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Temporal expression of transforming growth factor-beta2 and myostatin mRNA during embryonic myogenesis in Indian broilers.
Res Vet Sci. 2007 Feb; 82(1):50-3.RV

Abstract

TGF-beta2 and myostatin, the members of TGF family, act through both autocrine and paracrine mechanisms to regulate the growth and differentiation at various developmental stages in chicken. The kinetics and expression profile of these two growth factors were investigated by semi-quantitative RT-PCR, during the myogenesis of Indian broiler chickens. Total RNA was isolated from whole embryos on each of embryonic days (E) 0-6 (n=3 per day) and from the biceps femoris muscle at E7-E18 (n=3 per day). The expression of TGF-beta2 was noticed on E2 that remained at the same level until E6. In biceps femoris muscle, higher level of TGF-beta2 expression was observed during E7-E12, which decreased gradually thereafter. These findings suggested that TGF-beta2 might be a regulatory factor participating in the myogenesis of chicken embryos. Initial myostatin expression was noticed on E1, even before the myogenic lineage is established in embryo. This finding suggested an additional role of myostatin in early chicken embryo development, other than myogenesis. Furthermore, myostatin expression was significantly higher on E3 as compared to earlier studies, where initial higher level was observed at E2, suggesting the differential expression of myostatin among breeds. Higher and almost static myostatin expression was noticed in biceps femoris muscle during the entire period of myogenesis (E7-E18). In the present study, the ontogeny of myostatin expression coincided with myogenesis of chicken. Therefore, it may be hypothesized that myostatin is not only a major determinant of muscle mass, but also involved in early embryogenesis in chickens.

Authors+Show Affiliations

Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute, Avian Genetics, Izatnagar, Bareilly, Uttar Pradesh 243122, India. visheshmeeta@rediffmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16870219

Citation

Saxena, V K., et al. "Temporal Expression of Transforming Growth Factor-beta2 and Myostatin mRNA During Embryonic Myogenesis in Indian Broilers." Research in Veterinary Science, vol. 82, no. 1, 2007, pp. 50-3.
Saxena VK, Sundaresan NR, Malik F, et al. Temporal expression of transforming growth factor-beta2 and myostatin mRNA during embryonic myogenesis in Indian broilers. Res Vet Sci. 2007;82(1):50-3.
Saxena, V. K., Sundaresan, N. R., Malik, F., Ahmed, K. A., Saxena, M., Kumar, S., Nandedkar, P. V., & Singh, R. V. (2007). Temporal expression of transforming growth factor-beta2 and myostatin mRNA during embryonic myogenesis in Indian broilers. Research in Veterinary Science, 82(1), 50-3.
Saxena VK, et al. Temporal Expression of Transforming Growth Factor-beta2 and Myostatin mRNA During Embryonic Myogenesis in Indian Broilers. Res Vet Sci. 2007;82(1):50-3. PubMed PMID: 16870219.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal expression of transforming growth factor-beta2 and myostatin mRNA during embryonic myogenesis in Indian broilers. AU - Saxena,V K, AU - Sundaresan,N R, AU - Malik,Faizi, AU - Ahmed,K A, AU - Saxena,M, AU - Kumar,Sumit, AU - Nandedkar,P V, AU - Singh,R V, Y1 - 2006/07/25/ PY - 2005/07/11/received PY - 2006/05/10/revised PY - 2006/05/22/accepted PY - 2006/7/28/pubmed PY - 2007/3/3/medline PY - 2006/7/28/entrez SP - 50 EP - 3 JF - Research in veterinary science JO - Res Vet Sci VL - 82 IS - 1 N2 - TGF-beta2 and myostatin, the members of TGF family, act through both autocrine and paracrine mechanisms to regulate the growth and differentiation at various developmental stages in chicken. The kinetics and expression profile of these two growth factors were investigated by semi-quantitative RT-PCR, during the myogenesis of Indian broiler chickens. Total RNA was isolated from whole embryos on each of embryonic days (E) 0-6 (n=3 per day) and from the biceps femoris muscle at E7-E18 (n=3 per day). The expression of TGF-beta2 was noticed on E2 that remained at the same level until E6. In biceps femoris muscle, higher level of TGF-beta2 expression was observed during E7-E12, which decreased gradually thereafter. These findings suggested that TGF-beta2 might be a regulatory factor participating in the myogenesis of chicken embryos. Initial myostatin expression was noticed on E1, even before the myogenic lineage is established in embryo. This finding suggested an additional role of myostatin in early chicken embryo development, other than myogenesis. Furthermore, myostatin expression was significantly higher on E3 as compared to earlier studies, where initial higher level was observed at E2, suggesting the differential expression of myostatin among breeds. Higher and almost static myostatin expression was noticed in biceps femoris muscle during the entire period of myogenesis (E7-E18). In the present study, the ontogeny of myostatin expression coincided with myogenesis of chicken. Therefore, it may be hypothesized that myostatin is not only a major determinant of muscle mass, but also involved in early embryogenesis in chickens. SN - 0034-5288 UR - https://www.unboundmedicine.com/medline/citation/16870219/Temporal_expression_of_transforming_growth_factor_beta2_and_myostatin_mRNA_during_embryonic_myogenesis_in_Indian_broilers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0034-5288(06)00089-0 DB - PRIME DP - Unbound Medicine ER -