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Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis.
J Clin Microbiol. 2006 Aug; 44(8):3028-30.JC

Abstract

A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.

Links

Publisher Full Text
ncbi.nlm.nih.gov
jcm.asm.org
PMC Free PDF

Authors+Show Affiliations

Meumann EM
Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.
Novak RT
No affiliation info available
Gal D
No affiliation info available
Kaestli ME
No affiliation info available
Mayo M
No affiliation info available
Hanson JP
No affiliation info available
Spencer E
No affiliation info available
Glass MB
No affiliation info available
Gee JE
No affiliation info available
Wilkins PP
No affiliation info available
Currie BJ
No affiliation info available

MeSH

Biological TransportBloodBody FluidsBurkholderia pseudomalleiDNA, BacterialEndemic DiseasesHumansMelioidosisPolymerase Chain ReactionSensitivity and SpecificitySepsis

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16891534

Citation

Meumann, Ella M., et al. "Clinical Evaluation of a Type III Secretion System Real-time PCR Assay for Diagnosing Melioidosis." Journal of Clinical Microbiology, vol. 44, no. 8, 2006, pp. 3028-30.
Meumann EM, Novak RT, Gal D, et al. Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis. J Clin Microbiol. 2006;44(8):3028-30.
Meumann, E. M., Novak, R. T., Gal, D., Kaestli, M. E., Mayo, M., Hanson, J. P., Spencer, E., Glass, M. B., Gee, J. E., Wilkins, P. P., & Currie, B. J. (2006). Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis. Journal of Clinical Microbiology, 44(8), 3028-30.
Meumann EM, et al. Clinical Evaluation of a Type III Secretion System Real-time PCR Assay for Diagnosing Melioidosis. J Clin Microbiol. 2006;44(8):3028-30. PubMed PMID: 16891534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical evaluation of a type III secretion system real-time PCR assay for diagnosing melioidosis. AU - Meumann,Ella M, AU - Novak,Ryan T, AU - Gal,Daniel, AU - Kaestli,Mirjam E, AU - Mayo,Mark, AU - Hanson,Joshua P, AU - Spencer,Emma, AU - Glass,Mindy B, AU - Gee,Jay E, AU - Wilkins,Patricia P, AU - Currie,Bart J, PY - 2006/8/8/pubmed PY - 2006/11/3/medline PY - 2006/8/8/entrez SP - 3028 EP - 30 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 44 IS - 8 N2 - A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/16891534/Clinical_evaluation_of_a_type_III_secretion_system_real_time_PCR_assay_for_diagnosing_melioidosis_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=16891534 DB - PRIME DP - Unbound Medicine ER -