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Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections.
J Clin Microbiol. 2006 Aug; 44(8):2872-8.JC

Abstract

Rapid diagnosis and serotyping of dengue virus (DV) infections are important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. However, the speed and accuracy of diagnosis must be balanced against test cost and availability, especially in developing countries. We developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for early detection and serotyping of DV serotype 1 (DV1) by using well-characterized monoclonal antibodies (MAbs) specific to nonstructural protein 1 (NS1) of DV1. With this assay, a total of 462 serum specimens from clinically probable DV1-infected patients during the DV1 epidemic in Guangdong, China, in 2002 and 2003 were analyzed. DV1 NS1 was detectable in blood circulation from the first day up to day 18 after onset of symptoms, with a peak at days 6 to 10. The sensitivity of DV1 NS1 detection in serum specimens with reference to results from reverse transcriptase PCR was 82%, and the specificity was 98.9% with reference to 469 healthy blood donors. No cross-reactions with any of the other three DV serotypes or other closely related members of the genus Flavivirus (Japanese encephalitis virus and Yellow fever virus) were observed when tested with the clinical specimens or virus cultures. These findings suggest that the serotype-specific MAb-based NS1 antigen capture ELISA may be a valuable tool for early diagnosis and serotyping of DV infections, while also providing a standardized assay for the analysis of a great number of clinical samples with convenience and cost-effectiveness.

Authors+Show Affiliations

Center of Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16891505

Citation

Xu, Hua, et al. "Serotype 1-specific Monoclonal Antibody-based Antigen Capture Immunoassay for Detection of Circulating Nonstructural Protein NS1: Implications for Early Diagnosis and Serotyping of Dengue Virus Infections." Journal of Clinical Microbiology, vol. 44, no. 8, 2006, pp. 2872-8.
Xu H, Di B, Pan YX, et al. Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections. J Clin Microbiol. 2006;44(8):2872-8.
Xu, H., Di, B., Pan, Y. X., Qiu, L. W., Wang, Y. D., Hao, W., He, L. J., Yuen, K. Y., & Che, X. Y. (2006). Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections. Journal of Clinical Microbiology, 44(8), 2872-8.
Xu H, et al. Serotype 1-specific Monoclonal Antibody-based Antigen Capture Immunoassay for Detection of Circulating Nonstructural Protein NS1: Implications for Early Diagnosis and Serotyping of Dengue Virus Infections. J Clin Microbiol. 2006;44(8):2872-8. PubMed PMID: 16891505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotype 1-specific monoclonal antibody-based antigen capture immunoassay for detection of circulating nonstructural protein NS1: Implications for early diagnosis and serotyping of dengue virus infections. AU - Xu,Hua, AU - Di,Biao, AU - Pan,Yu-xian, AU - Qiu,Li-wen, AU - Wang,Ya-di, AU - Hao,Wei, AU - He,Li-juan, AU - Yuen,Kwok-yung, AU - Che,Xiao-yan, PY - 2006/8/8/pubmed PY - 2006/11/3/medline PY - 2006/8/8/entrez SP - 2872 EP - 8 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 44 IS - 8 N2 - Rapid diagnosis and serotyping of dengue virus (DV) infections are important for timely clinical management and epidemiological control in areas where multiple flaviviruses are endemic. However, the speed and accuracy of diagnosis must be balanced against test cost and availability, especially in developing countries. We developed a specific antigen capture enzyme-linked immunosorbent assay (ELISA) for early detection and serotyping of DV serotype 1 (DV1) by using well-characterized monoclonal antibodies (MAbs) specific to nonstructural protein 1 (NS1) of DV1. With this assay, a total of 462 serum specimens from clinically probable DV1-infected patients during the DV1 epidemic in Guangdong, China, in 2002 and 2003 were analyzed. DV1 NS1 was detectable in blood circulation from the first day up to day 18 after onset of symptoms, with a peak at days 6 to 10. The sensitivity of DV1 NS1 detection in serum specimens with reference to results from reverse transcriptase PCR was 82%, and the specificity was 98.9% with reference to 469 healthy blood donors. No cross-reactions with any of the other three DV serotypes or other closely related members of the genus Flavivirus (Japanese encephalitis virus and Yellow fever virus) were observed when tested with the clinical specimens or virus cultures. These findings suggest that the serotype-specific MAb-based NS1 antigen capture ELISA may be a valuable tool for early diagnosis and serotyping of DV infections, while also providing a standardized assay for the analysis of a great number of clinical samples with convenience and cost-effectiveness. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/16891505/Serotype_1_specific_monoclonal_antibody_based_antigen_capture_immunoassay_for_detection_of_circulating_nonstructural_protein_NS1:_Implications_for_early_diagnosis_and_serotyping_of_dengue_virus_infections_ DB - PRIME DP - Unbound Medicine ER -