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Experimental manipulations of microbial food web interactions in a humic lake: shifting biological drivers of bacterial community structure.
Environ Microbiol. 2006 Aug; 8(8):1448-59.EM

Abstract

A previous multiyear study observed correlations between bacterioplankton community composition (BCC) and abundance and the dynamics of phytoplankton populations and bacterivorous grazers in a humic lake. These observations generated hypotheses about the importance of trophic interactions (both top-down and bottom-up) for structuring bacterial communities in this lake, which were tested using two multifactorial food web manipulation experiments that separately manipulated the intensity of grazing and the composition of the phytoplankton community. Our results, combined with field observations, suggest that a hierarchy of drivers structures bacterial communities in this lake. While other studies have noted links between aggregate measures of phytoplankton and bacterioplankton communities, we demonstrate here correlations between succession of phytoplankton assemblages and BCC as assessed by automated ribosomal intergenic spacer analysis (ARISA). We used a novel approach linking community ARISA data to phylogenetic assignments from sequence analysis of 16S rRNA gene clone libraries to examine the responses of specific bacterial phylotypes to the experimental manipulations. The synchronous dynamics of these populations suggests that primary producers may mediate BCC and diversity through labile organic matter production, which evolves in quality and quantity during phytoplankton succession. Superimposed on this resource-mediated control of BCC are brief periods of intense bacterivory that impact bacterial abundance and composition.

Authors+Show Affiliations

Center for Limnology, University of Wisconsin-Madison, Madison, WI 53706, USA. akent@uiuc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16872407

Citation

Kent, Angela D., et al. "Experimental Manipulations of Microbial Food Web Interactions in a Humic Lake: Shifting Biological Drivers of Bacterial Community Structure." Environmental Microbiology, vol. 8, no. 8, 2006, pp. 1448-59.
Kent AD, Jones SE, Lauster GH, et al. Experimental manipulations of microbial food web interactions in a humic lake: shifting biological drivers of bacterial community structure. Environ Microbiol. 2006;8(8):1448-59.
Kent, A. D., Jones, S. E., Lauster, G. H., Graham, J. M., Newton, R. J., & McMahon, K. D. (2006). Experimental manipulations of microbial food web interactions in a humic lake: shifting biological drivers of bacterial community structure. Environmental Microbiology, 8(8), 1448-59.
Kent AD, et al. Experimental Manipulations of Microbial Food Web Interactions in a Humic Lake: Shifting Biological Drivers of Bacterial Community Structure. Environ Microbiol. 2006;8(8):1448-59. PubMed PMID: 16872407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental manipulations of microbial food web interactions in a humic lake: shifting biological drivers of bacterial community structure. AU - Kent,Angela D, AU - Jones,Stuart E, AU - Lauster,George H, AU - Graham,James M, AU - Newton,Ryan J, AU - McMahon,Katherine D, PY - 2006/7/29/pubmed PY - 2006/10/4/medline PY - 2006/7/29/entrez SP - 1448 EP - 59 JF - Environmental microbiology JO - Environ Microbiol VL - 8 IS - 8 N2 - A previous multiyear study observed correlations between bacterioplankton community composition (BCC) and abundance and the dynamics of phytoplankton populations and bacterivorous grazers in a humic lake. These observations generated hypotheses about the importance of trophic interactions (both top-down and bottom-up) for structuring bacterial communities in this lake, which were tested using two multifactorial food web manipulation experiments that separately manipulated the intensity of grazing and the composition of the phytoplankton community. Our results, combined with field observations, suggest that a hierarchy of drivers structures bacterial communities in this lake. While other studies have noted links between aggregate measures of phytoplankton and bacterioplankton communities, we demonstrate here correlations between succession of phytoplankton assemblages and BCC as assessed by automated ribosomal intergenic spacer analysis (ARISA). We used a novel approach linking community ARISA data to phylogenetic assignments from sequence analysis of 16S rRNA gene clone libraries to examine the responses of specific bacterial phylotypes to the experimental manipulations. The synchronous dynamics of these populations suggests that primary producers may mediate BCC and diversity through labile organic matter production, which evolves in quality and quantity during phytoplankton succession. Superimposed on this resource-mediated control of BCC are brief periods of intense bacterivory that impact bacterial abundance and composition. SN - 1462-2912 UR - https://www.unboundmedicine.com/medline/citation/16872407/Experimental_manipulations_of_microbial_food_web_interactions_in_a_humic_lake:_shifting_biological_drivers_of_bacterial_community_structure_ L2 - https://doi.org/10.1111/j.1462-2920.2006.01039.x DB - PRIME DP - Unbound Medicine ER -