Tags

Type your tag names separated by a space and hit enter

Consequences of low birthweight on urinary excretion of DNA markers of oxidative stress in young men.
Scand J Clin Lab Invest. 2006; 66(5):363-70.SJ

Abstract

OBJECTIVE

Low birthweight (LBW) has been associated with an increased risk of development of type 2 diabetes in adult life. Both type 1 and type 2 diabetes mellitus are characterized by increased oxidative stress. The purpose of this study was to investigate whether young healthy adults born with LBW showed differences in oxidative stress under normal conditions and during the added challenge of a physiological Intralipid infusion.

MATERIAL AND METHODS

Urinary excretion of DNA markers of oxidative stress were analyzed by LC-MS/MS in 19 men (aged 19 years) with LBW and in 19 age matched, normal birthweight (NBW) controls pre- and post a 3-fold increase of plasma free fatty acids.

RESULTS

Mean excretion rates of 8-oxo-guanine (8oxoGua), 8-oxo-guanosine (8oxoGuo), 8-oxo-2'deoxyguanosine (8oxodG), and 1,N6-ethenodeoxyadenosine (epsilon dA) did not statistically differ between subjects with LBW and NBW (66.9 versus 73.9 nmol/15 h, 17.8 versus 18.5 nmol/15 h, 11.9 versus 14.4 nmol/15 h and 44.0 versus 43.2 pmol/15 h, respectively). Furthermore, Intralipid infusion did not affect excretion of DNA adducts in LBW or NBW subjects. Statistically significant correlations were found between body mass index and urinary excretion of 8oxoGua (r = 0.64, p = 0.003) and 8oxoGuo (r = 0.64, p = 0.003) in the LBW group only.

CONCLUSIONS

These findings suggest that oxidative stress may be a consequence of diabetes and is not, or at least only partly, involved in the early pathogenesis of type 2 diabetes.

Authors+Show Affiliations

Department of Clinical Pharmacology Q7642, Rigshospitalet, University Hospital Copenhagen, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

16901847

Citation

Hillestrøm, P R., et al. "Consequences of Low Birthweight On Urinary Excretion of DNA Markers of Oxidative Stress in Young Men." Scandinavian Journal of Clinical and Laboratory Investigation, vol. 66, no. 5, 2006, pp. 363-70.
Hillestrøm PR, Weimann A, Jensen CB, et al. Consequences of low birthweight on urinary excretion of DNA markers of oxidative stress in young men. Scand J Clin Lab Invest. 2006;66(5):363-70.
Hillestrøm, P. R., Weimann, A., Jensen, C. B., Storgaard, H., Vaag, A. A., & Poulsen, H. E. (2006). Consequences of low birthweight on urinary excretion of DNA markers of oxidative stress in young men. Scandinavian Journal of Clinical and Laboratory Investigation, 66(5), 363-70.
Hillestrøm PR, et al. Consequences of Low Birthweight On Urinary Excretion of DNA Markers of Oxidative Stress in Young Men. Scand J Clin Lab Invest. 2006;66(5):363-70. PubMed PMID: 16901847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Consequences of low birthweight on urinary excretion of DNA markers of oxidative stress in young men. AU - Hillestrøm,P R, AU - Weimann,A, AU - Jensen,C B, AU - Storgaard,H, AU - Vaag,A A, AU - Poulsen,H E, PY - 2006/8/12/pubmed PY - 2007/5/30/medline PY - 2006/8/12/entrez SP - 363 EP - 70 JF - Scandinavian journal of clinical and laboratory investigation JO - Scand J Clin Lab Invest VL - 66 IS - 5 N2 - OBJECTIVE: Low birthweight (LBW) has been associated with an increased risk of development of type 2 diabetes in adult life. Both type 1 and type 2 diabetes mellitus are characterized by increased oxidative stress. The purpose of this study was to investigate whether young healthy adults born with LBW showed differences in oxidative stress under normal conditions and during the added challenge of a physiological Intralipid infusion. MATERIAL AND METHODS: Urinary excretion of DNA markers of oxidative stress were analyzed by LC-MS/MS in 19 men (aged 19 years) with LBW and in 19 age matched, normal birthweight (NBW) controls pre- and post a 3-fold increase of plasma free fatty acids. RESULTS: Mean excretion rates of 8-oxo-guanine (8oxoGua), 8-oxo-guanosine (8oxoGuo), 8-oxo-2'deoxyguanosine (8oxodG), and 1,N6-ethenodeoxyadenosine (epsilon dA) did not statistically differ between subjects with LBW and NBW (66.9 versus 73.9 nmol/15 h, 17.8 versus 18.5 nmol/15 h, 11.9 versus 14.4 nmol/15 h and 44.0 versus 43.2 pmol/15 h, respectively). Furthermore, Intralipid infusion did not affect excretion of DNA adducts in LBW or NBW subjects. Statistically significant correlations were found between body mass index and urinary excretion of 8oxoGua (r = 0.64, p = 0.003) and 8oxoGuo (r = 0.64, p = 0.003) in the LBW group only. CONCLUSIONS: These findings suggest that oxidative stress may be a consequence of diabetes and is not, or at least only partly, involved in the early pathogenesis of type 2 diabetes. SN - 0036-5513 UR - https://www.unboundmedicine.com/medline/citation/16901847/Consequences_of_low_birthweight_on_urinary_excretion_of_DNA_markers_of_oxidative_stress_in_young_men_ L2 - https://www.tandfonline.com/doi/full/10.1080/00365510600696402 DB - PRIME DP - Unbound Medicine ER -