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Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water.
J Appl Microbiol. 2006 Aug; 101(2):453-63.JA

Abstract

AIM

To determine whether batch solar disinfection (SODIS) can be used to inactivate oocysts of Cryptosporidium parvum and cysts of Giardia muris in experimentally contaminated water.

METHODS AND RESULTS

Suspensions of oocysts and cysts were exposed to simulated global solar irradiation of 830 W m(-2) for different exposure times at a constant temperature of 40 degrees C. Infectivity tests were carried out using CD-1 suckling mice in the Cryptosporidium experiments and newly weaned CD-1 mice in the Giardia experiments. Exposure times of > or =10 h (total optical dose c. 30 kJ) rendered C. parvum oocysts noninfective. Giardia muris cysts were rendered completely noninfective within 4 h (total optical dose >12 kJ). Scanning electron microscopy and viability (4',6-diamidino-2-phenylindole/propidium iodide fluorogenic dyes and excystation) studies on oocysts of C. parvum suggest that inactivation is caused by damage to the oocyst wall.

CONCLUSIONS

Results show that cysts of G. muris and oocysts of C. parvum are rendered completely noninfective after batch SODIS exposures of 4 and 10 h (respectively) and is also likely to be effective against waterborne cysts of Giardia lamblia.

SIGNIFICANCE AND IMPACT OF THE STUDY

These results demonstrate that SODIS is an appropriate household water treatment technology for use as an emergency intervention in aftermath of natural or man-made disasters against not only bacterial but also protozoan pathogens.

Authors+Show Affiliations

Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland. kmcguigan@rcsi.ieNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16882154

Citation

McGuigan, K G., et al. "Batch Solar Disinfection Inactivates Oocysts of Cryptosporidium Parvum and Cysts of Giardia Muris in Drinking Water." Journal of Applied Microbiology, vol. 101, no. 2, 2006, pp. 453-63.
McGuigan KG, Méndez-Hermida F, Castro-Hermida JA, et al. Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water. J Appl Microbiol. 2006;101(2):453-63.
McGuigan, K. G., Méndez-Hermida, F., Castro-Hermida, J. A., Ares-Mazás, E., Kehoe, S. C., Boyle, M., Sichel, C., Fernández-Ibáñez, P., Meyer, B. P., Ramalingham, S., & Meyer, E. A. (2006). Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water. Journal of Applied Microbiology, 101(2), 453-63.
McGuigan KG, et al. Batch Solar Disinfection Inactivates Oocysts of Cryptosporidium Parvum and Cysts of Giardia Muris in Drinking Water. J Appl Microbiol. 2006;101(2):453-63. PubMed PMID: 16882154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Batch solar disinfection inactivates oocysts of Cryptosporidium parvum and cysts of Giardia muris in drinking water. AU - McGuigan,K G, AU - Méndez-Hermida,F, AU - Castro-Hermida,J A, AU - Ares-Mazás,E, AU - Kehoe,S C, AU - Boyle,M, AU - Sichel,C, AU - Fernández-Ibáñez,P, AU - Meyer,B P, AU - Ramalingham,S, AU - Meyer,E A, PY - 2006/8/3/pubmed PY - 2007/3/30/medline PY - 2006/8/3/entrez SP - 453 EP - 63 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 101 IS - 2 N2 - AIM: To determine whether batch solar disinfection (SODIS) can be used to inactivate oocysts of Cryptosporidium parvum and cysts of Giardia muris in experimentally contaminated water. METHODS AND RESULTS: Suspensions of oocysts and cysts were exposed to simulated global solar irradiation of 830 W m(-2) for different exposure times at a constant temperature of 40 degrees C. Infectivity tests were carried out using CD-1 suckling mice in the Cryptosporidium experiments and newly weaned CD-1 mice in the Giardia experiments. Exposure times of > or =10 h (total optical dose c. 30 kJ) rendered C. parvum oocysts noninfective. Giardia muris cysts were rendered completely noninfective within 4 h (total optical dose >12 kJ). Scanning electron microscopy and viability (4',6-diamidino-2-phenylindole/propidium iodide fluorogenic dyes and excystation) studies on oocysts of C. parvum suggest that inactivation is caused by damage to the oocyst wall. CONCLUSIONS: Results show that cysts of G. muris and oocysts of C. parvum are rendered completely noninfective after batch SODIS exposures of 4 and 10 h (respectively) and is also likely to be effective against waterborne cysts of Giardia lamblia. SIGNIFICANCE AND IMPACT OF THE STUDY: These results demonstrate that SODIS is an appropriate household water treatment technology for use as an emergency intervention in aftermath of natural or man-made disasters against not only bacterial but also protozoan pathogens. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/16882154/Batch_solar_disinfection_inactivates_oocysts_of_Cryptosporidium_parvum_and_cysts_of_Giardia_muris_in_drinking_water_ L2 - https://doi.org/10.1111/j.1365-2672.2006.02935.x DB - PRIME DP - Unbound Medicine ER -