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Neuroprotection of alpha-phenyl-n-tert-butyl-nitrone on the neonatal white matter is associated with anti-inflammation.
Neurosci Lett. 2006 Sep 11; 405(1-2):52-6.NL

Abstract

Our previous study has demonstrated that alpha-phenyl-tert-butyl-nitrone (PBN) provided neuroprotection to the neonatal white matter following cerebral hypoxia-ischemia (HI). Free radical scavenging was involved in the neuroprotection of PBN. To investigate if other mechanisms contribute to the neuroprotection of PBN, postnatal day 4 SD rats were subjected to bilateral common carotid artery ligation, followed by 8% oxygen exposure for 20min. A single dose of PBN (100mg/kg, i.p.) was given prior to the hypoxic exposure. Expression of inflammatory cytokines: interleukin-1beta (IL-1beta), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-alpha) was determined by RT-PCR, ELISA and immunohistochemistry. Activation of transcriptional factor nuclear factor-kappa B (NF-kappaB) was measured by ELISA. PBN significantly inhibited HI-induced up-regulation of IL-1beta, TNF-alpha and iNOS mRNA expression at 4h following HI. PBN treatment also reduced the brain concentration of IL-1beta significantly and decreased the number of IL-1beta- or iNOS-expressing cells in the white matter area at 12h following HI. Moreover, PBN suppressed the HI-induced NF-kappaB activation at 1h after HI. The overall results indicate that besides free radical scavenging, anti-inflammation might partly contribute to the neuroprotection afforded by PBN on neonatal white matter following cerebral HI.

Authors+Show Affiliations

Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, United States.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16876321

Citation

Lin, Shuying, et al. "Neuroprotection of Alpha-phenyl-n-tert-butyl-nitrone On the Neonatal White Matter Is Associated With Anti-inflammation." Neuroscience Letters, vol. 405, no. 1-2, 2006, pp. 52-6.
Lin S, Cox HJ, Rhodes PG, et al. Neuroprotection of alpha-phenyl-n-tert-butyl-nitrone on the neonatal white matter is associated with anti-inflammation. Neurosci Lett. 2006;405(1-2):52-6.
Lin, S., Cox, H. J., Rhodes, P. G., & Cai, Z. (2006). Neuroprotection of alpha-phenyl-n-tert-butyl-nitrone on the neonatal white matter is associated with anti-inflammation. Neuroscience Letters, 405(1-2), 52-6.
Lin S, et al. Neuroprotection of Alpha-phenyl-n-tert-butyl-nitrone On the Neonatal White Matter Is Associated With Anti-inflammation. Neurosci Lett. 2006 Sep 11;405(1-2):52-6. PubMed PMID: 16876321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotection of alpha-phenyl-n-tert-butyl-nitrone on the neonatal white matter is associated with anti-inflammation. AU - Lin,Shuying, AU - Cox,Helen J, AU - Rhodes,Philip G, AU - Cai,Zhengwei, Y1 - 2006/07/28/ PY - 2006/05/16/received PY - 2006/06/13/revised PY - 2006/06/15/accepted PY - 2006/8/1/pubmed PY - 2006/10/25/medline PY - 2006/8/1/entrez SP - 52 EP - 6 JF - Neuroscience letters JO - Neurosci Lett VL - 405 IS - 1-2 N2 - Our previous study has demonstrated that alpha-phenyl-tert-butyl-nitrone (PBN) provided neuroprotection to the neonatal white matter following cerebral hypoxia-ischemia (HI). Free radical scavenging was involved in the neuroprotection of PBN. To investigate if other mechanisms contribute to the neuroprotection of PBN, postnatal day 4 SD rats were subjected to bilateral common carotid artery ligation, followed by 8% oxygen exposure for 20min. A single dose of PBN (100mg/kg, i.p.) was given prior to the hypoxic exposure. Expression of inflammatory cytokines: interleukin-1beta (IL-1beta), inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-alpha) was determined by RT-PCR, ELISA and immunohistochemistry. Activation of transcriptional factor nuclear factor-kappa B (NF-kappaB) was measured by ELISA. PBN significantly inhibited HI-induced up-regulation of IL-1beta, TNF-alpha and iNOS mRNA expression at 4h following HI. PBN treatment also reduced the brain concentration of IL-1beta significantly and decreased the number of IL-1beta- or iNOS-expressing cells in the white matter area at 12h following HI. Moreover, PBN suppressed the HI-induced NF-kappaB activation at 1h after HI. The overall results indicate that besides free radical scavenging, anti-inflammation might partly contribute to the neuroprotection afforded by PBN on neonatal white matter following cerebral HI. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/16876321/Neuroprotection_of_alpha_phenyl_n_tert_butyl_nitrone_on_the_neonatal_white_matter_is_associated_with_anti_inflammation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(06)00622-7 DB - PRIME DP - Unbound Medicine ER -