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Generation of a conditional null allele of jumonji.
Genesis. 2006 Sep; 44(9):407-11.G

Abstract

The jumonji (jmj) gene plays important roles in multiple organ development in mouse, including cardiovascular development. Since JMJ is expressed widely during mouse development, it is essential that conditional knockout approaches be employed to ablate JMJ in a tissue-specific manner to identify the cell lineage specific roles of JMJ. In this report, we describe the establishment of a jmj conditional null allele in mice by generating a loxP-flanked (floxed) jmj allele, which allows the in vivo ablation of jmj via Cre recombinase-mediated deletion. Gene targeting was used to introduce loxP sites flanking exon 3 of the jmj allele to mouse embryonic stem cells. Our results indicate that the jmj floxed allele converts to a null allele in a heart-specific manner when embryos homozygous for the floxed jmj allele and carrying the alpha-myosin heavy chain promoter-Cre transgene were analyzed by Southern and Northern blot analyses. Therefore, this mouse line harboring the conditional jmj null allele will provide a valuable tool for deciphering the tissue and cell lineage specific roles of JMJ.

Authors+Show Affiliations

Department of Anatomy, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53706, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16900512

Citation

Mysliwiec, Matthew R., et al. "Generation of a Conditional Null Allele of Jumonji." Genesis (New York, N.Y. : 2000), vol. 44, no. 9, 2006, pp. 407-11.
Mysliwiec MR, Chen J, Powers PA, et al. Generation of a conditional null allele of jumonji. Genesis. 2006;44(9):407-11.
Mysliwiec, M. R., Chen, J., Powers, P. A., Bartley, C. R., Schneider, M. D., & Lee, Y. (2006). Generation of a conditional null allele of jumonji. Genesis (New York, N.Y. : 2000), 44(9), 407-11.
Mysliwiec MR, et al. Generation of a Conditional Null Allele of Jumonji. Genesis. 2006;44(9):407-11. PubMed PMID: 16900512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of a conditional null allele of jumonji. AU - Mysliwiec,Matthew R, AU - Chen,Junqin, AU - Powers,Patricia A, AU - Bartley,Christopher R, AU - Schneider,Michael D, AU - Lee,Youngsook, PY - 2006/8/11/pubmed PY - 2006/10/27/medline PY - 2006/8/11/entrez SP - 407 EP - 11 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 44 IS - 9 N2 - The jumonji (jmj) gene plays important roles in multiple organ development in mouse, including cardiovascular development. Since JMJ is expressed widely during mouse development, it is essential that conditional knockout approaches be employed to ablate JMJ in a tissue-specific manner to identify the cell lineage specific roles of JMJ. In this report, we describe the establishment of a jmj conditional null allele in mice by generating a loxP-flanked (floxed) jmj allele, which allows the in vivo ablation of jmj via Cre recombinase-mediated deletion. Gene targeting was used to introduce loxP sites flanking exon 3 of the jmj allele to mouse embryonic stem cells. Our results indicate that the jmj floxed allele converts to a null allele in a heart-specific manner when embryos homozygous for the floxed jmj allele and carrying the alpha-myosin heavy chain promoter-Cre transgene were analyzed by Southern and Northern blot analyses. Therefore, this mouse line harboring the conditional jmj null allele will provide a valuable tool for deciphering the tissue and cell lineage specific roles of JMJ. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/16900512/Generation_of_a_conditional_null_allele_of_jumonji_ L2 - https://doi.org/10.1002/dvg.20221 DB - PRIME DP - Unbound Medicine ER -