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Gross, histologic, and gene expression characteristics of osteoarthritic articular cartilage of the metacarpal condyle of horses.
Am J Vet Res. 2006 Aug; 67(8):1299-306.AJ

Abstract

OBJECTIVE

To identify patterns and correlations of gross, histologic, and gene expression characteristics of articular cartilage from horses with osteoarthritis.

ANIMALS

10 clinically normal horses and 11 horses with osteoarthritis of the metacarpal condyles.

PROCEDURES

Metacarpophalangeal joints were opened and digitally photographed, and gross lesions were scored and quantified. Representative cartilage specimens were stained for histologic scoring. Total RNA from dorsal and palmar articular surfaces was processed on an equine gene expression microarray.

RESULTS

Histologic scores were greater in both regions of osteoarthritic joints, compared with corresponding regions in control joints. Cartilage from the palmar aspect of diseased joints had the highest histologic scores of osteoarthritic sites or of either region in control joints. A different set of genes for dorsal and palmar osteoarthritis was identified for high and low gene expression. Articular cartilage from the dorsal region had surface fraying and greater expression of genes coding for collagen matrix components and proteins with anti-apoptotic function, compared with control specimens. Articular cartilage from the palmar region had greater fraying, deep fissures, and less expression of genes coding for glycosaminoglycan matrix formation and proteins with anti-apoptotic function, compared with cartilage from disease-free joints and the dorsal aspect of affected joints.

CONCLUSIONS AND CLINICAL RELEVANCE

Metacarpal condyles of horses with naturally occurring osteoarthritis had an identifiable and regional gene expression signature with typical morphologic features.

Authors+Show Affiliations

Comparative Orthopedic Research Laboratories, Department of Veterinary Clinical Sciences, The Ohio State University, Columbus, 43210, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16881840

Citation

Smith, Katie J., et al. "Gross, Histologic, and Gene Expression Characteristics of Osteoarthritic Articular Cartilage of the Metacarpal Condyle of Horses." American Journal of Veterinary Research, vol. 67, no. 8, 2006, pp. 1299-306.
Smith KJ, Bertone AL, Weisbrode SE, et al. Gross, histologic, and gene expression characteristics of osteoarthritic articular cartilage of the metacarpal condyle of horses. Am J Vet Res. 2006;67(8):1299-306.
Smith, K. J., Bertone, A. L., Weisbrode, S. E., & Radmacher, M. (2006). Gross, histologic, and gene expression characteristics of osteoarthritic articular cartilage of the metacarpal condyle of horses. American Journal of Veterinary Research, 67(8), 1299-306.
Smith KJ, et al. Gross, Histologic, and Gene Expression Characteristics of Osteoarthritic Articular Cartilage of the Metacarpal Condyle of Horses. Am J Vet Res. 2006;67(8):1299-306. PubMed PMID: 16881840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gross, histologic, and gene expression characteristics of osteoarthritic articular cartilage of the metacarpal condyle of horses. AU - Smith,Katie J, AU - Bertone,Alicia L, AU - Weisbrode,Stephen E, AU - Radmacher,Michael, PY - 2006/8/3/pubmed PY - 2006/10/27/medline PY - 2006/8/3/entrez SP - 1299 EP - 306 JF - American journal of veterinary research JO - Am. J. Vet. Res. VL - 67 IS - 8 N2 - OBJECTIVE: To identify patterns and correlations of gross, histologic, and gene expression characteristics of articular cartilage from horses with osteoarthritis. ANIMALS: 10 clinically normal horses and 11 horses with osteoarthritis of the metacarpal condyles. PROCEDURES: Metacarpophalangeal joints were opened and digitally photographed, and gross lesions were scored and quantified. Representative cartilage specimens were stained for histologic scoring. Total RNA from dorsal and palmar articular surfaces was processed on an equine gene expression microarray. RESULTS: Histologic scores were greater in both regions of osteoarthritic joints, compared with corresponding regions in control joints. Cartilage from the palmar aspect of diseased joints had the highest histologic scores of osteoarthritic sites or of either region in control joints. A different set of genes for dorsal and palmar osteoarthritis was identified for high and low gene expression. Articular cartilage from the dorsal region had surface fraying and greater expression of genes coding for collagen matrix components and proteins with anti-apoptotic function, compared with control specimens. Articular cartilage from the palmar region had greater fraying, deep fissures, and less expression of genes coding for glycosaminoglycan matrix formation and proteins with anti-apoptotic function, compared with cartilage from disease-free joints and the dorsal aspect of affected joints. CONCLUSIONS AND CLINICAL RELEVANCE: Metacarpal condyles of horses with naturally occurring osteoarthritis had an identifiable and regional gene expression signature with typical morphologic features. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/16881840/Gross_histologic_and_gene_expression_characteristics_of_osteoarthritic_articular_cartilage_of_the_metacarpal_condyle_of_horses_ L2 - http://avmajournals.avma.org/doi/full/10.2460/ajvr.67.8.1299?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -