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An amperometric uric acid biosensor based on modified Ir-C electrode.
Biosens Bioelectron. 2006 Oct 15; 22(4):482-8.BB

Abstract

The level of uric acid (UA) has a high relationship with gout, hyperuricemia and Lesch-Nyan syndrome. The determination of UA is an important indicator for clinics and diagnoses of kidney failure. An amperometric UA biosensor based on an Ir-modified carbon (Ir-C) working electrode with immobilizing uricase (EC 1.7.3.3) was developed by thick film screen printing technique. This is the first time to report the utilization of an uricase/Ir-C electrode for the determination of UA by using chronoamperometric (CA) method. The high selectivity of UA biosensor was achieved due to the reduction of H(2)O(2) oxidation potential based on Ir-C electrode. Using uricase/Ir-C as the sensing electrode, the interference from the electroactive biological species, such as ascorbic acid (AA) and UA (might be directly oxidized on the sensing electrode) was slight at the sensing potential of 0.25 V (versus Ag/AgCl). UA was detected amperometrically based on uricase/Ir-C electrode with a sensitivity of 16.60 microAmM(-1) over the concentration range of 0.1-0.8 mMUA, which was within the normal range in blood. The detection limit of UA biosensor was 0.01 mM (S/N=6.18) in pH 7 phosphate buffer solution (PBS) at 37 degrees C. The effects of pH, temperature, and enzymatic loading on the sensing characteristics of the UA biosensor were also investigated in this study.

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Authors+Show Affiliations

Luo YC
Center of Nanoscience, Nanotechnology, Department of Chemical Engineering, Tunghai University, Taichung, Taiwan 40704, ROC.
Do JS
No affiliation info available
Liu CC
No affiliation info available

MeSH

Biosensing TechniquesBlood Chemical AnalysisCarbonCoated Materials, BiocompatibleElectrochemistryEquipment DesignEquipment Failure AnalysisIridiumMicroelectrodesReproducibility of ResultsSensitivity and SpecificityUric Acid

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16908130

Citation

Luo, Yung-Chien, et al. "An Amperometric Uric Acid Biosensor Based On Modified Ir-C Electrode." Biosensors & Bioelectronics, vol. 22, no. 4, 2006, pp. 482-8.
Luo YC, Do JS, Liu CC. An amperometric uric acid biosensor based on modified Ir-C electrode. Biosens Bioelectron. 2006;22(4):482-8.
Luo, Y. C., Do, J. S., & Liu, C. C. (2006). An amperometric uric acid biosensor based on modified Ir-C electrode. Biosensors & Bioelectronics, 22(4), 482-8.
Luo YC, Do JS, Liu CC. An Amperometric Uric Acid Biosensor Based On Modified Ir-C Electrode. Biosens Bioelectron. 2006 Oct 15;22(4):482-8. PubMed PMID: 16908130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An amperometric uric acid biosensor based on modified Ir-C electrode. AU - Luo,Yung-Chien, AU - Do,Jing-Shan, AU - Liu,Chung-Chiun, Y1 - 2006/09/05/ PY - 2006/02/13/received PY - 2006/07/01/revised PY - 2006/07/05/accepted PY - 2006/8/16/pubmed PY - 2007/1/18/medline PY - 2006/8/16/entrez SP - 482 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 4 N2 - The level of uric acid (UA) has a high relationship with gout, hyperuricemia and Lesch-Nyan syndrome. The determination of UA is an important indicator for clinics and diagnoses of kidney failure. An amperometric UA biosensor based on an Ir-modified carbon (Ir-C) working electrode with immobilizing uricase (EC 1.7.3.3) was developed by thick film screen printing technique. This is the first time to report the utilization of an uricase/Ir-C electrode for the determination of UA by using chronoamperometric (CA) method. The high selectivity of UA biosensor was achieved due to the reduction of H(2)O(2) oxidation potential based on Ir-C electrode. Using uricase/Ir-C as the sensing electrode, the interference from the electroactive biological species, such as ascorbic acid (AA) and UA (might be directly oxidized on the sensing electrode) was slight at the sensing potential of 0.25 V (versus Ag/AgCl). UA was detected amperometrically based on uricase/Ir-C electrode with a sensitivity of 16.60 microAmM(-1) over the concentration range of 0.1-0.8 mMUA, which was within the normal range in blood. The detection limit of UA biosensor was 0.01 mM (S/N=6.18) in pH 7 phosphate buffer solution (PBS) at 37 degrees C. The effects of pH, temperature, and enzymatic loading on the sensing characteristics of the UA biosensor were also investigated in this study. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16908130/An_amperometric_uric_acid_biosensor_based_on_modified_Ir_C_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(06)00312-5 DB - PRIME DP - Unbound Medicine ER -