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Effect of selenium intake and fetal age on mRNA levels of two selenoproteins in porcine fetal and maternal liver.
J Anim Sci 2006; 84(9):2382-90JA

Abstract

The objective of this study was to determine if levels of mRNA encoding cytosolic glutathione peroxidase (cGPx) and thioredoxin reductase (TrxR-1) change during fetal development, and if maternal Se intake during gestation affects the mRNA levels of these proteins. Prepubertal gilts (n = 24) were randomly assigned to either Se-adequate (0.39 ppm of Se; n = 12) or Se-deficient (0.05 ppm of Se; n = 12) diets, 6 wk before breeding. Maternal liver was collected at d 10, 45, 70, and 114 of pregnancy, and fetal liver samples were collected at the same times except d 10. Complementary DNA sequences encoding cGPx and TrxR-1 were cloned and sequenced. Quantitative real-time PCR analysis indicated that levels of mRNA for cGPx in fetal liver decreased more than 3-fold between d 45 and 114 of gestation. Although the gilts were only marginally deficient in Se, and maternal Se intake did not affect cGPx mRNA levels in fetal liver, the low-Se diet tended (P = 0.1) to reduce fetal TrxR-1 mRNA levels. In the liver of the dams, the low Se intake did not affect mRNA levels for either cGPx or TrxR-1. Compared with the liver of the dams, mRNA levels for cGPx were about 3.5 times lower in fetal liver. Results of this study support the hypothesis that neonatal pigs are born with reduced cGPx corresponding to reduced cGPx mRNA levels during late gestation.

Authors+Show Affiliations

School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4660, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

16908641

Citation

Hostetler, C E., et al. "Effect of Selenium Intake and Fetal Age On mRNA Levels of Two Selenoproteins in Porcine Fetal and Maternal Liver." Journal of Animal Science, vol. 84, no. 9, 2006, pp. 2382-90.
Hostetler CE, Michal J, Robison M, et al. Effect of selenium intake and fetal age on mRNA levels of two selenoproteins in porcine fetal and maternal liver. J Anim Sci. 2006;84(9):2382-90.
Hostetler, C. E., Michal, J., Robison, M., Ott, T. L., & Kincaid, R. L. (2006). Effect of selenium intake and fetal age on mRNA levels of two selenoproteins in porcine fetal and maternal liver. Journal of Animal Science, 84(9), pp. 2382-90.
Hostetler CE, et al. Effect of Selenium Intake and Fetal Age On mRNA Levels of Two Selenoproteins in Porcine Fetal and Maternal Liver. J Anim Sci. 2006;84(9):2382-90. PubMed PMID: 16908641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of selenium intake and fetal age on mRNA levels of two selenoproteins in porcine fetal and maternal liver. AU - Hostetler,C E, AU - Michal,J, AU - Robison,M, AU - Ott,T L, AU - Kincaid,R L, PY - 2006/8/16/pubmed PY - 2006/10/26/medline PY - 2006/8/16/entrez SP - 2382 EP - 90 JF - Journal of animal science JO - J. Anim. Sci. VL - 84 IS - 9 N2 - The objective of this study was to determine if levels of mRNA encoding cytosolic glutathione peroxidase (cGPx) and thioredoxin reductase (TrxR-1) change during fetal development, and if maternal Se intake during gestation affects the mRNA levels of these proteins. Prepubertal gilts (n = 24) were randomly assigned to either Se-adequate (0.39 ppm of Se; n = 12) or Se-deficient (0.05 ppm of Se; n = 12) diets, 6 wk before breeding. Maternal liver was collected at d 10, 45, 70, and 114 of pregnancy, and fetal liver samples were collected at the same times except d 10. Complementary DNA sequences encoding cGPx and TrxR-1 were cloned and sequenced. Quantitative real-time PCR analysis indicated that levels of mRNA for cGPx in fetal liver decreased more than 3-fold between d 45 and 114 of gestation. Although the gilts were only marginally deficient in Se, and maternal Se intake did not affect cGPx mRNA levels in fetal liver, the low-Se diet tended (P = 0.1) to reduce fetal TrxR-1 mRNA levels. In the liver of the dams, the low Se intake did not affect mRNA levels for either cGPx or TrxR-1. Compared with the liver of the dams, mRNA levels for cGPx were about 3.5 times lower in fetal liver. Results of this study support the hypothesis that neonatal pigs are born with reduced cGPx corresponding to reduced cGPx mRNA levels during late gestation. SN - 1525-3163 UR - https://www.unboundmedicine.com/medline/citation/16908641/Effect_of_selenium_intake_and_fetal_age_on_mRNA_levels_of_two_selenoproteins_in_porcine_fetal_and_maternal_liver_ L2 - https://academic.oup.com/jas/article-lookup/doi/10.2527/jas.2005-372 DB - PRIME DP - Unbound Medicine ER -