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Does intracellular histamine mediate mast cell histamine release?
Biochem Biophys Res Commun. 1990 Mar 16; 167(2):665-72.BB

Abstract

Previously, we demonstrated that through binding a novel intracellular receptor of microM affinity (HIC), histamine mediates, and the HIC antagonist N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine. HCl (DPPE) inhibits, platelet aggregation and serotonin granule secretion; the latter response is dependent upon the same processes that mediate histamine release from mast cell granules. We now show that, as for platelet serotonin release, DPPE blocks concanavalin A-stimulated mast cell histamine release with a potency (IC50 = 30 microM) greater than the H1-antagonist, pyrilamine (IC50 = 150 microM) or the H2-antagonist cimetidine (IC50 = 5 mM), correlating with rank order of potency to inhibit 3H-histamine binding in rat brain membranes and liver microsomes. We postulate that histamine release from mast cells is mediated at HIC by second messenger intracellular histamine. However, unlike platelets, mast cells do not appear to rely on newly synthesized histamine. Rather, as for calcium, histamine may be mobilized from bound stores to mediate histamine secretion.

Authors+Show Affiliations

Brandes LJ
Department of Medicine, University of Manitoba, Winnipeg, Canada.
Sukhu B
No affiliation info available
Bogdanovic RP
No affiliation info available

MeSH

AnimalsCell Membrane PermeabilityCimetidineConcanavalin AHistamineHistamine ReleaseIn Vitro TechniquesMaleMast CellsPhosphatidylserinesPyrilamineRatsRats, Inbred StrainsSaponins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1690990

Citation

Brandes, L J., et al. "Does Intracellular Histamine Mediate Mast Cell Histamine Release?" Biochemical and Biophysical Research Communications, vol. 167, no. 2, 1990, pp. 665-72.
Brandes LJ, Sukhu B, Bogdanovic RP. Does intracellular histamine mediate mast cell histamine release? Biochem Biophys Res Commun. 1990;167(2):665-72.
Brandes, L. J., Sukhu, B., & Bogdanovic, R. P. (1990). Does intracellular histamine mediate mast cell histamine release? Biochemical and Biophysical Research Communications, 167(2), 665-72.
Brandes LJ, Sukhu B, Bogdanovic RP. Does Intracellular Histamine Mediate Mast Cell Histamine Release. Biochem Biophys Res Commun. 1990 Mar 16;167(2):665-72. PubMed PMID: 1690990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Does intracellular histamine mediate mast cell histamine release? AU - Brandes,L J, AU - Sukhu,B, AU - Bogdanovic,R P, PY - 1990/3/16/pubmed PY - 1990/3/16/medline PY - 1990/3/16/entrez SP - 665 EP - 72 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 167 IS - 2 N2 - Previously, we demonstrated that through binding a novel intracellular receptor of microM affinity (HIC), histamine mediates, and the HIC antagonist N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine. HCl (DPPE) inhibits, platelet aggregation and serotonin granule secretion; the latter response is dependent upon the same processes that mediate histamine release from mast cell granules. We now show that, as for platelet serotonin release, DPPE blocks concanavalin A-stimulated mast cell histamine release with a potency (IC50 = 30 microM) greater than the H1-antagonist, pyrilamine (IC50 = 150 microM) or the H2-antagonist cimetidine (IC50 = 5 mM), correlating with rank order of potency to inhibit 3H-histamine binding in rat brain membranes and liver microsomes. We postulate that histamine release from mast cells is mediated at HIC by second messenger intracellular histamine. However, unlike platelets, mast cells do not appear to rely on newly synthesized histamine. Rather, as for calcium, histamine may be mobilized from bound stores to mediate histamine secretion. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/1690990/Does_intracellular_histamine_mediate_mast_cell_histamine_release DB - PRIME DP - Unbound Medicine ER -