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Electrodeposition of polypyrrole-multiwalled carbon nanotube-glucose oxidase nanobiocomposite film for the detection of glucose.
Biosens Bioelectron. 2006 Oct 15; 22(4):495-500.BB

Abstract

A nanobiocomposite film consisted of polypyrrole (PPy), functionalized multiwalled carbon nanotubes (cMWNTs), and glucose oxidase (GOx) were electrochemically synthesized by electrooxidation of 0.1M pyrrole in aqueous solution containing appropriate amounts of cMWNTs and GOx. Potentiostatic growth profiles indicate that the anionic cMWNTs is incorporated within the growing PPy-cMWNTs nanocomposite for maintaining its electrical neutrality. The morphology of the PPy-cMWNTs nanocomposite was characterized by scanning electron microscopy (SEM). The PPy-cMWNTs nanocomposite was deposited homogeneously onto glassy carbon electrode. The amperometric responses vary proportionately to the concentration of hydrogen peroxide at the PPy-cMWNTs nanocomposite modified electrode at an operating potential of 0.7V versus Ag/AgCl (3M). The results indicate that the electroanalytical PPy-cMWNTs-GOx nanobiocomposite film was highly sensitive and suitable for glucose biosensor based on GOx function. The GOx concentration within the PPy-cMWNTs-GOx nanobiocomposite and the film thickness are crucial for the performance of the glucose biosensor. The amperometric responses of the optimized PPy-cMWNTs-GOx glucose biosensor (1.5 mgmL(-1) GOx, 141 mCcm(-2) total charge) displayed a sensitivity of 95 nAmM(-1), a linear range up to 4mM, and a response time of about 8s.

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Authors+Show Affiliations

Tsai YC
Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan. yctsai@dragonn.nchu.edu.tw
Li SC
No affiliation info available
Liao SW
No affiliation info available

MeSH

Biosensing TechniquesCoated Materials, BiocompatibleCrystallizationElectrochemistryElectroplatingEnzymes, ImmobilizedEquipment DesignEquipment Failure AnalysisGlucoseGlucose OxidaseMembranes, ArtificialMicroelectrodesNanotechnologyNanotubes, CarbonPolymersProtein BindingPyrrolesReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16870421

Citation

Tsai, Yu-Chen, et al. "Electrodeposition of Polypyrrole-multiwalled Carbon Nanotube-glucose Oxidase Nanobiocomposite Film for the Detection of Glucose." Biosensors & Bioelectronics, vol. 22, no. 4, 2006, pp. 495-500.
Tsai YC, Li SC, Liao SW. Electrodeposition of polypyrrole-multiwalled carbon nanotube-glucose oxidase nanobiocomposite film for the detection of glucose. Biosens Bioelectron. 2006;22(4):495-500.
Tsai, Y. C., Li, S. C., & Liao, S. W. (2006). Electrodeposition of polypyrrole-multiwalled carbon nanotube-glucose oxidase nanobiocomposite film for the detection of glucose. Biosensors & Bioelectronics, 22(4), 495-500.
Tsai YC, Li SC, Liao SW. Electrodeposition of Polypyrrole-multiwalled Carbon Nanotube-glucose Oxidase Nanobiocomposite Film for the Detection of Glucose. Biosens Bioelectron. 2006 Oct 15;22(4):495-500. PubMed PMID: 16870421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrodeposition of polypyrrole-multiwalled carbon nanotube-glucose oxidase nanobiocomposite film for the detection of glucose. AU - Tsai,Yu-Chen, AU - Li,Shih-Ci, AU - Liao,Shang-Wei, Y1 - 2006/07/25/ PY - 2006/02/14/received PY - 2006/05/16/revised PY - 2006/06/15/accepted PY - 2006/7/28/pubmed PY - 2007/1/18/medline PY - 2006/7/28/entrez SP - 495 EP - 500 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 4 N2 - A nanobiocomposite film consisted of polypyrrole (PPy), functionalized multiwalled carbon nanotubes (cMWNTs), and glucose oxidase (GOx) were electrochemically synthesized by electrooxidation of 0.1M pyrrole in aqueous solution containing appropriate amounts of cMWNTs and GOx. Potentiostatic growth profiles indicate that the anionic cMWNTs is incorporated within the growing PPy-cMWNTs nanocomposite for maintaining its electrical neutrality. The morphology of the PPy-cMWNTs nanocomposite was characterized by scanning electron microscopy (SEM). The PPy-cMWNTs nanocomposite was deposited homogeneously onto glassy carbon electrode. The amperometric responses vary proportionately to the concentration of hydrogen peroxide at the PPy-cMWNTs nanocomposite modified electrode at an operating potential of 0.7V versus Ag/AgCl (3M). The results indicate that the electroanalytical PPy-cMWNTs-GOx nanobiocomposite film was highly sensitive and suitable for glucose biosensor based on GOx function. The GOx concentration within the PPy-cMWNTs-GOx nanobiocomposite and the film thickness are crucial for the performance of the glucose biosensor. The amperometric responses of the optimized PPy-cMWNTs-GOx glucose biosensor (1.5 mgmL(-1) GOx, 141 mCcm(-2) total charge) displayed a sensitivity of 95 nAmM(-1), a linear range up to 4mM, and a response time of about 8s. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16870421/Electrodeposition_of_polypyrrole_multiwalled_carbon_nanotube_glucose_oxidase_nanobiocomposite_film_for_the_detection_of_glucose_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00285-5 DB - PRIME DP - Unbound Medicine ER -