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Up-regulation of acid-sensing ion channel 3 in dorsal root ganglion neurons following application of nucleus pulposus on nerve root in rats.
Spine (Phila Pa 1976). 2006 Aug 15; 31(18):2048-52.S

Abstract

STUDY DESIGN

Immunocytochemistry for acid-sensing ion channel 3 (ASIC3) in neurons of rat dorsal root ganglions (DRGs) from animals exposed to a model of lumbar disc herniation.

OBJECTIVE

To examine expression of ASIC3 in DRGs and the effect of a sodium channel blocker applied to the nerve root in a rat model of lumbar disc herniation.

SUMMARY OF BACKGROUND DATA

Radicular pain is a common symptom of lumbar disc herniation in human beings. A depolarizing sodium channel gated by protons during tissue acidosis, ASIC3, is specifically expressed in sensory neurons. It has been associated with cardiac ischemic and inflammatory pain. We often perform spinal nerve root block for radicular pain using a sodium channel blocker, such as lidocaine; however, it has been unclear whether the effective period of this treatment is usually longer than the expected duration of efficacy.

METHODS

For the lumbar disc herniation model, nucleus pulposus was harvested from the tail and applied to the L5 nerve root, and the nerve roots were pinched. We evaluated mechanical allodynia in sham-operated animals and a disc herniation model. Immunohistochemistry was used to examine ASIC3 expression in L5 DRGs. Finally, the effect of lidocaine on pain and ASIC3 expression in the disc herniation model was examined.

RESULTS

Animals exposed to the lumbar disc herniation model showed allodynia for 8 days, and ASIC3 immunoreactivity was up-regulated in DRG neurons. After administration of lidocaine to spinal nerve roots affected by disc herniation, ASIC3 immunoreactivity was down-regulated in DRG neurons, and the level of mechanical allodynia was significantly decreased for 8 days.

CONCLUSIONS

Our results suggest that ASIC3 in DRG neurons may play an important role in nerve root pain caused by lumbar disc herniation. Lidocaine decreased ASIC3 expression in DRG neurons and pain associated with the disc herniation model.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan. sohtori@faculty.chiba-u.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16915087

Citation

Ohtori, Seiji, et al. "Up-regulation of Acid-sensing Ion Channel 3 in Dorsal Root Ganglion Neurons Following Application of Nucleus Pulposus On Nerve Root in Rats." Spine, vol. 31, no. 18, 2006, pp. 2048-52.
Ohtori S, Inoue G, Koshi T, et al. Up-regulation of acid-sensing ion channel 3 in dorsal root ganglion neurons following application of nucleus pulposus on nerve root in rats. Spine (Phila Pa 1976). 2006;31(18):2048-52.
Ohtori, S., Inoue, G., Koshi, T., Ito, T., Doya, H., Saito, T., Moriya, H., & Takahashi, K. (2006). Up-regulation of acid-sensing ion channel 3 in dorsal root ganglion neurons following application of nucleus pulposus on nerve root in rats. Spine, 31(18), 2048-52.
Ohtori S, et al. Up-regulation of Acid-sensing Ion Channel 3 in Dorsal Root Ganglion Neurons Following Application of Nucleus Pulposus On Nerve Root in Rats. Spine (Phila Pa 1976). 2006 Aug 15;31(18):2048-52. PubMed PMID: 16915087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Up-regulation of acid-sensing ion channel 3 in dorsal root ganglion neurons following application of nucleus pulposus on nerve root in rats. AU - Ohtori,Seiji, AU - Inoue,Gen, AU - Koshi,Takana, AU - Ito,Toshinori, AU - Doya,Hideo, AU - Saito,Tomoko, AU - Moriya,Hideshige, AU - Takahashi,Kazuhisa, PY - 2006/8/18/pubmed PY - 2006/9/1/medline PY - 2006/8/18/entrez SP - 2048 EP - 52 JF - Spine JO - Spine (Phila Pa 1976) VL - 31 IS - 18 N2 - STUDY DESIGN: Immunocytochemistry for acid-sensing ion channel 3 (ASIC3) in neurons of rat dorsal root ganglions (DRGs) from animals exposed to a model of lumbar disc herniation. OBJECTIVE: To examine expression of ASIC3 in DRGs and the effect of a sodium channel blocker applied to the nerve root in a rat model of lumbar disc herniation. SUMMARY OF BACKGROUND DATA: Radicular pain is a common symptom of lumbar disc herniation in human beings. A depolarizing sodium channel gated by protons during tissue acidosis, ASIC3, is specifically expressed in sensory neurons. It has been associated with cardiac ischemic and inflammatory pain. We often perform spinal nerve root block for radicular pain using a sodium channel blocker, such as lidocaine; however, it has been unclear whether the effective period of this treatment is usually longer than the expected duration of efficacy. METHODS: For the lumbar disc herniation model, nucleus pulposus was harvested from the tail and applied to the L5 nerve root, and the nerve roots were pinched. We evaluated mechanical allodynia in sham-operated animals and a disc herniation model. Immunohistochemistry was used to examine ASIC3 expression in L5 DRGs. Finally, the effect of lidocaine on pain and ASIC3 expression in the disc herniation model was examined. RESULTS: Animals exposed to the lumbar disc herniation model showed allodynia for 8 days, and ASIC3 immunoreactivity was up-regulated in DRG neurons. After administration of lidocaine to spinal nerve roots affected by disc herniation, ASIC3 immunoreactivity was down-regulated in DRG neurons, and the level of mechanical allodynia was significantly decreased for 8 days. CONCLUSIONS: Our results suggest that ASIC3 in DRG neurons may play an important role in nerve root pain caused by lumbar disc herniation. Lidocaine decreased ASIC3 expression in DRG neurons and pain associated with the disc herniation model. SN - 1528-1159 UR - https://www.unboundmedicine.com/medline/citation/16915087/Up_regulation_of_acid_sensing_ion_channel_3_in_dorsal_root_ganglion_neurons_following_application_of_nucleus_pulposus_on_nerve_root_in_rats_ L2 - https://doi.org/10.1097/01.brs.0000231756.56230.13 DB - PRIME DP - Unbound Medicine ER -