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In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus.
Arch Oral Biol 2006; 51(12):1131-40AO

Abstract

OBJECTIVES

Polygonum cuspidatum has been used in Korean folk medicine to improve oral hygiene. This study was performed to evaluate the effects of methanol extract from root of P. cuspidatum (MEP) on bacterial viability and the virulence factors of Streptococcus mutans and Streptococcus sobrinus.

METHODS

To test the effects of MEP on bacterial viability, we determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against 20 bacterial strains, including S. mutans and S. sobrinus, using a micro-dilution assay. In case of S. mutans and S. sobrinus, the assays for time-kill and bacterial growth rate at sub-MIC concentrations were also performed. To determine effects of the extract on the virulence factors of S. mutans and S. sobrinus, the assays for sucrose-dependent adherence, water-insoluble glucan formation, glycolytic acid production, and acid tolerance were performed at sub-MIC levels. Phytochemical analysis for constituents of MEP was carried out.

RESULTS

MEP showed a broad antibacterial range (MIC 0.5-4 mg/ml). The MBC was two to four times higher than the MIC. The time-kill curves showed S. mutans and S. sobrinus were significantly killed after 1h of incubation. At sub-MIC levels, doubling times of S. mutans and S. sobrinus dose-dependently increased up to 211% and 123%, respectively. At sub-MIC levels, MEP also showed inhibitory effects on the virulence factors of S. mutans and S. sobrinus in a dose-dependent fashion. Phytochemical analysis revealed the presence of alkaloids, sterol/terpenes, tannins, flavonoids, and carbohydrates.

CONCLUSION

These data indicate that MEP has inhibitory effects on bacterial viability at higher concentrations (> or =MIC) and the virulence factors of S. mutans and S. sobrinus at sub-MIC concentrations, suggesting that it might be useful for the control of dental plaque formation and subsequent dental caries formation.

Authors+Show Affiliations

Department of Preventive Dentistry, School of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju 561-756, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16914113

Citation

Song, Ju-Hee, et al. "In Vitro Inhibitory Effects of Polygonum Cuspidatum On Bacterial Viability and Virulence Factors of Streptococcus Mutans and Streptococcus Sobrinus." Archives of Oral Biology, vol. 51, no. 12, 2006, pp. 1131-40.
Song JH, Kim SK, Chang KW, et al. In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus. Arch Oral Biol. 2006;51(12):1131-40.
Song, J. H., Kim, S. K., Chang, K. W., Han, S. K., Yi, H. K., & Jeon, J. G. (2006). In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus. Archives of Oral Biology, 51(12), pp. 1131-40.
Song JH, et al. In Vitro Inhibitory Effects of Polygonum Cuspidatum On Bacterial Viability and Virulence Factors of Streptococcus Mutans and Streptococcus Sobrinus. Arch Oral Biol. 2006;51(12):1131-40. PubMed PMID: 16914113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus. AU - Song,Ju-Hee, AU - Kim,Shin-Kyu, AU - Chang,Kee-Wan, AU - Han,Seong-Kyu, AU - Yi,Ho-Keun, AU - Jeon,Jae-Gyu, Y1 - 2006/08/17/ PY - 2006/04/18/received PY - 2006/06/26/revised PY - 2006/06/27/accepted PY - 2006/8/18/pubmed PY - 2007/10/13/medline PY - 2006/8/18/entrez SP - 1131 EP - 40 JF - Archives of oral biology JO - Arch. Oral Biol. VL - 51 IS - 12 N2 - OBJECTIVES: Polygonum cuspidatum has been used in Korean folk medicine to improve oral hygiene. This study was performed to evaluate the effects of methanol extract from root of P. cuspidatum (MEP) on bacterial viability and the virulence factors of Streptococcus mutans and Streptococcus sobrinus. METHODS: To test the effects of MEP on bacterial viability, we determined the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against 20 bacterial strains, including S. mutans and S. sobrinus, using a micro-dilution assay. In case of S. mutans and S. sobrinus, the assays for time-kill and bacterial growth rate at sub-MIC concentrations were also performed. To determine effects of the extract on the virulence factors of S. mutans and S. sobrinus, the assays for sucrose-dependent adherence, water-insoluble glucan formation, glycolytic acid production, and acid tolerance were performed at sub-MIC levels. Phytochemical analysis for constituents of MEP was carried out. RESULTS: MEP showed a broad antibacterial range (MIC 0.5-4 mg/ml). The MBC was two to four times higher than the MIC. The time-kill curves showed S. mutans and S. sobrinus were significantly killed after 1h of incubation. At sub-MIC levels, doubling times of S. mutans and S. sobrinus dose-dependently increased up to 211% and 123%, respectively. At sub-MIC levels, MEP also showed inhibitory effects on the virulence factors of S. mutans and S. sobrinus in a dose-dependent fashion. Phytochemical analysis revealed the presence of alkaloids, sterol/terpenes, tannins, flavonoids, and carbohydrates. CONCLUSION: These data indicate that MEP has inhibitory effects on bacterial viability at higher concentrations (> or =MIC) and the virulence factors of S. mutans and S. sobrinus at sub-MIC concentrations, suggesting that it might be useful for the control of dental plaque formation and subsequent dental caries formation. SN - 0003-9969 UR - https://www.unboundmedicine.com/medline/citation/16914113/In_vitro_inhibitory_effects_of_Polygonum_cuspidatum_on_bacterial_viability_and_virulence_factors_of_Streptococcus_mutans_and_Streptococcus_sobrinus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(06)00160-9 DB - PRIME DP - Unbound Medicine ER -