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Myosin heavy chain isoform transitions in canine skeletal muscles during postnatal growth.
J Anat. 2006 Aug; 209(2):149-63.JA

Abstract

To gain a better understanding of the normal characteristics of developing canine muscles, myosin heavy chain (MHC) isoform expression was analysed in the axial and limb skeletal muscles of 18 young dogs whose ages ranged from the late prenatal stage to 6 months. We compared the results of immunohistochemistry using ten monoclonal antibodies, specific to different MHC isoforms, and enzyme-histochemical reactions, which demonstrate the activity of myofibrillar ATPase, succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (alpha-GPDH). In the skeletal muscles of fetuses and neonatal dogs the developmental isoforms MHC-emb and MHC-neo were prevalent. In all muscles the primary fibres, located centrally in each muscle fascicle, strongly expressed the slow isoform MHC-I. The adult fast isoform MHC-IIa was first noted in some of the secondary fibres on fetal day 55. During the first 10 days after birth, the expression of MHC-emb declined, as did that of MHC-neo during the second and third weeks. Correspondingly, the expression of MHC-IIa, and later, of MHC-I increased in the secondary fibres. Between the sixth week and second month the expression of MHC-IIx became prominent. The slow rhomboideus muscle exhibited an early expression of the slow isoform in the secondary fibres. Our results indicate that the timing of muscle maturation depends on its activity immediately following birth. The fastest developing muscle was the diaphragm, followed by the fast muscles. A pronounced changeover from developmental to adult isoforms was noted at 4-6 weeks of age, which coincides with the increased physical activity of puppies.

Authors+Show Affiliations

Institute of Anatomy, Histology and Embryology, Veterinary Faculty, University of Ljubljana, Slovenia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16879596

Citation

Strbenc, Malan, et al. "Myosin Heavy Chain Isoform Transitions in Canine Skeletal Muscles During Postnatal Growth." Journal of Anatomy, vol. 209, no. 2, 2006, pp. 149-63.
Strbenc M, Smerdu V, Pogacnik A, et al. Myosin heavy chain isoform transitions in canine skeletal muscles during postnatal growth. J Anat. 2006;209(2):149-63.
Strbenc, M., Smerdu, V., Pogacnik, A., & Fazarinc, G. (2006). Myosin heavy chain isoform transitions in canine skeletal muscles during postnatal growth. Journal of Anatomy, 209(2), 149-63.
Strbenc M, et al. Myosin Heavy Chain Isoform Transitions in Canine Skeletal Muscles During Postnatal Growth. J Anat. 2006;209(2):149-63. PubMed PMID: 16879596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Myosin heavy chain isoform transitions in canine skeletal muscles during postnatal growth. AU - Strbenc,Malan, AU - Smerdu,Vika, AU - Pogacnik,Azra, AU - Fazarinc,Gregor, PY - 2006/8/2/pubmed PY - 2007/4/4/medline PY - 2006/8/2/entrez SP - 149 EP - 63 JF - Journal of anatomy JO - J Anat VL - 209 IS - 2 N2 - To gain a better understanding of the normal characteristics of developing canine muscles, myosin heavy chain (MHC) isoform expression was analysed in the axial and limb skeletal muscles of 18 young dogs whose ages ranged from the late prenatal stage to 6 months. We compared the results of immunohistochemistry using ten monoclonal antibodies, specific to different MHC isoforms, and enzyme-histochemical reactions, which demonstrate the activity of myofibrillar ATPase, succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (alpha-GPDH). In the skeletal muscles of fetuses and neonatal dogs the developmental isoforms MHC-emb and MHC-neo were prevalent. In all muscles the primary fibres, located centrally in each muscle fascicle, strongly expressed the slow isoform MHC-I. The adult fast isoform MHC-IIa was first noted in some of the secondary fibres on fetal day 55. During the first 10 days after birth, the expression of MHC-emb declined, as did that of MHC-neo during the second and third weeks. Correspondingly, the expression of MHC-IIa, and later, of MHC-I increased in the secondary fibres. Between the sixth week and second month the expression of MHC-IIx became prominent. The slow rhomboideus muscle exhibited an early expression of the slow isoform in the secondary fibres. Our results indicate that the timing of muscle maturation depends on its activity immediately following birth. The fastest developing muscle was the diaphragm, followed by the fast muscles. A pronounced changeover from developmental to adult isoforms was noted at 4-6 weeks of age, which coincides with the increased physical activity of puppies. SN - 0021-8782 UR - https://www.unboundmedicine.com/medline/citation/16879596/Myosin_heavy_chain_isoform_transitions_in_canine_skeletal_muscles_during_postnatal_growth_ L2 - https://doi.org/10.1111/j.1469-7580.2006.00599.x DB - PRIME DP - Unbound Medicine ER -