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High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile.
J Exp Bot. 2006; 57(12):3069-78.JE

Abstract

Metabolic manipulation of plants to improve their nutritional quality is an important goal of plant biotechnology. Expression in rice (Oryza sativa L.) of a transgene (OASA1D) encoding a feedback-insensitive alpha subunit of rice anthranilate synthase results in the accumulation of tryptophan (Trp) in calli and leaves. It is shown here that the amount of free Trp in the seeds of such plants is increased by about two orders of magnitude compared with that in the seeds of wild-type plants. The total Trp content in the seeds of the transgenic plants was also increased. Two homozygous lines, HW1 and HW5, of OASA1D transgenic rice were generated for characterization of agronomic traits and aromatic metabolite profiling of seeds. The marked overproduction of Trp was stable in these lines under field conditions, although spikelet fertility and yield, as well as seed germination ability, were reduced compared with the wild type. These differences in agronomic traits were small, however, in HW5. In spite of the high Trp content in the seeds of the HW lines, metabolic profiling revealed no substantial changes in the amounts of other phenolic compounds. The amount of indole acetic acid was increased about 2-fold in the seeds of the transgenic lines. The establishment and characterization of these OASA1D transgenic lines have thus demonstrated the feasibility of increasing the Trp content in the seeds of rice (or of other crops) as a means of improving its nutritional value for human consumption or animal feed.

Authors+Show Affiliations

Department of Rice Breeding, National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan. k3wakasa@nodai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16908506

Citation

Wakasa, Kyo, et al. "High-level Tryptophan Accumulation in Seeds of Transgenic Rice and Its Limited Effects On Agronomic Traits and Seed Metabolite Profile." Journal of Experimental Botany, vol. 57, no. 12, 2006, pp. 3069-78.
Wakasa K, Hasegawa H, Nemoto H, et al. High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. J Exp Bot. 2006;57(12):3069-78.
Wakasa, K., Hasegawa, H., Nemoto, H., Matsuda, F., Miyazawa, H., Tozawa, Y., Morino, K., Komatsu, A., Yamada, T., Terakawa, T., & Miyagawa, H. (2006). High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. Journal of Experimental Botany, 57(12), 3069-78.
Wakasa K, et al. High-level Tryptophan Accumulation in Seeds of Transgenic Rice and Its Limited Effects On Agronomic Traits and Seed Metabolite Profile. J Exp Bot. 2006;57(12):3069-78. PubMed PMID: 16908506.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. AU - Wakasa,Kyo, AU - Hasegawa,Hisakazu, AU - Nemoto,Hiroshi, AU - Matsuda,Fumio, AU - Miyazawa,Haruna, AU - Tozawa,Yuzuru, AU - Morino,Keiko, AU - Komatsu,Akira, AU - Yamada,Tetsuya, AU - Terakawa,Teruhiko, AU - Miyagawa,Hisashi, Y1 - 2006/08/14/ PY - 2006/8/16/pubmed PY - 2006/12/12/medline PY - 2006/8/16/entrez SP - 3069 EP - 78 JF - Journal of experimental botany JO - J Exp Bot VL - 57 IS - 12 N2 - Metabolic manipulation of plants to improve their nutritional quality is an important goal of plant biotechnology. Expression in rice (Oryza sativa L.) of a transgene (OASA1D) encoding a feedback-insensitive alpha subunit of rice anthranilate synthase results in the accumulation of tryptophan (Trp) in calli and leaves. It is shown here that the amount of free Trp in the seeds of such plants is increased by about two orders of magnitude compared with that in the seeds of wild-type plants. The total Trp content in the seeds of the transgenic plants was also increased. Two homozygous lines, HW1 and HW5, of OASA1D transgenic rice were generated for characterization of agronomic traits and aromatic metabolite profiling of seeds. The marked overproduction of Trp was stable in these lines under field conditions, although spikelet fertility and yield, as well as seed germination ability, were reduced compared with the wild type. These differences in agronomic traits were small, however, in HW5. In spite of the high Trp content in the seeds of the HW lines, metabolic profiling revealed no substantial changes in the amounts of other phenolic compounds. The amount of indole acetic acid was increased about 2-fold in the seeds of the transgenic lines. The establishment and characterization of these OASA1D transgenic lines have thus demonstrated the feasibility of increasing the Trp content in the seeds of rice (or of other crops) as a means of improving its nutritional value for human consumption or animal feed. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/16908506/High_level_tryptophan_accumulation_in_seeds_of_transgenic_rice_and_its_limited_effects_on_agronomic_traits_and_seed_metabolite_profile_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erl068 DB - PRIME DP - Unbound Medicine ER -