled water (DH(2)O) (1 : 2; w/v) for 24 h, was prepared in concentrations of 10%, 50% and 100% that were approximately equivalent to doses of 27, 135 and 270 mg/kg, and administered subcutaneously (s.c.) 5 min after pre-treatment (s.c.) of mice with DH(2)O, L-arginine (20 mg/kg), N(G)-monomethyl-L-arginine acetate (L-NMMA; 20 mg/kg), N(G)-nitro-L-arginine methyl esters (L-NAME; 20 mg/kg), methylene blue (MB) (20 mg/kg), respectively. The AEMC was found to exhibit a concentration-dependent antinociception after pre-challenge with DH(2)O. Interestingly, pre-treatment with L-arginine was found to block significantly (P < 0.05) the AEMC antinociception but only at the highest concentration (100%) of AEMC used. On the other hand, pre-treatment with L-NAME was found to significantly (P < 0.05) enhance the low concentration but inhibit the high concentration AEMC antinociception. MB was found to significantly (P < 0.05) enhance AEMC antinociception at all concentrations used. Except for the higher concentration of AEMC used, co-treatment with L-NAME was found to insignificantly and significantly (P < 0.05) reverse the L-arginine effect when given alone or with low concentration AEMC, respectively. In addition, co-treatment with MB significantly (P < 0.05) reversed the L-arginine effect when given alone or with 10% concentration AEMC but failed to affect the activity of the rest of concentrations used. As a conclusion, this study has demonstrated the involvement of L-arginine/NO/cGMP pathway in AEMC antinociception.

Authors+Show Affiliations

School of Biotechnology and Life Sciences, Universiti Industri Selangor, Jalan Zirkon A7/A, Shah Alam, Selangor, Malaysia. shaza8174@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16867020

Citation

Zakaria, Zainul Amiruddin, et al. "The Antinociceptive Activity of Muntingia Calabura Aqueous Extract and the Involvement of L-arginine/nitric Oxide/cyclic Guanosine Monophosphate Pathway in Its Observed Activity in Mice." Fundamental & Clinical Pharmacology, vol. 20, no. 4, 2006, pp. 365-72.
Zakaria ZA, Sulaiman MR, Jais AM, et al. The antinociceptive activity of Muntingia calabura aqueous extract and the involvement of L-arginine/nitric oxide/cyclic guanosine monophosphate pathway in its observed activity in mice. Fundam Clin Pharmacol. 2006;20(4):365-72.
Zakaria, Z. A., Sulaiman, M. R., Jais, A. M., Somchit, M. N., Jayaraman, K. V., Balakhrisnan, G., & Abdullah, F. C. (2006). The antinociceptive activity of Muntingia calabura aqueous extract and the involvement of L-arginine/nitric oxide/cyclic guanosine monophosphate pathway in its observed activity in mice. Fundamental & Clinical Pharmacology, 20(4), 365-72.
Zakaria ZA, et al. The Antinociceptive Activity of Muntingia Calabura Aqueous Extract and the Involvement of L-arginine/nitric Oxide/cyclic Guanosine Monophosphate Pathway in Its Observed Activity in Mice. Fundam Clin Pharmacol. 2006;20(4):365-72. PubMed PMID: 16867020.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The antinociceptive activity of Muntingia calabura aqueous extract and the involvement of L-arginine/nitric oxide/cyclic guanosine monophosphate pathway in its observed activity in mice. AU - Zakaria,Zainul Amiruddin, AU - Sulaiman,Mohd Roslan, AU - Jais,Abdul Manan Mat, AU - Somchit,Muhammad Nazrul, AU - Jayaraman,Kogilla Vani, AU - Balakhrisnan,Ganesh, AU - Abdullah,Fatimah Corazon, PY - 2006/7/27/pubmed PY - 2006/12/9/medline PY - 2006/7/27/entrez SP - 365 EP - 72 JF - Fundamental & clinical pharmacology JO - Fundam Clin Pharmacol VL - 20 IS - 4 N2 - The present study was carried out to investigate on the possible involvement of L-arginine/nitric oxide/cyclic guanosine monophosphate (L-arginine/NO/cGMP) pathway in the aqueous extract of Muntingia calabura (AEMC) leaves antinociception in mice assessed by abdominal constriction test. The AEMC, obtained by soaking the dried leaves in distilled water (DH(2)O) (1 : 2; w/v) for 24 h, was prepared in concentrations of 10%, 50% and 100% that were approximately equivalent to doses of 27, 135 and 270 mg/kg, and administered subcutaneously (s.c.) 5 min after pre-treatment (s.c.) of mice with DH(2)O, L-arginine (20 mg/kg), N(G)-monomethyl-L-arginine acetate (L-NMMA; 20 mg/kg), N(G)-nitro-L-arginine methyl esters (L-NAME; 20 mg/kg), methylene blue (MB) (20 mg/kg), respectively. The AEMC was found to exhibit a concentration-dependent antinociception after pre-challenge with DH(2)O. Interestingly, pre-treatment with L-arginine was found to block significantly (P < 0.05) the AEMC antinociception but only at the highest concentration (100%) of AEMC used. On the other hand, pre-treatment with L-NAME was found to significantly (P < 0.05) enhance the low concentration but inhibit the high concentration AEMC antinociception. MB was found to significantly (P < 0.05) enhance AEMC antinociception at all concentrations used. Except for the higher concentration of AEMC used, co-treatment with L-NAME was found to insignificantly and significantly (P < 0.05) reverse the L-arginine effect when given alone or with low concentration AEMC, respectively. In addition, co-treatment with MB significantly (P < 0.05) reversed the L-arginine effect when given alone or with 10% concentration AEMC but failed to affect the activity of the rest of concentrations used. As a conclusion, this study has demonstrated the involvement of L-arginine/NO/cGMP pathway in AEMC antinociception. SN - 0767-3981 UR - https://www.unboundmedicine.com/medline/citation/16867020/The_antinociceptive_activity_of_Muntingia_calabura_aqueous_extract_and_the_involvement_of_L_arginine/nitric_oxide/cyclic_guanosine_monophosphate_pathway_in_its_observed_activity_in_mice_ L2 - https://doi.org/10.1111/j.1472-8206.2006.00412.x DB - PRIME DP - Unbound Medicine ER -