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Cloning and characterization of protease inhibitor-like cDNAs from the Hessian fly mayetiola destructor (SAY).
Insect Mol Biol. 2006 Aug; 15(4):485-96.IM

Abstract

Analysis of transcriptomes from the salivary glands and midgut of Hessian fly larvae Mayetiola destructor (say) identified a set of diverse cDNAs that encode proteins with a relatively high percentage (over 10%) of cysteinyl residues. Structural comparison of these putative proteins with known sequences in GenBank revealed that the positions of the cysteinyl residues in the identified proteins were highly conserved within a family of proteinase inhibitors despite very little overall sequence similarity. Phylogenetic analysis sorted this set of cDNAs into five different groups. To determine if these cDNAs indeed encode proteinase inhibitors, recombinant proteins were generated with two cDNAs from two different groups. Biochemical analysis of the recombinant proteins against commercial and insect gut proteinases demonstrated that the recombinant proteins are strong proteinase inhibitors with different specificities. Northern blot and real-time PCR analysis revealed that the different genes were expressed at different developmental stages and in different tissues. The overall results indicated that M. destructor contains a complex of genes that code for proteinase inhibitors which may regulate proteinase activities in different regulatory pathways. The GenBank accession numbers for the cDNAs in this paper were DQ232690 to DQ232718.

Authors+Show Affiliations

Department of Entomology, Kansas State University, Manhattan, KS 66506, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16907835

Citation

Maddur, A A., et al. "Cloning and Characterization of Protease Inhibitor-like cDNAs From the Hessian Fly Mayetiola Destructor (SAY)." Insect Molecular Biology, vol. 15, no. 4, 2006, pp. 485-96.
Maddur AA, Liu X, Zhu YC, et al. Cloning and characterization of protease inhibitor-like cDNAs from the Hessian fly mayetiola destructor (SAY). Insect Mol Biol. 2006;15(4):485-96.
Maddur, A. A., Liu, X., Zhu, Y. C., Fellers, J. P., Oppert, B., Park, Y., Bai, J., Wilde, G. E., & Chen, M. S. (2006). Cloning and characterization of protease inhibitor-like cDNAs from the Hessian fly mayetiola destructor (SAY). Insect Molecular Biology, 15(4), 485-96.
Maddur AA, et al. Cloning and Characterization of Protease Inhibitor-like cDNAs From the Hessian Fly Mayetiola Destructor (SAY). Insect Mol Biol. 2006;15(4):485-96. PubMed PMID: 16907835.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and characterization of protease inhibitor-like cDNAs from the Hessian fly mayetiola destructor (SAY). AU - Maddur,A A, AU - Liu,X, AU - Zhu,Y C, AU - Fellers,J P, AU - Oppert,B, AU - Park,Y, AU - Bai,J, AU - Wilde,G E, AU - Chen,M-S, PY - 2006/8/16/pubmed PY - 2006/12/9/medline PY - 2006/8/16/entrez SP - 485 EP - 96 JF - Insect molecular biology JO - Insect Mol Biol VL - 15 IS - 4 N2 - Analysis of transcriptomes from the salivary glands and midgut of Hessian fly larvae Mayetiola destructor (say) identified a set of diverse cDNAs that encode proteins with a relatively high percentage (over 10%) of cysteinyl residues. Structural comparison of these putative proteins with known sequences in GenBank revealed that the positions of the cysteinyl residues in the identified proteins were highly conserved within a family of proteinase inhibitors despite very little overall sequence similarity. Phylogenetic analysis sorted this set of cDNAs into five different groups. To determine if these cDNAs indeed encode proteinase inhibitors, recombinant proteins were generated with two cDNAs from two different groups. Biochemical analysis of the recombinant proteins against commercial and insect gut proteinases demonstrated that the recombinant proteins are strong proteinase inhibitors with different specificities. Northern blot and real-time PCR analysis revealed that the different genes were expressed at different developmental stages and in different tissues. The overall results indicated that M. destructor contains a complex of genes that code for proteinase inhibitors which may regulate proteinase activities in different regulatory pathways. The GenBank accession numbers for the cDNAs in this paper were DQ232690 to DQ232718. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/16907835/Cloning_and_characterization_of_protease_inhibitor_like_cDNAs_from_the_Hessian_fly_mayetiola_destructor__SAY__ L2 - https://doi.org/10.1111/j.1365-2583.2006.00660.x DB - PRIME DP - Unbound Medicine ER -