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Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome.
J Gen Virol. 2006 Sep; 87(Pt 9):2549-62.JG

Abstract

The whole-genome sequence of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) was analysed. The entire nucleotide sequence of the HycuNPV genome was 132 959 bp long, with a G+C content of 45.1 mol%. A total of 148 open reading frames (ORFs) consisting of more than 50 aa were encoded by the genome. HycuNPV shares more than 122 ORFs with other lepidopteran group I NPVs, including Autographa californica MNPV, Bombyx mori NPV, Choristoneura fumiferana MNPV (CfMNPV), Choristoneura fumiferana defective NPV, Epiphyas postvittana MNPV and Orgyia pseudotsugata MNPV (OpMNPV). Six ORFs are identified as being unique to HycuNPV. Most of the HycuNPV ORFs showed higher similarity to CfMNPV and OpMNPV ORFs than to those of the other group I NPVs. HycuNPV encodes two conotoxin-like homologues (ctls), which are observed only in OpMNPV in group I NPVs. HycuNPV encodes three inhibitors of apoptosis (iaps), hycu-iap-1, hycu-iap-2 and hycu-iap-3, a feature that it shares only with CfMNPV. In addition, six homologous regions (hrs) are identified in the HycuNPV genome. These hrs are located in regions similar to those of the OpMNPV hrs, but different from most of the CfMNPV hrs. Based on the close phylogenetic relationship and conservation of group I NPV-specific genes, such as gp64, ie-2 and ptp-1, it is concluded that HycuNPV belongs to the group I NPVs and is most similar to CfMNPV or OpMNPV.

Authors+Show Affiliations

Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16894193

Citation

Ikeda, Motoko, et al. "Gene Organization and Complete Sequence of the Hyphantria Cunea Nucleopolyhedrovirus Genome." The Journal of General Virology, vol. 87, no. Pt 9, 2006, pp. 2549-62.
Ikeda M, Shikata M, Shirata N, et al. Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome. J Gen Virol. 2006;87(Pt 9):2549-62.
Ikeda, M., Shikata, M., Shirata, N., Chaeychomsri, S., & Kobayashi, M. (2006). Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome. The Journal of General Virology, 87(Pt 9), 2549-62.
Ikeda M, et al. Gene Organization and Complete Sequence of the Hyphantria Cunea Nucleopolyhedrovirus Genome. J Gen Virol. 2006;87(Pt 9):2549-62. PubMed PMID: 16894193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome. AU - Ikeda,Motoko, AU - Shikata,Masamitsu, AU - Shirata,Noriko, AU - Chaeychomsri,Sudawan, AU - Kobayashi,Michihiro, PY - 2006/8/9/pubmed PY - 2006/9/22/medline PY - 2006/8/9/entrez SP - 2549 EP - 62 JF - The Journal of general virology JO - J. Gen. Virol. VL - 87 IS - Pt 9 N2 - The whole-genome sequence of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) was analysed. The entire nucleotide sequence of the HycuNPV genome was 132 959 bp long, with a G+C content of 45.1 mol%. A total of 148 open reading frames (ORFs) consisting of more than 50 aa were encoded by the genome. HycuNPV shares more than 122 ORFs with other lepidopteran group I NPVs, including Autographa californica MNPV, Bombyx mori NPV, Choristoneura fumiferana MNPV (CfMNPV), Choristoneura fumiferana defective NPV, Epiphyas postvittana MNPV and Orgyia pseudotsugata MNPV (OpMNPV). Six ORFs are identified as being unique to HycuNPV. Most of the HycuNPV ORFs showed higher similarity to CfMNPV and OpMNPV ORFs than to those of the other group I NPVs. HycuNPV encodes two conotoxin-like homologues (ctls), which are observed only in OpMNPV in group I NPVs. HycuNPV encodes three inhibitors of apoptosis (iaps), hycu-iap-1, hycu-iap-2 and hycu-iap-3, a feature that it shares only with CfMNPV. In addition, six homologous regions (hrs) are identified in the HycuNPV genome. These hrs are located in regions similar to those of the OpMNPV hrs, but different from most of the CfMNPV hrs. Based on the close phylogenetic relationship and conservation of group I NPV-specific genes, such as gp64, ie-2 and ptp-1, it is concluded that HycuNPV belongs to the group I NPVs and is most similar to CfMNPV or OpMNPV. SN - 0022-1317 UR - https://www.unboundmedicine.com/medline/citation/16894193/Gene_organization_and_complete_sequence_of_the_Hyphantria_cunea_nucleopolyhedrovirus_genome_ L2 - http://jgv.microbiologyresearch.org/pubmed/content/journal/jgv/10.1099/vir.0.81930-0 DB - PRIME DP - Unbound Medicine ER -