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Differential expression of placental and vascular endothelial nitric oxide synthase in an ovine model of fetal growth restriction.
Am J Obstet Gynecol. 2006 Sep; 195(3):771-7.AJ

Abstract

OBJECTIVE

The objective of the study was to evaluate endothelial nitric oxide synthase concentration in the placenta, uterine, and umbilical vessels near term in an ovine model of intrauterine growth restriction induced by hyperthermia beginning in early gestation.

STUDY DESIGN

Four pregnant ewes were exposed to hyperthermia conditions for 80 days beginning at 35 days gestation to induce intrauterine growth restriction. Four ewes were kept in ambient conditions as controls. Umbilical artery Doppler systolic to diastolic ratios were calculated. At 128 days gestation, fetal catheters were placed for aortic blood pressure measurements and blood gas determination. At 132 days gestation, fetal mean systemic blood pressure and gases were determined. Sheep placentomes, umbilical artery and vein, and uterine artery were assessed for endothelial nitric oxide synthase concentration and immunolocalization.

RESULTS

Compared with control pregnancies, the intrauterine growth restriction pregnancies showed: (1) reduced fetal and placental weights (P < or = .01); (2) elevated systemic blood pressure (41 +/- 1.53 mm Hg versus 44.3 +/- 1.71 mm Hg; P < or = .05) and systolic to diastolic ratios (3.0 +/- 0.34 versus 3.8 +/- 0.18; P < or = .01); (3) reduced fetal O2 saturation (52.2 +/- 7.03% versus 33.05 +/- 10.98%; P < or = .008); and (4) decreased endothelial nitric oxide synthase protein concentration in the umbilical artery (2.7-fold; P < or = .01) and a trend for a decrease in the uterine artery (1.4-fold; P < or = .1).

CONCLUSION

We conclude that placental endothelial nitric oxide synthase protein concentration is increased near term in our ovine model of intrauterine growth restriction, and that this increase may be secondary to hypoxia.

Authors+Show Affiliations

Division of Perinatal Medicine, Department of Obstetrics and Gynecology, University of Colorado Health Sciences Center, Denver, CO 80262, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16875646

Citation

Arroyo, Juan A., et al. "Differential Expression of Placental and Vascular Endothelial Nitric Oxide Synthase in an Ovine Model of Fetal Growth Restriction." American Journal of Obstetrics and Gynecology, vol. 195, no. 3, 2006, pp. 771-7.
Arroyo JA, Anthony RV, Parker TA, et al. Differential expression of placental and vascular endothelial nitric oxide synthase in an ovine model of fetal growth restriction. Am J Obstet Gynecol. 2006;195(3):771-7.
Arroyo, J. A., Anthony, R. V., Parker, T. A., & Galan, H. L. (2006). Differential expression of placental and vascular endothelial nitric oxide synthase in an ovine model of fetal growth restriction. American Journal of Obstetrics and Gynecology, 195(3), 771-7.
Arroyo JA, et al. Differential Expression of Placental and Vascular Endothelial Nitric Oxide Synthase in an Ovine Model of Fetal Growth Restriction. Am J Obstet Gynecol. 2006;195(3):771-7. PubMed PMID: 16875646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of placental and vascular endothelial nitric oxide synthase in an ovine model of fetal growth restriction. AU - Arroyo,Juan A, AU - Anthony,Russell V, AU - Parker,Thomas A, AU - Galan,Henry L, Y1 - 2006/07/26/ PY - 2006/03/14/received PY - 2006/05/02/revised PY - 2006/06/02/accepted PY - 2006/8/1/pubmed PY - 2006/10/7/medline PY - 2006/8/1/entrez SP - 771 EP - 7 JF - American journal of obstetrics and gynecology JO - Am J Obstet Gynecol VL - 195 IS - 3 N2 - OBJECTIVE: The objective of the study was to evaluate endothelial nitric oxide synthase concentration in the placenta, uterine, and umbilical vessels near term in an ovine model of intrauterine growth restriction induced by hyperthermia beginning in early gestation. STUDY DESIGN: Four pregnant ewes were exposed to hyperthermia conditions for 80 days beginning at 35 days gestation to induce intrauterine growth restriction. Four ewes were kept in ambient conditions as controls. Umbilical artery Doppler systolic to diastolic ratios were calculated. At 128 days gestation, fetal catheters were placed for aortic blood pressure measurements and blood gas determination. At 132 days gestation, fetal mean systemic blood pressure and gases were determined. Sheep placentomes, umbilical artery and vein, and uterine artery were assessed for endothelial nitric oxide synthase concentration and immunolocalization. RESULTS: Compared with control pregnancies, the intrauterine growth restriction pregnancies showed: (1) reduced fetal and placental weights (P < or = .01); (2) elevated systemic blood pressure (41 +/- 1.53 mm Hg versus 44.3 +/- 1.71 mm Hg; P < or = .05) and systolic to diastolic ratios (3.0 +/- 0.34 versus 3.8 +/- 0.18; P < or = .01); (3) reduced fetal O2 saturation (52.2 +/- 7.03% versus 33.05 +/- 10.98%; P < or = .008); and (4) decreased endothelial nitric oxide synthase protein concentration in the umbilical artery (2.7-fold; P < or = .01) and a trend for a decrease in the uterine artery (1.4-fold; P < or = .1). CONCLUSION: We conclude that placental endothelial nitric oxide synthase protein concentration is increased near term in our ovine model of intrauterine growth restriction, and that this increase may be secondary to hypoxia. SN - 1097-6868 UR - https://www.unboundmedicine.com/medline/citation/16875646/Differential_expression_of_placental_and_vascular_endothelial_nitric_oxide_synthase_in_an_ovine_model_of_fetal_growth_restriction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9378(06)00742-3 DB - PRIME DP - Unbound Medicine ER -