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Survival of microencapsulated islets at 400 days posttransplantation in the omental pouch of NOD mice.
Cell Transplant. 2006; 15(4):359-65.CT

Abstract

The long-term durability of agarose microencapsulated islets against autoimmunity was evaluated in NOD mice. Islets were isolated from 6-8-week-old prediabetic male NOD mice and microencapsulated in 5% agarose hydrogel. Microencapsulated or nonencapsulated islets were transplanted into the omental pouch of spontaneously diabetic NOD mice. Although the diabetic NOD mice that received nonencapsulated islets experienced a temporary reversal of their hyperglycemic condition, all 10 of these mice returned to hyperglycemia within 3 weeks. In contrast, 9 of 10 mice transplanted with microencapsulated islets maintained normoglycemia for more than 100 days. Islet grafts were removed at 100, 150, 200, 300, and 400 days posttransplantation. A prompt return to hyperglycemia was observed in the mice after graft removal, indicating that the encapsulated islet grafts were responsible for maintaining euglycemia. Histological examination revealed viable islets in the capsules at all time points of graft removal. In addition, beta-cells within the capsules remained well granulated as revealed by the immunohistochemical detection of insulin. No immune cells were detected inside the microcapsules and no morphological irregularities of the microcapsules were observed at any time point, suggesting that the microcapsules successfully protected the islets from cellular immunity. Sufficient vascularization was evident close to the microcapsules. Considerable numbers of islets showed central necrosis at 400 days posttransplantation, although the necrotic islets made up only a small percentage of the islet grafts. Islets with central necrosis also showed abundant insulin production throughout the entire islets, except for the necrotic part. These results demonstrate the long-term durability of agarose microcapsules against autoimmunity in a syngeneic islet transplantation model in NOD mice.

Authors+Show Affiliations

First Department of Surgery, Nara Medical University, Nara, 634-8522, Japan. tsunehirokobayashi@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16898230

Citation

Kobayashi, Tsunehiro, et al. "Survival of Microencapsulated Islets at 400 Days Posttransplantation in the Omental Pouch of NOD Mice." Cell Transplantation, vol. 15, no. 4, 2006, pp. 359-65.
Kobayashi T, Aomatsu Y, Iwata H, et al. Survival of microencapsulated islets at 400 days posttransplantation in the omental pouch of NOD mice. Cell Transplant. 2006;15(4):359-65.
Kobayashi, T., Aomatsu, Y., Iwata, H., Kin, T., Kanehiro, H., Hisanga, M., Ko, S., Nagao, M., Harb, G., & Nakajima, Y. (2006). Survival of microencapsulated islets at 400 days posttransplantation in the omental pouch of NOD mice. Cell Transplantation, 15(4), 359-65.
Kobayashi T, et al. Survival of Microencapsulated Islets at 400 Days Posttransplantation in the Omental Pouch of NOD Mice. Cell Transplant. 2006;15(4):359-65. PubMed PMID: 16898230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Survival of microencapsulated islets at 400 days posttransplantation in the omental pouch of NOD mice. AU - Kobayashi,Tsunehiro, AU - Aomatsu,Yukio, AU - Iwata,Hiroo, AU - Kin,Tatsuya, AU - Kanehiro,Hiromichi, AU - Hisanga,Michiyoshi, AU - Ko,Saiho, AU - Nagao,Mitsuo, AU - Harb,George, AU - Nakajima,Yoshiyuki, PY - 2006/8/11/pubmed PY - 2006/9/13/medline PY - 2006/8/11/entrez SP - 359 EP - 65 JF - Cell transplantation JO - Cell Transplant VL - 15 IS - 4 N2 - The long-term durability of agarose microencapsulated islets against autoimmunity was evaluated in NOD mice. Islets were isolated from 6-8-week-old prediabetic male NOD mice and microencapsulated in 5% agarose hydrogel. Microencapsulated or nonencapsulated islets were transplanted into the omental pouch of spontaneously diabetic NOD mice. Although the diabetic NOD mice that received nonencapsulated islets experienced a temporary reversal of their hyperglycemic condition, all 10 of these mice returned to hyperglycemia within 3 weeks. In contrast, 9 of 10 mice transplanted with microencapsulated islets maintained normoglycemia for more than 100 days. Islet grafts were removed at 100, 150, 200, 300, and 400 days posttransplantation. A prompt return to hyperglycemia was observed in the mice after graft removal, indicating that the encapsulated islet grafts were responsible for maintaining euglycemia. Histological examination revealed viable islets in the capsules at all time points of graft removal. In addition, beta-cells within the capsules remained well granulated as revealed by the immunohistochemical detection of insulin. No immune cells were detected inside the microcapsules and no morphological irregularities of the microcapsules were observed at any time point, suggesting that the microcapsules successfully protected the islets from cellular immunity. Sufficient vascularization was evident close to the microcapsules. Considerable numbers of islets showed central necrosis at 400 days posttransplantation, although the necrotic islets made up only a small percentage of the islet grafts. Islets with central necrosis also showed abundant insulin production throughout the entire islets, except for the necrotic part. These results demonstrate the long-term durability of agarose microcapsules against autoimmunity in a syngeneic islet transplantation model in NOD mice. SN - 0963-6897 UR - https://www.unboundmedicine.com/medline/citation/16898230/Survival_of_microencapsulated_islets_at_400_days_posttransplantation_in_the_omental_pouch_of_NOD_mice_ L2 - https://journals.sagepub.com/doi/10.3727/000000006783981954?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -