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Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study.
Clin Chim Acta. 2007 Jan; 375(1-2):82-6.CC

Abstract

BACKGROUND

Oxidative stress, resulting from an imbalance between prooxidant and antioxidant systems in favor of the former, largely contributes to immune system deregulation and complications observed in end-stage renal disease (ESRD) and patients treated with hemodialysis. Reactive oxygen species and free radicals are involved in the nephrotoxicity induced by a synthetic anticancer drug cisplatin.

METHODS

A comparative study on the nephroprotective effects of antioxidant vitamins (250 and 500 mg/kg, p.o.), vitamin C (ascorbic acid) and vitamin E (alpha-tocopherol), was evaluated using cisplatin (10 mg/kg body wt, i.p.) induced oxidative renal damage in mice. Urea and creatinine in serum were estimated for the renal function. Antioxidant status was estimated in kidney homogenate.

RESULTS

We found that both vitamins at 500 mg/kg significantly (P<0.01) protected the nephrotoxicity induced by cisplatin. The cisplatin induced increase of urea and creatinine concentrations were reduced in the vitamins plus cisplatin (250 and 500 mg/kg, p.o.)-treated groups. However the cisplatin induced decline of renal antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities were increased only in the 500 mg/kg vitamins treated groups. Both vitamins at 250 and 500 mg/kg could increase the concentration of reduced glutathione (GSH) and protected the increase of cisplatin induced lipid peroxidation.

CONCLUSIONS

Higher doses of vitamins are effective to protect oxidative renal damage and vitamin C is the better nephroprotective agent than vitamin E. The protection is mediated partially by preventing the decline of renal antioxidant status.

Authors+Show Affiliations

Department of Biochemistry, Amala Institute of Medical Sciences, Amala Nagar, Thrissur, Kerala, 680 555, India. taajith@rediffmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16889761

Citation

Ajith, T A., et al. "Ascorbic Acid and Alpha-tocopherol Protect Anticancer Drug Cisplatin Induced Nephrotoxicity in Mice: a Comparative Study." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 375, no. 1-2, 2007, pp. 82-6.
Ajith TA, Usha S, Nivitha V. Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clin Chim Acta. 2007;375(1-2):82-6.
Ajith, T. A., Usha, S., & Nivitha, V. (2007). Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. Clinica Chimica Acta; International Journal of Clinical Chemistry, 375(1-2), 82-6.
Ajith TA, Usha S, Nivitha V. Ascorbic Acid and Alpha-tocopherol Protect Anticancer Drug Cisplatin Induced Nephrotoxicity in Mice: a Comparative Study. Clin Chim Acta. 2007;375(1-2):82-6. PubMed PMID: 16889761.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ascorbic acid and alpha-tocopherol protect anticancer drug cisplatin induced nephrotoxicity in mice: a comparative study. AU - Ajith,T A, AU - Usha,S, AU - Nivitha,V, Y1 - 2006/06/14/ PY - 2006/04/01/received PY - 2006/06/03/revised PY - 2006/06/11/accepted PY - 2006/8/8/pubmed PY - 2007/2/3/medline PY - 2006/8/8/entrez SP - 82 EP - 6 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 375 IS - 1-2 N2 - BACKGROUND: Oxidative stress, resulting from an imbalance between prooxidant and antioxidant systems in favor of the former, largely contributes to immune system deregulation and complications observed in end-stage renal disease (ESRD) and patients treated with hemodialysis. Reactive oxygen species and free radicals are involved in the nephrotoxicity induced by a synthetic anticancer drug cisplatin. METHODS: A comparative study on the nephroprotective effects of antioxidant vitamins (250 and 500 mg/kg, p.o.), vitamin C (ascorbic acid) and vitamin E (alpha-tocopherol), was evaluated using cisplatin (10 mg/kg body wt, i.p.) induced oxidative renal damage in mice. Urea and creatinine in serum were estimated for the renal function. Antioxidant status was estimated in kidney homogenate. RESULTS: We found that both vitamins at 500 mg/kg significantly (P<0.01) protected the nephrotoxicity induced by cisplatin. The cisplatin induced increase of urea and creatinine concentrations were reduced in the vitamins plus cisplatin (250 and 500 mg/kg, p.o.)-treated groups. However the cisplatin induced decline of renal antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities were increased only in the 500 mg/kg vitamins treated groups. Both vitamins at 250 and 500 mg/kg could increase the concentration of reduced glutathione (GSH) and protected the increase of cisplatin induced lipid peroxidation. CONCLUSIONS: Higher doses of vitamins are effective to protect oxidative renal damage and vitamin C is the better nephroprotective agent than vitamin E. The protection is mediated partially by preventing the decline of renal antioxidant status. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/16889761/Ascorbic_acid_and_alpha_tocopherol_protect_anticancer_drug_cisplatin_induced_nephrotoxicity_in_mice:_a_comparative_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-8981(06)00387-1 DB - PRIME DP - Unbound Medicine ER -