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Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos.
Exp Cell Res. 2006 Oct 15; 312(17):3260-75.EC

Abstract

Cytokinesis is the final stage in cell division that serves to partition cytoplasm and daughter nuclei into separate cells. Membrane remodeling at the cleavage plane is a required feature of cytokinesis in many species. In animal cells, however, the precise mechanisms and molecular interactions that mediate this process are not yet fully understood. Using real-time imaging in live, early stage zebrafish embryos, we demonstrate that vesicles labeled with the v-SNARE, VAMP-2, are recruited to the cleavage furrow during deepening in a microtubule-dependent manner. These vesicles then fuse with, and transfer their VAMP-2 fluorescent label to, the plasma membrane during both furrow deepening and subsequent apposition. This observation indicates that new membrane is being inserted during these stages of cytokinesis. Inhibition of SNAP-25 (a cognate t-SNARE of VAMP-2), using a monoclonal antibody, blocked VAMP-2 vesicle fusion and furrow apposition. Transient expression of mutant forms of SNAP-25 also produced defects in furrow apposition. SNAP-25 inhibition by either method, however, did not have any significant effect on furrow deepening. Thus, our data clearly indicate that VAMP-2 and SNAP-25 play an essential role in daughter blastomere apposition, possibly via the delivery of components that promote the cell-to-cell adhesion required for the successful completion of cytokinesis. Our results also support the idea that new membrane addition, which occurs during late stage cytokinesis, is not required for furrow deepening that results from contractile band constriction.

Authors+Show Affiliations

Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, PRC.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16876784

Citation

Li, Wai Ming, et al. "Recruitment and SNARE-mediated Fusion of Vesicles in Furrow Membrane Remodeling During Cytokinesis in Zebrafish Embryos." Experimental Cell Research, vol. 312, no. 17, 2006, pp. 3260-75.
Li WM, Webb SE, Lee KW, et al. Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos. Exp Cell Res. 2006;312(17):3260-75.
Li, W. M., Webb, S. E., Lee, K. W., & Miller, A. L. (2006). Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos. Experimental Cell Research, 312(17), 3260-75.
Li WM, et al. Recruitment and SNARE-mediated Fusion of Vesicles in Furrow Membrane Remodeling During Cytokinesis in Zebrafish Embryos. Exp Cell Res. 2006 Oct 15;312(17):3260-75. PubMed PMID: 16876784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos. AU - Li,Wai Ming, AU - Webb,Sarah E, AU - Lee,Karen W, AU - Miller,Andrew L, Y1 - 2006/06/27/ PY - 2006/04/11/received PY - 2006/06/14/revised PY - 2006/06/19/accepted PY - 2006/8/1/pubmed PY - 2006/11/7/medline PY - 2006/8/1/entrez SP - 3260 EP - 75 JF - Experimental cell research JO - Exp. Cell Res. VL - 312 IS - 17 N2 - Cytokinesis is the final stage in cell division that serves to partition cytoplasm and daughter nuclei into separate cells. Membrane remodeling at the cleavage plane is a required feature of cytokinesis in many species. In animal cells, however, the precise mechanisms and molecular interactions that mediate this process are not yet fully understood. Using real-time imaging in live, early stage zebrafish embryos, we demonstrate that vesicles labeled with the v-SNARE, VAMP-2, are recruited to the cleavage furrow during deepening in a microtubule-dependent manner. These vesicles then fuse with, and transfer their VAMP-2 fluorescent label to, the plasma membrane during both furrow deepening and subsequent apposition. This observation indicates that new membrane is being inserted during these stages of cytokinesis. Inhibition of SNAP-25 (a cognate t-SNARE of VAMP-2), using a monoclonal antibody, blocked VAMP-2 vesicle fusion and furrow apposition. Transient expression of mutant forms of SNAP-25 also produced defects in furrow apposition. SNAP-25 inhibition by either method, however, did not have any significant effect on furrow deepening. Thus, our data clearly indicate that VAMP-2 and SNAP-25 play an essential role in daughter blastomere apposition, possibly via the delivery of components that promote the cell-to-cell adhesion required for the successful completion of cytokinesis. Our results also support the idea that new membrane addition, which occurs during late stage cytokinesis, is not required for furrow deepening that results from contractile band constriction. SN - 0014-4827 UR - https://www.unboundmedicine.com/medline/citation/16876784/Recruitment_and_SNARE_mediated_fusion_of_vesicles_in_furrow_membrane_remodeling_during_cytokinesis_in_zebrafish_embryos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(06)00252-7 DB - PRIME DP - Unbound Medicine ER -