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K-Ras and microsatellite marker analysis of fine-needle aspirates from intraductal papillary mucinous neoplasms of the pancreas.
Diagn Cytopathol. 2006 Sep; 34(9):605-8.DC

Abstract

Preoperative diagnosis of pancreatic cystic lesions is difficult despite the combination of cytomorphology, radiographic imaging characteristics, and fluid tumor markers such as carcinoembryonic antigen. Intraductal papillary mucinous neoplasms (IPMNs) represent a subset of preinvasive pancreatic cystic neoplasms and are associated with accumulated genetic mutations, especially K-ras and tumor suppressor genes such as p53. Application of molecular techniques to cyst fluid obtained by endoscopic ultrasound guided fine-needle aspiration (EUSFNA) may contribute to preoperative assessment. Sixteen patients with pancreatic cystic lesions had cyst fluid obtained by preoperative pancreatic EUSFNA or intraoperative aspiration. All patients subsequently underwent surgical resection of the pancreas and IPMN was documented in all (6 adenomas, 6 borderline tumors, and 4 carcinomas). DNA was extracted from the cyst fluids and mutational analysis for K-ras point mutations and loss of heterozygosity (LOH) analysis using a preselected panel of genomic loci were performed. LOH was observed in 3 of 4 carcinomas as compared to 4 of 11 adenomas and borderline lesions (1 was QNS). LOH and K-ras mutations were both acquired in 2 of 4 carcinomas and in 1 of 12 adenoma/borderline lesions. Although the study is small, molecular analysis for LOH and K-ras mutations is useful in the preoperative evaluation of cystic pancreatic lesions. Increasing degree of neoplasia appears to correlate with increased genetic abnormality using a panel of selected genomic markers.

Authors+Show Affiliations

Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA. schoedelke@upmc.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16900481

Citation

Schoedel, Karen E., et al. "K-Ras and Microsatellite Marker Analysis of Fine-needle Aspirates From Intraductal Papillary Mucinous Neoplasms of the Pancreas." Diagnostic Cytopathology, vol. 34, no. 9, 2006, pp. 605-8.
Schoedel KE, Finkelstein SD, Ohori NP. K-Ras and microsatellite marker analysis of fine-needle aspirates from intraductal papillary mucinous neoplasms of the pancreas. Diagn Cytopathol. 2006;34(9):605-8.
Schoedel, K. E., Finkelstein, S. D., & Ohori, N. P. (2006). K-Ras and microsatellite marker analysis of fine-needle aspirates from intraductal papillary mucinous neoplasms of the pancreas. Diagnostic Cytopathology, 34(9), 605-8.
Schoedel KE, Finkelstein SD, Ohori NP. K-Ras and Microsatellite Marker Analysis of Fine-needle Aspirates From Intraductal Papillary Mucinous Neoplasms of the Pancreas. Diagn Cytopathol. 2006;34(9):605-8. PubMed PMID: 16900481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - K-Ras and microsatellite marker analysis of fine-needle aspirates from intraductal papillary mucinous neoplasms of the pancreas. AU - Schoedel,Karen E, AU - Finkelstein,Sydney D, AU - Ohori,N Paul, PY - 2006/8/11/pubmed PY - 2006/10/18/medline PY - 2006/8/11/entrez SP - 605 EP - 8 JF - Diagnostic cytopathology JO - Diagn Cytopathol VL - 34 IS - 9 N2 - Preoperative diagnosis of pancreatic cystic lesions is difficult despite the combination of cytomorphology, radiographic imaging characteristics, and fluid tumor markers such as carcinoembryonic antigen. Intraductal papillary mucinous neoplasms (IPMNs) represent a subset of preinvasive pancreatic cystic neoplasms and are associated with accumulated genetic mutations, especially K-ras and tumor suppressor genes such as p53. Application of molecular techniques to cyst fluid obtained by endoscopic ultrasound guided fine-needle aspiration (EUSFNA) may contribute to preoperative assessment. Sixteen patients with pancreatic cystic lesions had cyst fluid obtained by preoperative pancreatic EUSFNA or intraoperative aspiration. All patients subsequently underwent surgical resection of the pancreas and IPMN was documented in all (6 adenomas, 6 borderline tumors, and 4 carcinomas). DNA was extracted from the cyst fluids and mutational analysis for K-ras point mutations and loss of heterozygosity (LOH) analysis using a preselected panel of genomic loci were performed. LOH was observed in 3 of 4 carcinomas as compared to 4 of 11 adenomas and borderline lesions (1 was QNS). LOH and K-ras mutations were both acquired in 2 of 4 carcinomas and in 1 of 12 adenoma/borderline lesions. Although the study is small, molecular analysis for LOH and K-ras mutations is useful in the preoperative evaluation of cystic pancreatic lesions. Increasing degree of neoplasia appears to correlate with increased genetic abnormality using a panel of selected genomic markers. SN - 8755-1039 UR - https://www.unboundmedicine.com/medline/citation/16900481/K_Ras_and_microsatellite_marker_analysis_of_fine_needle_aspirates_from_intraductal_papillary_mucinous_neoplasms_of_the_pancreas_ L2 - https://doi.org/10.1002/dc.20511 DB - PRIME DP - Unbound Medicine ER -