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[Genetic and molecular study of inability of the yeast Kluyveromyces lactis var drosophilarum to ferment lactose].
Mikrobiologiia. 2006 May-Jun; 75(3):299-304.M

Abstract

The fermentation of lactose (Lac+) in the dairy yeast Kluyveromyces lactis var. lactis is controlled by the LAC4 (beta-galactosidase) and LAC12 (lactose permease) genes. The complementation analysis of twelve Kl. lactis var. drosophilarum natural homothallic Lac- strains of different origin was carried out using the genetic heterothallic lines of Kl. lactis var. lactis of the lac4LAC12 and LAC4lac12 genotypes. It was shown that the natural Lac- strains did not possess the LAC4LAC12 gene cluster. Southern hybridization of chromosomal DNA with LAC4 and LAC12 probes, as well as recombination analysis, showed that Kl. lactis var. drosophilarum yeasts do not have even silent copies of these genes. As distinct from this yeast, natural Lac- strains of the yeast Kl. marxianus are mutants impaired in the lactose permease gene (lac12 analogue), but possess an active beta-galactosidase gene (LAC4 analogue). The origin of the LAC4LAC12 gene cluster of the dairy yeasts Kl. lactis is discussed.

Authors

Naumov GI
No affiliation info available
Naumova ES
No affiliation info available
Barrio E
No affiliation info available
Querol A
No affiliation info available

MeSH

FermentationGenes, FungalGenetic Complementation TestKluyveromycesLactoseMembrane Transport ProteinsMultigene FamilyMutationbeta-Galactosidase

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

rus

PubMed ID

16871794

Citation

Naumov, G I., et al. "[Genetic and Molecular Study of Inability of the Yeast Kluyveromyces Lactis Var Drosophilarum to Ferment Lactose]." Mikrobiologiia, vol. 75, no. 3, 2006, pp. 299-304.
Naumov GI, Naumova ES, Barrio E, et al. [Genetic and molecular study of inability of the yeast Kluyveromyces lactis var drosophilarum to ferment lactose]. Mikrobiologiia. 2006;75(3):299-304.
Naumov, G. I., Naumova, E. S., Barrio, E., & Querol, A. (2006). [Genetic and molecular study of inability of the yeast Kluyveromyces lactis var drosophilarum to ferment lactose]. Mikrobiologiia, 75(3), 299-304.
Naumov GI, et al. [Genetic and Molecular Study of Inability of the Yeast Kluyveromyces Lactis Var Drosophilarum to Ferment Lactose]. Mikrobiologiia. 2006 May-Jun;75(3):299-304. PubMed PMID: 16871794.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Genetic and molecular study of inability of the yeast Kluyveromyces lactis var drosophilarum to ferment lactose]. AU - Naumov,G I, AU - Naumova,E S, AU - Barrio,E, AU - Querol,A, PY - 2006/7/29/pubmed PY - 2006/8/19/medline PY - 2006/7/29/entrez SP - 299 EP - 304 JF - Mikrobiologiia JO - Mikrobiologiia VL - 75 IS - 3 N2 - The fermentation of lactose (Lac+) in the dairy yeast Kluyveromyces lactis var. lactis is controlled by the LAC4 (beta-galactosidase) and LAC12 (lactose permease) genes. The complementation analysis of twelve Kl. lactis var. drosophilarum natural homothallic Lac- strains of different origin was carried out using the genetic heterothallic lines of Kl. lactis var. lactis of the lac4LAC12 and LAC4lac12 genotypes. It was shown that the natural Lac- strains did not possess the LAC4LAC12 gene cluster. Southern hybridization of chromosomal DNA with LAC4 and LAC12 probes, as well as recombination analysis, showed that Kl. lactis var. drosophilarum yeasts do not have even silent copies of these genes. As distinct from this yeast, natural Lac- strains of the yeast Kl. marxianus are mutants impaired in the lactose permease gene (lac12 analogue), but possess an active beta-galactosidase gene (LAC4 analogue). The origin of the LAC4LAC12 gene cluster of the dairy yeasts Kl. lactis is discussed. SN - 0026-3656 UR - https://www.unboundmedicine.com/medline/citation/16871794/[Genetic_and_molecular_study_of_inability_of_the_yeast_Kluyveromyces_lactis_var_drosophilarum_to_ferment_lactose]_ DB - PRIME DP - Unbound Medicine ER -