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Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne.
J Eur Acad Dermatol Venereol. 2006 Aug; 20(7):846-52.JE

Abstract

BACKGROUND

The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown.

OBJECTIVE

We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated.

METHODS

We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients.

RESULTS

The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10(-6) m oestradiol (E2) at 6 h but enhanced by 10(-6) m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant.

CONCLUSION

Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne.

Authors+Show Affiliations

Department of Dermatology, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, Taiwan. wchen@cgmh.org.twNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16898909

Citation

Chen, W, et al. "Expression of Sex-determining Genes in Human Sebaceous Glands and Their Possible Role in the Pathogenesis of Acne." Journal of the European Academy of Dermatology and Venereology : JEADV, vol. 20, no. 7, 2006, pp. 846-52.
Chen W, Yang CC, Liao CY, et al. Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne. J Eur Acad Dermatol Venereol. 2006;20(7):846-52.
Chen, W., Yang, C. C., Liao, C. Y., Hung, C. L., Tsai, S. J., Chen, K. F., Sheu, H. M., & Zouboulis, C. C. (2006). Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne. Journal of the European Academy of Dermatology and Venereology : JEADV, 20(7), 846-52.
Chen W, et al. Expression of Sex-determining Genes in Human Sebaceous Glands and Their Possible Role in the Pathogenesis of Acne. J Eur Acad Dermatol Venereol. 2006;20(7):846-52. PubMed PMID: 16898909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of sex-determining genes in human sebaceous glands and their possible role in the pathogenesis of acne. AU - Chen,W, AU - Yang,C C, AU - Liao,C Y, AU - Hung,C L, AU - Tsai,S J, AU - Chen,K F, AU - Sheu,H M, AU - Zouboulis,C C, PY - 2006/8/11/pubmed PY - 2007/1/16/medline PY - 2006/8/11/entrez SP - 846 EP - 52 JF - Journal of the European Academy of Dermatology and Venereology : JEADV JO - J Eur Acad Dermatol Venereol VL - 20 IS - 7 N2 - BACKGROUND: The human skin, especially the sebaceous gland, is a steroidogenic organ similar to the gonads and adrenal cortex, possessing all the enzymes required for steroid sex-hormone synthesis and metabolism. Factors regulating cutaneous steroidogenesis associated with disease status remain largely unknown. OBJECTIVE: We hypothesized that transcription factors involved in sex formation and regulation of steroidogenesis in the classical steroidogenic organs are also expressed in the sebaceous glands. Their possible role in the pathogenesis of acne were investigated. METHODS: We used reverse transcription polymerase chain reaction (RT-PCR), in situ hybridization and Western blotting to analyse the expression of SF-1, WT-1, SRY, SOX-9 and DAX-1 mRNAs and their proteins in cultured human sebocytes and the facial skin of acne patients. RESULTS: The in situ hybridization study showed SOX-9 mRNA mainly localized in basal keratinocytes, the basal layer of the sebaceous glands and eccrine glands. Immortalized human sebaceous gland cells (SZ95) expressed mRNA for SOX-9, WT-1 and DAX-1 but not for SF-1 or SRY. The expression of DAX-1 protein was slightly inhibited by 10(-6) m oestradiol (E2) at 6 h but enhanced by 10(-6) m dihydrotestosterone (DHT) at 48 h. The facial expression of SOX-9 seemed to be higher in the acne-prone male patients, while DAX-1 was stronger in subjects without acne, although both were statistically insignificant. CONCLUSION: Our findings confirm the expression of some sex-determining genes in human sebaceous glands. Further studies on a larger patient population including the normal controls are needed to elucidate the functional significance of these transcription factors in the pathogenesis of acne. SN - 0926-9959 UR - https://www.unboundmedicine.com/medline/citation/16898909/Expression_of_sex_determining_genes_in_human_sebaceous_glands_and_their_possible_role_in_the_pathogenesis_of_acne_ L2 - https://doi.org/10.1111/j.1468-3083.2006.01663.x DB - PRIME DP - Unbound Medicine ER -