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Structural characterisation of novel lichen heteroglycans by NMR spectroscopy and methylation analysis.
Carbohydr Res. 2006 Oct 16; 341(14):2449-55.CR

Abstract

Two galactofuranomannans, Ths-4 and Ths-5, were isolated from the lichen, Thamnolia vermicularis var. subuliformis, using ethanol fractionation and anion-exchange and size-exclusion chromatography. The average molecular weights of Ths-4 and Ths-5 were estimated to be 19 and 200 kDa, respectively. Structural characterisation of Ths-4, Ths-5 and their partially hydrolysed derivatives was performed by methanolysis and methylation analysis. The intact and partially hydrolysed Ths-4 was further analysed using NMR spectroscopy (1D, COSY, NOESY, TOCSY, HSQC and HMBC). According to the data obtained, the heteroglycans Ths-4 and Ths-5 have similar structures, but have large differences in molecular weight. The structure is composed of 3-O-linked and 5-O-linked galactofuranosyl chains linked to a mannan core. The mannan core consists of a main chain of alpha-(1-->6)-linked mannopyranosyl residues, substituted at O-2 with either a single alpha-mannopyranosyl unit or an alpha-Manp-(1-->2)-alpha-Manp-(1-->2)-alpha-Manp group in the ratio of approximately 1:3, respectively. The polysaccharides have idealised repeating blocks as is shown.

Authors+Show Affiliations

Omarsdottir S
University of Iceland, Faculty of Pharmacy, Hagi, Hofsvallagata 53, IS-107 Reykjavik, Iceland.
Petersen BO
No affiliation info available
Paulsen BS
No affiliation info available
Togola A
No affiliation info available
Duus JØ
No affiliation info available
Olafsdottir ES
No affiliation info available

MeSH

Chromatography, Ion ExchangeGlucansLichensMagnetic Resonance SpectroscopyMethylationPlant PreparationsPolysaccharides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16884705

Citation

Omarsdottir, Sesselja, et al. "Structural Characterisation of Novel Lichen Heteroglycans By NMR Spectroscopy and Methylation Analysis." Carbohydrate Research, vol. 341, no. 14, 2006, pp. 2449-55.
Omarsdottir S, Petersen BO, Paulsen BS, et al. Structural characterisation of novel lichen heteroglycans by NMR spectroscopy and methylation analysis. Carbohydr Res. 2006;341(14):2449-55.
Omarsdottir, S., Petersen, B. O., Paulsen, B. S., Togola, A., Duus, J. Ø., & Olafsdottir, E. S. (2006). Structural characterisation of novel lichen heteroglycans by NMR spectroscopy and methylation analysis. Carbohydrate Research, 341(14), 2449-55.
Omarsdottir S, et al. Structural Characterisation of Novel Lichen Heteroglycans By NMR Spectroscopy and Methylation Analysis. Carbohydr Res. 2006 Oct 16;341(14):2449-55. PubMed PMID: 16884705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural characterisation of novel lichen heteroglycans by NMR spectroscopy and methylation analysis. AU - Omarsdottir,Sesselja, AU - Petersen,Bent O, AU - Paulsen,Berit Smestad, AU - Togola,Adiaratou, AU - Duus,Jens Ø, AU - Olafsdottir,Elin S, Y1 - 2006/08/01/ PY - 2006/03/16/received PY - 2006/06/08/revised PY - 2006/06/27/accepted PY - 2006/8/4/pubmed PY - 2007/2/14/medline PY - 2006/8/4/entrez SP - 2449 EP - 55 JF - Carbohydrate research JO - Carbohydr Res VL - 341 IS - 14 N2 - Two galactofuranomannans, Ths-4 and Ths-5, were isolated from the lichen, Thamnolia vermicularis var. subuliformis, using ethanol fractionation and anion-exchange and size-exclusion chromatography. The average molecular weights of Ths-4 and Ths-5 were estimated to be 19 and 200 kDa, respectively. Structural characterisation of Ths-4, Ths-5 and their partially hydrolysed derivatives was performed by methanolysis and methylation analysis. The intact and partially hydrolysed Ths-4 was further analysed using NMR spectroscopy (1D, COSY, NOESY, TOCSY, HSQC and HMBC). According to the data obtained, the heteroglycans Ths-4 and Ths-5 have similar structures, but have large differences in molecular weight. The structure is composed of 3-O-linked and 5-O-linked galactofuranosyl chains linked to a mannan core. The mannan core consists of a main chain of alpha-(1-->6)-linked mannopyranosyl residues, substituted at O-2 with either a single alpha-mannopyranosyl unit or an alpha-Manp-(1-->2)-alpha-Manp-(1-->2)-alpha-Manp group in the ratio of approximately 1:3, respectively. The polysaccharides have idealised repeating blocks as is shown. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/16884705/Structural_characterisation_of_novel_lichen_heteroglycans_by_NMR_spectroscopy_and_methylation_analysis_ DB - PRIME DP - Unbound Medicine ER -