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Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii.
Theor Appl Genet. 2006 Oct; 113(6):1049-62.TA

Abstract

Hexaploid wheat (Triticum aestivum L.) originated about 8,000 years ago from the hybridization of tetraploid wheat with diploid Aegilops tauschii Coss. containing the D-genome. Thus, the bread wheat D-genome is evolutionary young and shows a low degree of polymorphism in the bread wheat gene pool. To increase marker density around the durable leaf rust resistance gene Lr34 located on chromosome 7DS, we used molecular information from the orthologous region in rice. Wheat expressed sequence tags (wESTs) were identified by homology with the rice genes in the interval of interest, but were monomorphic in the 'Arina' x 'Forno' mapping population. To derive new polymorphic markers, bacterial artificial chromosome (BAC) clones representing a total physical size of approximately 1 Mb and belonging to four contigs were isolated from Ae. tauschii by hybridization screening with wheat ESTs. Several BAC clones were low-pass sequenced, resulting in a total of approximately 560 kb of sequence. Ten microsatellite sequences were found, and three of them were polymorphic in our population and were genetically mapped close to Lr34. Comparative analysis of marker order revealed a large inversion between the rice genome and the wheat D-genome. The SWM10 microsatellite is closely linked to Lr34 and has the same allele in the three independent sources of Lr34: 'Frontana', 'Chinese Spring', and 'Forno', as well in most of the genotypes containing Lr34. Therefore, SWM10 is a highly useful marker to assist selection for Lr34 in breeding programs worldwide.

Authors+Show Affiliations

Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, 8008 Zürich, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16896711

Citation

Bossolini, Eligio, et al. "Development of Simple Sequence Repeat Markers Specific for the Lr34 Resistance Region of Wheat Using Sequence Information From Rice and Aegilops Tauschii." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 113, no. 6, 2006, pp. 1049-62.
Bossolini E, Krattinger SG, Keller B. Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theor Appl Genet. 2006;113(6):1049-62.
Bossolini, E., Krattinger, S. G., & Keller, B. (2006). Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 113(6), 1049-62.
Bossolini E, Krattinger SG, Keller B. Development of Simple Sequence Repeat Markers Specific for the Lr34 Resistance Region of Wheat Using Sequence Information From Rice and Aegilops Tauschii. Theor Appl Genet. 2006;113(6):1049-62. PubMed PMID: 16896711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. AU - Bossolini,Eligio, AU - Krattinger,Simon G, AU - Keller,Beat, Y1 - 2006/08/08/ PY - 2006/05/10/received PY - 2006/07/04/accepted PY - 2006/8/10/pubmed PY - 2006/12/27/medline PY - 2006/8/10/entrez SP - 1049 EP - 62 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 113 IS - 6 N2 - Hexaploid wheat (Triticum aestivum L.) originated about 8,000 years ago from the hybridization of tetraploid wheat with diploid Aegilops tauschii Coss. containing the D-genome. Thus, the bread wheat D-genome is evolutionary young and shows a low degree of polymorphism in the bread wheat gene pool. To increase marker density around the durable leaf rust resistance gene Lr34 located on chromosome 7DS, we used molecular information from the orthologous region in rice. Wheat expressed sequence tags (wESTs) were identified by homology with the rice genes in the interval of interest, but were monomorphic in the 'Arina' x 'Forno' mapping population. To derive new polymorphic markers, bacterial artificial chromosome (BAC) clones representing a total physical size of approximately 1 Mb and belonging to four contigs were isolated from Ae. tauschii by hybridization screening with wheat ESTs. Several BAC clones were low-pass sequenced, resulting in a total of approximately 560 kb of sequence. Ten microsatellite sequences were found, and three of them were polymorphic in our population and were genetically mapped close to Lr34. Comparative analysis of marker order revealed a large inversion between the rice genome and the wheat D-genome. The SWM10 microsatellite is closely linked to Lr34 and has the same allele in the three independent sources of Lr34: 'Frontana', 'Chinese Spring', and 'Forno', as well in most of the genotypes containing Lr34. Therefore, SWM10 is a highly useful marker to assist selection for Lr34 in breeding programs worldwide. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16896711/Development_of_simple_sequence_repeat_markers_specific_for_the_Lr34_resistance_region_of_wheat_using_sequence_information_from_rice_and_Aegilops_tauschii_ L2 - https://dx.doi.org/10.1007/s00122-006-0364-5 DB - PRIME DP - Unbound Medicine ER -