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The flagellar-specific transcription factor, sigma28, is the Type III secretion chaperone for the flagellar-specific anti-sigma28 factor FlgM.
Genes Dev. 2006 Aug 15; 20(16):2315-26.GD

Abstract

The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of genes whose products are needed late in assembly until the flagellar basal motor structure, the hook-basal body (HBB), is constructed. A second function for the sigma(28) transcription factor has been discovered: sigma(28) facilitates the secretion of FlgM through the HBB, acting as the FlgM Type III secretion chaperone. Transcription-specific mutants in sigma(28) were isolated that remained competent for FlgM-facilitated secretion separating the transcription and secretion-facilitation activities of sigma (28). Conversely, we also describe the isolation of mutants in sigma(28) that are specific for FlgM-facilitated secretion. The data demonstrate that sigma(28) is the Type III secretion chaperone for its own anti-sigma factor FlgM. Thus, a novel role for a sigma(70)-family transcription factor is described.

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Authors+Show Affiliations

Aldridge PD
Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK. p.d.aldridge@ncl.ac.uk
Karlinsey JE
No affiliation info available
Aldridge C
No affiliation info available
Birchall C
No affiliation info available
Thompson D
No affiliation info available
Yagasaki J
No affiliation info available
Hughes KT
No affiliation info available

MeSH

Bacterial ProteinsDNA-Directed RNA PolymerasesFlagellaMolecular ChaperonesMutagenesisMutationPolymerase Chain ReactionSalmonella typhimuriumSigma Factorbeta-Galactosidase

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16912280

Citation

Aldridge, Phillip D., et al. "The Flagellar-specific Transcription Factor, Sigma28, Is the Type III Secretion Chaperone for the Flagellar-specific Anti-sigma28 Factor FlgM." Genes & Development, vol. 20, no. 16, 2006, pp. 2315-26.
Aldridge PD, Karlinsey JE, Aldridge C, et al. The flagellar-specific transcription factor, sigma28, is the Type III secretion chaperone for the flagellar-specific anti-sigma28 factor FlgM. Genes Dev. 2006;20(16):2315-26.
Aldridge, P. D., Karlinsey, J. E., Aldridge, C., Birchall, C., Thompson, D., Yagasaki, J., & Hughes, K. T. (2006). The flagellar-specific transcription factor, sigma28, is the Type III secretion chaperone for the flagellar-specific anti-sigma28 factor FlgM. Genes & Development, 20(16), 2315-26.
Aldridge PD, et al. The Flagellar-specific Transcription Factor, Sigma28, Is the Type III Secretion Chaperone for the Flagellar-specific Anti-sigma28 Factor FlgM. Genes Dev. 2006 Aug 15;20(16):2315-26. PubMed PMID: 16912280.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The flagellar-specific transcription factor, sigma28, is the Type III secretion chaperone for the flagellar-specific anti-sigma28 factor FlgM. AU - Aldridge,Phillip D, AU - Karlinsey,Joyce E, AU - Aldridge,Christine, AU - Birchall,Christopher, AU - Thompson,Danielle, AU - Yagasaki,Jin, AU - Hughes,Kelly T, PY - 2006/8/17/pubmed PY - 2006/9/20/medline PY - 2006/8/17/entrez SP - 2315 EP - 26 JF - Genes & development JO - Genes Dev VL - 20 IS - 16 N2 - The sigma(28) protein is a member of the bacterial sigma(70)-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The sigma(28) protein is regulated in response to flagellar assembly by the anti-sigma(28) factor FlgM. FlgM inhibits sigma(28)-dependent transcription of genes whose products are needed late in assembly until the flagellar basal motor structure, the hook-basal body (HBB), is constructed. A second function for the sigma(28) transcription factor has been discovered: sigma(28) facilitates the secretion of FlgM through the HBB, acting as the FlgM Type III secretion chaperone. Transcription-specific mutants in sigma(28) were isolated that remained competent for FlgM-facilitated secretion separating the transcription and secretion-facilitation activities of sigma (28). Conversely, we also describe the isolation of mutants in sigma(28) that are specific for FlgM-facilitated secretion. The data demonstrate that sigma(28) is the Type III secretion chaperone for its own anti-sigma factor FlgM. Thus, a novel role for a sigma(70)-family transcription factor is described. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/16912280/The_flagellar_specific_transcription_factor_sigma28_is_the_Type_III_secretion_chaperone_for_the_flagellar_specific_anti_sigma28_factor_FlgM_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=16912280 DB - PRIME DP - Unbound Medicine ER -