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Absorption and metabolism of caffeic acid and chlorogenic acid in the small intestine of rats.
Br J Nutr. 2006 Jul; 96(1):39-46.BJ

Abstract

The absorption and metabolism in the small intestine of chlorogenic acid (5-O-caffeoylquinic acid), the main phenolic acid in the human diet, and of caffeic acid were studied in rats in order to determine whether chlorogenic acid is directly absorbed or hydrolysed in the small intestine. Chlorogenic and caffeic acids were perfused into a segment of ileum plus jejunum during 45 min (50 microm, 0.75 ml/min) using an in situ intestinal perfusion rat model with cannulation of the biliary duct, and were quantified together with their metabolites in perfusion effluent, bile and plasma. The net absorption (influent flux minus effluent flux of phenolic acids and their metabolites) accounted for 19.5 % and 8 % of the perfused caffeic and chlorogenic acids, respectively. A minor fraction of the perfused caffeic acid was metabolized in the intestinal wall and secreted back into the gut lumen in the form of ferulic acid (0.5 % of the perfused flux). Part of the chlorogenic acid (1.2 % of the perfused flux) was recovered in the gut effluent as caffeic acid, showing the presence of trace esterase activity in the gut mucosa. No chlorogenic acid was detected in either plasma or bile, and only low amounts of phenolic acids (less than 0.4 %) were secreted in the bile. The present results show that chlorogenic acid is absorbed and hydrolysed in the small intestine. In contrast to numerous flavonoids, absorbed phenolic acids are poorly excreted in the bile or gut lumen. Their bioavailability therefore appears to be governed largely by their uptake into the gut mucosa.

Authors+Show Affiliations

Unité de Nutrition Humaine, Institut National de la Recherche Agronomique, Centre de Clermont-Ferrand/Theix, 63122 Saint Genès-Champanelle, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16869989

Citation

Lafay, Sophie, et al. "Absorption and Metabolism of Caffeic Acid and Chlorogenic Acid in the Small Intestine of Rats." The British Journal of Nutrition, vol. 96, no. 1, 2006, pp. 39-46.
Lafay S, Morand C, Manach C, et al. Absorption and metabolism of caffeic acid and chlorogenic acid in the small intestine of rats. Br J Nutr. 2006;96(1):39-46.
Lafay, S., Morand, C., Manach, C., Besson, C., & Scalbert, A. (2006). Absorption and metabolism of caffeic acid and chlorogenic acid in the small intestine of rats. The British Journal of Nutrition, 96(1), 39-46.
Lafay S, et al. Absorption and Metabolism of Caffeic Acid and Chlorogenic Acid in the Small Intestine of Rats. Br J Nutr. 2006;96(1):39-46. PubMed PMID: 16869989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Absorption and metabolism of caffeic acid and chlorogenic acid in the small intestine of rats. AU - Lafay,Sophie, AU - Morand,Christine, AU - Manach,Claudine, AU - Besson,Catherine, AU - Scalbert,Augustin, PY - 2006/7/28/pubmed PY - 2006/8/23/medline PY - 2006/7/28/entrez SP - 39 EP - 46 JF - The British journal of nutrition JO - Br J Nutr VL - 96 IS - 1 N2 - The absorption and metabolism in the small intestine of chlorogenic acid (5-O-caffeoylquinic acid), the main phenolic acid in the human diet, and of caffeic acid were studied in rats in order to determine whether chlorogenic acid is directly absorbed or hydrolysed in the small intestine. Chlorogenic and caffeic acids were perfused into a segment of ileum plus jejunum during 45 min (50 microm, 0.75 ml/min) using an in situ intestinal perfusion rat model with cannulation of the biliary duct, and were quantified together with their metabolites in perfusion effluent, bile and plasma. The net absorption (influent flux minus effluent flux of phenolic acids and their metabolites) accounted for 19.5 % and 8 % of the perfused caffeic and chlorogenic acids, respectively. A minor fraction of the perfused caffeic acid was metabolized in the intestinal wall and secreted back into the gut lumen in the form of ferulic acid (0.5 % of the perfused flux). Part of the chlorogenic acid (1.2 % of the perfused flux) was recovered in the gut effluent as caffeic acid, showing the presence of trace esterase activity in the gut mucosa. No chlorogenic acid was detected in either plasma or bile, and only low amounts of phenolic acids (less than 0.4 %) were secreted in the bile. The present results show that chlorogenic acid is absorbed and hydrolysed in the small intestine. In contrast to numerous flavonoids, absorbed phenolic acids are poorly excreted in the bile or gut lumen. Their bioavailability therefore appears to be governed largely by their uptake into the gut mucosa. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/16869989/Absorption_and_metabolism_of_caffeic_acid_and_chlorogenic_acid_in_the_small_intestine_of_rats_ L2 - https://www.cambridge.org/core/product/identifier/S0007114506001759/type/journal_article DB - PRIME DP - Unbound Medicine ER -