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Montelukast prevents the decrease of interleukin-10 and inhibits NF-kappaB activation in inflammatory airway of asthmatic guinea pigs.
Can J Physiol Pharmacol. 2006 May; 84(5):531-7.CJ

Abstract

Interleukin (IL)-10 is an important immunoregulatory and anti-inflammatory cytokine, whereas nuclear factor-kappaB (NF-kappaB) plays an important role in the pathogenesis of asthma. In the present study, the effects of montelukast on the level of IL-10 and on the activation of NF-kappaB in the inflammatory airway of asthmatic guinea pigs were investigated. Guinea pigs were sensitized by ovalbumin. Pulmonary inflammation was observed by hematoxylin and eosin staining. The eosinophils in broncho-alveolar lavage fluid and blood were separated by density gradient centrifugation and counted under microscope. The level of IL-10 in broncho-alveolar lavage fluid was measured by enzyme-linked immunoadsorbent assay. Activation of NF-kappaB in lung tissues was inspected by immunohistochemistry. Montelukast at medium and high doses prevented the decrease of IL-10 in broncho-alveolar lavage fluid (n = 8, p < 0.01 vs. asthma model group), inhibited the activation of NF-kappaB in lung tissues (n = 8; medium dose, p < 0.05; high dose, p < 0.01; vs. asthma model group). There was a significantly negative correlation between the level of IL-10 and the activation of NF-kappaB in lung tissues (r = -0.488, p < 0.01). Montelukast reduced the severity of airway inflammation and the number of eosinophils in asthmatic guinea pigs. From all these findings we conclude that montelukast can prevent the decrease of IL-10 and inhibit the activation of NF-kappaB in inflammatory airway of asthmatic guinea pigs, which may be the new important mechanisms of montelukast's anti-airway-inflammation effects in asthmatic guinea pigs.

Authors+Show Affiliations

Department of Pharmacology, Nanjing Medical University, 210029, Hanzhong Road 140, Nanjing, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16902598

Citation

Wu, Yuqing, et al. "Montelukast Prevents the Decrease of Interleukin-10 and Inhibits NF-kappaB Activation in Inflammatory Airway of Asthmatic Guinea Pigs." Canadian Journal of Physiology and Pharmacology, vol. 84, no. 5, 2006, pp. 531-7.
Wu Y, Zhou C, Tao J, et al. Montelukast prevents the decrease of interleukin-10 and inhibits NF-kappaB activation in inflammatory airway of asthmatic guinea pigs. Can J Physiol Pharmacol. 2006;84(5):531-7.
Wu, Y., Zhou, C., Tao, J., & Li, S. (2006). Montelukast prevents the decrease of interleukin-10 and inhibits NF-kappaB activation in inflammatory airway of asthmatic guinea pigs. Canadian Journal of Physiology and Pharmacology, 84(5), 531-7.
Wu Y, et al. Montelukast Prevents the Decrease of Interleukin-10 and Inhibits NF-kappaB Activation in Inflammatory Airway of Asthmatic Guinea Pigs. Can J Physiol Pharmacol. 2006;84(5):531-7. PubMed PMID: 16902598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Montelukast prevents the decrease of interleukin-10 and inhibits NF-kappaB activation in inflammatory airway of asthmatic guinea pigs. AU - Wu,Yuqing, AU - Zhou,Chenghua, AU - Tao,Jin, AU - Li,Shengnan, PY - 2006/8/12/pubmed PY - 2006/10/27/medline PY - 2006/8/12/entrez SP - 531 EP - 7 JF - Canadian journal of physiology and pharmacology JO - Can J Physiol Pharmacol VL - 84 IS - 5 N2 - Interleukin (IL)-10 is an important immunoregulatory and anti-inflammatory cytokine, whereas nuclear factor-kappaB (NF-kappaB) plays an important role in the pathogenesis of asthma. In the present study, the effects of montelukast on the level of IL-10 and on the activation of NF-kappaB in the inflammatory airway of asthmatic guinea pigs were investigated. Guinea pigs were sensitized by ovalbumin. Pulmonary inflammation was observed by hematoxylin and eosin staining. The eosinophils in broncho-alveolar lavage fluid and blood were separated by density gradient centrifugation and counted under microscope. The level of IL-10 in broncho-alveolar lavage fluid was measured by enzyme-linked immunoadsorbent assay. Activation of NF-kappaB in lung tissues was inspected by immunohistochemistry. Montelukast at medium and high doses prevented the decrease of IL-10 in broncho-alveolar lavage fluid (n = 8, p < 0.01 vs. asthma model group), inhibited the activation of NF-kappaB in lung tissues (n = 8; medium dose, p < 0.05; high dose, p < 0.01; vs. asthma model group). There was a significantly negative correlation between the level of IL-10 and the activation of NF-kappaB in lung tissues (r = -0.488, p < 0.01). Montelukast reduced the severity of airway inflammation and the number of eosinophils in asthmatic guinea pigs. From all these findings we conclude that montelukast can prevent the decrease of IL-10 and inhibit the activation of NF-kappaB in inflammatory airway of asthmatic guinea pigs, which may be the new important mechanisms of montelukast's anti-airway-inflammation effects in asthmatic guinea pigs. SN - 0008-4212 UR - https://www.unboundmedicine.com/medline/citation/16902598/Montelukast_prevents_the_decrease_of_interleukin_10_and_inhibits_NF_kappaB_activation_in_inflammatory_airway_of_asthmatic_guinea_pigs_ L2 - https://cdnsciencepub.com/doi/10.1139/y06-003?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -