Tags

Type your tag names separated by a space and hit enter

An anthocyanin/polyphenolic-rich fruit juice reduces oxidative DNA damage and increases glutathione level in healthy probands.
Biotechnol J 2006; 1(4):388-97BJ

Abstract

Oxidative cell damage is involved in the pathogenesis of atherosclerosis, cancer, diabetes and other diseases. Uptake of fruit juice with especially high content of antioxidant flavonoids/polyphenols, might reduce oxidative cell damage. Therefore, an intervention study was performed with a red mixed berry juice [trolox equivalent antioxidative capacity (TEAC): 19.1 mmol/L trolox] and a corresponding polyphenol-depleted juice (polyphenols largely removed, TEAC 2.4 mmol/L trolox), serving as control. After a 3-week run-in period, 18 male probands daily consumed 700 mL juice, and 9 consumed control juice, in a 4-week intervention, followed by a 3-week wash-out. Samples were collected weekly to analyze DNA damage (comet assay), lipid peroxidation (plasma malondialdehyde: HPLC/fluorescence; urinary isoprostanes: GC-MS), blood glutathione (photometrically), DNA-binding activity of nuclear factor-kappaB (ELISA) and plasma carotenoid/alpha-tocopherol levels (HPLC-DAD). During intervention with the fruit juice, a decrease of oxidative DNA damage (p<5x10(-4)) and an increase of reduced glutathione (p<5x10(-4)) and of glutathione status (p<0.05) were observed, which returned to the run-in levels in the subsequent wash-out phase. The other biomarkers were not significantly modulated by the juice supplement. Intervention with the control juice did not result in reduction of oxidative damage. In conclusion, the fruit juice clearly reduces oxidative cell damage in healthy probands.

Authors+Show Affiliations

Department of Chemistry, Division of Food Chemistry and Environmental Toxicology, University of Kaiserslautern, Kaiserslautern, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16892265

Citation

Weisel, Tamara, et al. "An Anthocyanin/polyphenolic-rich Fruit Juice Reduces Oxidative DNA Damage and Increases Glutathione Level in Healthy Probands." Biotechnology Journal, vol. 1, no. 4, 2006, pp. 388-97.
Weisel T, Baum M, Eisenbrand G, et al. An anthocyanin/polyphenolic-rich fruit juice reduces oxidative DNA damage and increases glutathione level in healthy probands. Biotechnol J. 2006;1(4):388-97.
Weisel, T., Baum, M., Eisenbrand, G., Dietrich, H., Will, F., Stockis, J. P., ... Janzowski, C. (2006). An anthocyanin/polyphenolic-rich fruit juice reduces oxidative DNA damage and increases glutathione level in healthy probands. Biotechnology Journal, 1(4), pp. 388-97.
Weisel T, et al. An Anthocyanin/polyphenolic-rich Fruit Juice Reduces Oxidative DNA Damage and Increases Glutathione Level in Healthy Probands. Biotechnol J. 2006;1(4):388-97. PubMed PMID: 16892265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An anthocyanin/polyphenolic-rich fruit juice reduces oxidative DNA damage and increases glutathione level in healthy probands. AU - Weisel,Tamara, AU - Baum,Matthias, AU - Eisenbrand,Gerhard, AU - Dietrich,Helmut, AU - Will,Frank, AU - Stockis,Jean-Pierre, AU - Kulling,Sabine, AU - Rüfer,Corinna, AU - Johannes,Christian, AU - Janzowski,Christine, PY - 2006/8/8/pubmed PY - 2006/10/25/medline PY - 2006/8/8/entrez SP - 388 EP - 97 JF - Biotechnology journal JO - Biotechnol J VL - 1 IS - 4 N2 - Oxidative cell damage is involved in the pathogenesis of atherosclerosis, cancer, diabetes and other diseases. Uptake of fruit juice with especially high content of antioxidant flavonoids/polyphenols, might reduce oxidative cell damage. Therefore, an intervention study was performed with a red mixed berry juice [trolox equivalent antioxidative capacity (TEAC): 19.1 mmol/L trolox] and a corresponding polyphenol-depleted juice (polyphenols largely removed, TEAC 2.4 mmol/L trolox), serving as control. After a 3-week run-in period, 18 male probands daily consumed 700 mL juice, and 9 consumed control juice, in a 4-week intervention, followed by a 3-week wash-out. Samples were collected weekly to analyze DNA damage (comet assay), lipid peroxidation (plasma malondialdehyde: HPLC/fluorescence; urinary isoprostanes: GC-MS), blood glutathione (photometrically), DNA-binding activity of nuclear factor-kappaB (ELISA) and plasma carotenoid/alpha-tocopherol levels (HPLC-DAD). During intervention with the fruit juice, a decrease of oxidative DNA damage (p<5x10(-4)) and an increase of reduced glutathione (p<5x10(-4)) and of glutathione status (p<0.05) were observed, which returned to the run-in levels in the subsequent wash-out phase. The other biomarkers were not significantly modulated by the juice supplement. Intervention with the control juice did not result in reduction of oxidative damage. In conclusion, the fruit juice clearly reduces oxidative cell damage in healthy probands. SN - 1860-6768 UR - https://www.unboundmedicine.com/medline/citation/16892265/An_anthocyanin/polyphenolic_rich_fruit_juice_reduces_oxidative_DNA_damage_and_increases_glutathione_level_in_healthy_probands_ L2 - https://doi.org/10.1002/biot.200600004 DB - PRIME DP - Unbound Medicine ER -