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Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections.
Int J Infect Dis. 2007 May; 11(3):256-62.IJ

Abstract

OBJECTIVES

The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue infection were studied.

DESIGN

Serum, saliva, and urine samples were collected from 22 patients with clinical and confirmed dengue 3 virus infection during the outbreak in Havana City in 2001. They were tested by capture IgM (MAC-ELISA), IgA (AAC-ELISA), and IgE (EAC-ELISA) and IgG ELISA inhibition method (EIM) to detect specific dengue antibodies.

RESULTS

Similar kinetics were observed in IgM, IgA, and IgG antibodies in saliva and IgA and IgG in urine samples from secondary cases compared with kinetics in serum samples, although the values were lower. No IgG antibody was detected in saliva and urine samples in primary cases and IgM antibody was not detected in urine samples from either primary or secondary infection. All secondary cases were positive for IgG in saliva and urine samples at day 7. The kinetics of specific IgE antibodies in primary and secondary cases were different.

CONCLUSIONS

The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infection were studied for the first time, showing its behavior and usefulness in dengue virus diagnosis. The specific IgE could play a role as a serological marker in secondary infections.

Authors+Show Affiliations

"Pedro Kourí" Tropical Medicine Institute, PAHO/WHO Collaborating Center for Dengue and its Vectors, Autopista Novia del Mediodía, Km 6 1/2, La Lisa, Havana City, Cuba. svazquez@ipk.sld.cuNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16914345

Citation

Vázquez, S, et al. "Kinetics of Antibodies in Sera, Saliva, and Urine Samples From Adult Patients With Primary or Secondary Dengue 3 Virus Infections." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 11, no. 3, 2007, pp. 256-62.
Vázquez S, Cabezas S, Pérez AB, et al. Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. Int J Infect Dis. 2007;11(3):256-62.
Vázquez, S., Cabezas, S., Pérez, A. B., Pupo, M., Ruiz, D., Calzada, N., Bernardo, L., Castro, O., González, D., Serrano, T., Sanchez, A., & Guzmán, M. G. (2007). Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 11(3), 256-62.
Vázquez S, et al. Kinetics of Antibodies in Sera, Saliva, and Urine Samples From Adult Patients With Primary or Secondary Dengue 3 Virus Infections. Int J Infect Dis. 2007;11(3):256-62. PubMed PMID: 16914345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections. AU - Vázquez,S, AU - Cabezas,S, AU - Pérez,A B, AU - Pupo,M, AU - Ruiz,D, AU - Calzada,N, AU - Bernardo,L, AU - Castro,O, AU - González,D, AU - Serrano,T, AU - Sanchez,A, AU - Guzmán,M G, Y1 - 2006/08/17/ PY - 2006/01/13/received PY - 2006/04/22/revised PY - 2006/05/02/accepted PY - 2006/8/18/pubmed PY - 2007/7/10/medline PY - 2006/8/18/entrez SP - 256 EP - 62 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 11 IS - 3 N2 - OBJECTIVES: The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue infection were studied. DESIGN: Serum, saliva, and urine samples were collected from 22 patients with clinical and confirmed dengue 3 virus infection during the outbreak in Havana City in 2001. They were tested by capture IgM (MAC-ELISA), IgA (AAC-ELISA), and IgE (EAC-ELISA) and IgG ELISA inhibition method (EIM) to detect specific dengue antibodies. RESULTS: Similar kinetics were observed in IgM, IgA, and IgG antibodies in saliva and IgA and IgG in urine samples from secondary cases compared with kinetics in serum samples, although the values were lower. No IgG antibody was detected in saliva and urine samples in primary cases and IgM antibody was not detected in urine samples from either primary or secondary infection. All secondary cases were positive for IgG in saliva and urine samples at day 7. The kinetics of specific IgE antibodies in primary and secondary cases were different. CONCLUSIONS: The kinetics of three serological markers (IgM, IgA, and IgG) in serum, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infection were studied for the first time, showing its behavior and usefulness in dengue virus diagnosis. The specific IgE could play a role as a serological marker in secondary infections. SN - 1201-9712 UR - https://www.unboundmedicine.com/medline/citation/16914345/Kinetics_of_antibodies_in_sera_saliva_and_urine_samples_from_adult_patients_with_primary_or_secondary_dengue_3_virus_infections_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(06)00120-2 DB - PRIME DP - Unbound Medicine ER -