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Fast and precise protein tracking using repeated reversible photoactivation.
Traffic. 2006 Oct; 7(10):1304-10.T

Abstract

Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.

Authors+Show Affiliations

Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia Evrogen JSC, Miklukho-Maklaya 16/10, Moscow 117997, Russia. chudakovdm@mail.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16889652

Citation

Chudakov, Dmitriy M., et al. "Fast and Precise Protein Tracking Using Repeated Reversible Photoactivation." Traffic (Copenhagen, Denmark), vol. 7, no. 10, 2006, pp. 1304-10.
Chudakov DM, Chepurnykh TV, Belousov VV, et al. Fast and precise protein tracking using repeated reversible photoactivation. Traffic. 2006;7(10):1304-10.
Chudakov, D. M., Chepurnykh, T. V., Belousov, V. V., Lukyanov, S., & Lukyanov, K. A. (2006). Fast and precise protein tracking using repeated reversible photoactivation. Traffic (Copenhagen, Denmark), 7(10), 1304-10.
Chudakov DM, et al. Fast and Precise Protein Tracking Using Repeated Reversible Photoactivation. Traffic. 2006;7(10):1304-10. PubMed PMID: 16889652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast and precise protein tracking using repeated reversible photoactivation. AU - Chudakov,Dmitriy M, AU - Chepurnykh,Tatyana V, AU - Belousov,Vsevolod V, AU - Lukyanov,Sergey, AU - Lukyanov,Konstantin A, Y1 - 2006/08/02/ PY - 2006/8/8/pubmed PY - 2007/5/9/medline PY - 2006/8/8/entrez SP - 1304 EP - 10 JF - Traffic (Copenhagen, Denmark) JO - Traffic VL - 7 IS - 10 N2 - Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events. SN - 1398-9219 UR - https://www.unboundmedicine.com/medline/citation/16889652/Fast_and_precise_protein_tracking_using_repeated_reversible_photoactivation_ L2 - https://doi.org/10.1111/j.1600-0854.2006.00468.x DB - PRIME DP - Unbound Medicine ER -