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The non-psychoactive cannabidiol triggers caspase activation and oxidative stress in human glioma cells.
Cell Mol Life Sci. 2006 Sep; 63(17):2057-66.CM

Abstract

Recently, we have shown that the non-psychoactive cannabinoid compound cannabidiol (CBD) induces apoptosis of glioma cells in vitro and tumor regression in vivo. The present study investigated a possible involvement of caspase activation and reactive oxygen species (ROS) induction in the apoptotic effect of CBD. CBD produced a gradual, time-dependent activation of caspase-3, which preceded the appearance of apoptotic death. In addiction, release of cytochrome c and caspase-9 and caspase-8 activation were detected. The exposure to CBD caused in glioma cells an early production of ROS, depletion of intracellular glutathione and increase activity of glutathione reductase and glutathione peroxidase enzymes. Under the same experimental condition, CBD did not impair primary glia. Thus, we found a different sensitivity to the anti-proliferative effect of CBD in human glioma cells and non-transformed cells that appears closely related to a selective ability of CBD in inducing ROS production and caspase activation in tumor cells.

Authors+Show Affiliations

Massi P
Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, via Vanvitelli 32, 20129 Milan, Italy.
Vaccani A
No affiliation info available
Bianchessi S
No affiliation info available
Costa B
No affiliation info available
Macchi P
No affiliation info available
Parolaro D
No affiliation info available

MeSH

CannabidiolCaspasesCell Line, TumorCells, CulturedEnzyme ActivationGliomaGlutathioneHumansOxidative StressReactive Oxygen Species

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16909207

Citation

Massi, P, et al. "The Non-psychoactive Cannabidiol Triggers Caspase Activation and Oxidative Stress in Human Glioma Cells." Cellular and Molecular Life Sciences : CMLS, vol. 63, no. 17, 2006, pp. 2057-66.
Massi P, Vaccani A, Bianchessi S, et al. The non-psychoactive cannabidiol triggers caspase activation and oxidative stress in human glioma cells. Cell Mol Life Sci. 2006;63(17):2057-66.
Massi, P., Vaccani, A., Bianchessi, S., Costa, B., Macchi, P., & Parolaro, D. (2006). The non-psychoactive cannabidiol triggers caspase activation and oxidative stress in human glioma cells. Cellular and Molecular Life Sciences : CMLS, 63(17), 2057-66.
Massi P, et al. The Non-psychoactive Cannabidiol Triggers Caspase Activation and Oxidative Stress in Human Glioma Cells. Cell Mol Life Sci. 2006;63(17):2057-66. PubMed PMID: 16909207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The non-psychoactive cannabidiol triggers caspase activation and oxidative stress in human glioma cells. AU - Massi,P, AU - Vaccani,A, AU - Bianchessi,S, AU - Costa,B, AU - Macchi,P, AU - Parolaro,D, PY - 2006/8/16/pubmed PY - 2006/10/20/medline PY - 2006/8/16/entrez SP - 2057 EP - 66 JF - Cellular and molecular life sciences : CMLS JO - Cell Mol Life Sci VL - 63 IS - 17 N2 - Recently, we have shown that the non-psychoactive cannabinoid compound cannabidiol (CBD) induces apoptosis of glioma cells in vitro and tumor regression in vivo. The present study investigated a possible involvement of caspase activation and reactive oxygen species (ROS) induction in the apoptotic effect of CBD. CBD produced a gradual, time-dependent activation of caspase-3, which preceded the appearance of apoptotic death. In addiction, release of cytochrome c and caspase-9 and caspase-8 activation were detected. The exposure to CBD caused in glioma cells an early production of ROS, depletion of intracellular glutathione and increase activity of glutathione reductase and glutathione peroxidase enzymes. Under the same experimental condition, CBD did not impair primary glia. Thus, we found a different sensitivity to the anti-proliferative effect of CBD in human glioma cells and non-transformed cells that appears closely related to a selective ability of CBD in inducing ROS production and caspase activation in tumor cells. SN - 1420-682X UR - https://www.unboundmedicine.com/medline/citation/16909207/The_non_psychoactive_cannabidiol_triggers_caspase_activation_and_oxidative_stress_in_human_glioma_cells_ L2 - https://doi.org/10.1007/s00018-006-6156-x DB - PRIME DP - Unbound Medicine ER -