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The effects of paeonol on the electrophysiological properties of cardiac ventricular myocytes.
Eur J Pharmacol. 2006 Sep 18; 545(2-3):87-92.EJ

Abstract

Previous studies have shown that "Mudanpi", a Chinese herbal medicine, has a significant cardioprotective effect against myocardial ischaemia. Based on these findings we hypothesised that paeonol, the main component of Mudanpi, might have an effect on the cellular electrophysiology of cardiac ventricular myocytes. The effects of paeonol on the action potential and ion channels of cardiac ventricular myocytes were studied using the standard whole-cell configuration of the patch-clamp technique. Ventricular myocytes were isolated from the hearts of adult guinea-pig by enzymic dispersion. The myocytes were continuously perfused with various experimental solutions at room temperature and paeonol applied in the perfusate. Action potentials and membrane currents were recorded using both current and voltage clamp modes of the patch-clamp technique. Paeonol, at concentrations 160 microM and 640 microM, decreased the action potential upstroke phase, an action associated with the blockade of the voltage-gated, fast sodium channel. The effects of paeonol on both action potential and Na(+) current were concentration dependent. Paeonol had a high affinity for inactivated sodium channels. Paeonol also shortened the action potential duration, in a manner not associated with the blockade of the calcium current, or the enhancement of potassium currents. These findings suggest that paeonol, and therefore Mudanpi, may possess antiarrhythmic activity, which may confer its cardioprotective effects.

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Authors+Show Affiliations

Ma YL
Department of Pharmacology, United Medical and Dental Schools, St. Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, UK. y.ma@reading.ac.uk
Bates S
No affiliation info available
Gurney AM
No affiliation info available

MeSH

AcetophenonesAction PotentialsAnimalsGuinea PigsHeart VentriclesMaleMyocytes, CardiacSodium Channel BlockersSodium Channels

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16876781

Citation

Ma, Yu-ling, et al. "The Effects of Paeonol On the Electrophysiological Properties of Cardiac Ventricular Myocytes." European Journal of Pharmacology, vol. 545, no. 2-3, 2006, pp. 87-92.
Ma YL, Bates S, Gurney AM. The effects of paeonol on the electrophysiological properties of cardiac ventricular myocytes. Eur J Pharmacol. 2006;545(2-3):87-92.
Ma, Y. L., Bates, S., & Gurney, A. M. (2006). The effects of paeonol on the electrophysiological properties of cardiac ventricular myocytes. European Journal of Pharmacology, 545(2-3), 87-92.
Ma YL, Bates S, Gurney AM. The Effects of Paeonol On the Electrophysiological Properties of Cardiac Ventricular Myocytes. Eur J Pharmacol. 2006 Sep 18;545(2-3):87-92. PubMed PMID: 16876781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of paeonol on the electrophysiological properties of cardiac ventricular myocytes. AU - Ma,Yu-ling, AU - Bates,Susan, AU - Gurney,Alison M, Y1 - 2006/07/01/ PY - 2005/11/03/received PY - 2006/05/23/revised PY - 2006/06/20/accepted PY - 2006/8/1/pubmed PY - 2006/11/14/medline PY - 2006/8/1/entrez SP - 87 EP - 92 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 545 IS - 2-3 N2 - Previous studies have shown that "Mudanpi", a Chinese herbal medicine, has a significant cardioprotective effect against myocardial ischaemia. Based on these findings we hypothesised that paeonol, the main component of Mudanpi, might have an effect on the cellular electrophysiology of cardiac ventricular myocytes. The effects of paeonol on the action potential and ion channels of cardiac ventricular myocytes were studied using the standard whole-cell configuration of the patch-clamp technique. Ventricular myocytes were isolated from the hearts of adult guinea-pig by enzymic dispersion. The myocytes were continuously perfused with various experimental solutions at room temperature and paeonol applied in the perfusate. Action potentials and membrane currents were recorded using both current and voltage clamp modes of the patch-clamp technique. Paeonol, at concentrations 160 microM and 640 microM, decreased the action potential upstroke phase, an action associated with the blockade of the voltage-gated, fast sodium channel. The effects of paeonol on both action potential and Na(+) current were concentration dependent. Paeonol had a high affinity for inactivated sodium channels. Paeonol also shortened the action potential duration, in a manner not associated with the blockade of the calcium current, or the enhancement of potassium currents. These findings suggest that paeonol, and therefore Mudanpi, may possess antiarrhythmic activity, which may confer its cardioprotective effects. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/16876781/The_effects_of_paeonol_on_the_electrophysiological_properties_of_cardiac_ventricular_myocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(06)00675-3 DB - PRIME DP - Unbound Medicine ER -