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GC-MS-MS determination of gamma-hydroxybutyrate in blood and urine.
J Anal Toxicol. 2006 Jul-Aug; 30(6):375-9.JA

Abstract

A gas chromatographic-tandem mass spectrometric (GC-MS-MS) method for determining trace concentrations of gamma-hydroxybutyrate (GHB) in blood and urine has been developed. Multiple reaction monitoring was used to detect parent and daughter ions of GHB, 233 and 147, respectively, following liquid-liquid extraction with acetonitrile and derivatisation with N,O-bis[trimethylsilyl]trifluoroacetamide (BSTFA). Deuterated GHB was used as an internal standard. The assay produced excellent linearity and sensitivity without conversion to gamma butyrolactone. The lower limit of quantitation (LLOQ) in 50 microL of sample was 2.5 microg/mL. The expanded uncertainty values for intra- and interassay results were +/- 0.097 and +/- 0.123 ng/mL at a confidence level of 95% for blood and urine, respectively. Endogenous concentrations of GHB were found to be in the range of 0.3 to 6 microg/mL in urine and 0.5 to 2.3 microg/mL in blood, confirming previously suggested cut-off values for forensic analysis.

Authors+Show Affiliations

University of Glamorgan, Pontypridd, CF37 1DL, Wales. richpaul@talk21.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16872568

Citation

Paul, Richard, et al. "GC-MS-MS Determination of Gamma-hydroxybutyrate in Blood and Urine." Journal of Analytical Toxicology, vol. 30, no. 6, 2006, pp. 375-9.
Paul R, Tsanaclis L, Kingston R, et al. GC-MS-MS determination of gamma-hydroxybutyrate in blood and urine. J Anal Toxicol. 2006;30(6):375-9.
Paul, R., Tsanaclis, L., Kingston, R., Berry, A., & Guwy, A. (2006). GC-MS-MS determination of gamma-hydroxybutyrate in blood and urine. Journal of Analytical Toxicology, 30(6), 375-9.
Paul R, et al. GC-MS-MS Determination of Gamma-hydroxybutyrate in Blood and Urine. J Anal Toxicol. 2006 Jul-Aug;30(6):375-9. PubMed PMID: 16872568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - GC-MS-MS determination of gamma-hydroxybutyrate in blood and urine. AU - Paul,Richard, AU - Tsanaclis,Lolita, AU - Kingston,Robert, AU - Berry,Anthony, AU - Guwy,Alan, PY - 2006/7/29/pubmed PY - 2007/8/9/medline PY - 2006/7/29/entrez SP - 375 EP - 9 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 30 IS - 6 N2 - A gas chromatographic-tandem mass spectrometric (GC-MS-MS) method for determining trace concentrations of gamma-hydroxybutyrate (GHB) in blood and urine has been developed. Multiple reaction monitoring was used to detect parent and daughter ions of GHB, 233 and 147, respectively, following liquid-liquid extraction with acetonitrile and derivatisation with N,O-bis[trimethylsilyl]trifluoroacetamide (BSTFA). Deuterated GHB was used as an internal standard. The assay produced excellent linearity and sensitivity without conversion to gamma butyrolactone. The lower limit of quantitation (LLOQ) in 50 microL of sample was 2.5 microg/mL. The expanded uncertainty values for intra- and interassay results were +/- 0.097 and +/- 0.123 ng/mL at a confidence level of 95% for blood and urine, respectively. Endogenous concentrations of GHB were found to be in the range of 0.3 to 6 microg/mL in urine and 0.5 to 2.3 microg/mL in blood, confirming previously suggested cut-off values for forensic analysis. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/16872568/GC_MS_MS_determination_of_gamma_hydroxybutyrate_in_blood_and_urine_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/30.6.375 DB - PRIME DP - Unbound Medicine ER -